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159,258 results

Showing: 8761 - 8800 of 159258 results

  1. pSAB35

    Plasmid
    #175787
    Purpose
    Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag
    Depositor
    John Rubinstein
    Article
    Abbas et al Science. 2020 Mar 13;367(6483):1240-1246
    Insert
    SidK (1-278)
    Use
    Tags
    3xFLAG and 6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    175787-plasmid-map-sequence-id-348166

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  3. Tol2-CAG::Mitbow

    Plasmid
    #158994
    Purpose
    Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Mitbow
    Use
    Tags
    The default expressed EBFP2 is fused to H2B; oth…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158994-plasmid-map-sequence-id-311800

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  8. pLVTHM

    Plasmid
    #12247
    Purpose
    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid
    Depositor
    Didier Trono
    Article
    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12247-plasmid-map-sequence-id-352425

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  11. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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  13. pDB5321

    Plasmid
    #204831
    Purpose
    An integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.
    Depositor
    Li-Lin Du
    Article
    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404
    Inserts
    tetR
    mECitrine
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    CMV promoter and enotetSW3 promoter
    Available since
    Aug. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    204831-plasmid-map-sequence-id-405723

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  16. pLKO-Tet-ON-shCtrl (neo)

    Plasmid
    #192076
    Purpose
    Lentivirus for Dox-inducible expression of a non-targeting control shRNA
    Depositor
    Roland Friedel
    Insert
    non-targeting randomized sequence
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    H1 / Tet-responsive element
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192076-plasmid-map-sequence-id-379241

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  21. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177941-plasmid-map-sequence-id-348207

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  23. EK0007-PB-4XnrUAS-H2B-BFP

    Plasmid
    #236121
    Purpose
    Fuorescent reporter encoding H2B-tagBFP fusion under control of E1b minimal promoter with four Gal4-binding sites upstream
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with four Gal4-binding sites…
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236121-plasmid-map-sequence-id-468461

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  27. pQCXIP-mCherry-CDMPR

    Plasmid
    #182643
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    CDMPR
    Use
    Retroviral
    Tags
    N-terminal ER targeting sequence of hemagglutinin…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182643-plasmid-map-sequence-id-358629

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  29. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

    Plasmid
    #125256
    Purpose
    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection
    Depositor
    Roger D. Kamm
    Article
    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi
    Insert
    hChR2(H134R)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-forward
    Available since
    May 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    125256-plasmid-map-sequence-id-245091

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  30. ePPEplus

    Plasmid
    #205241
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205241-plasmid-map-sequence-id-408831

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  31. pG108 - K

    Plasmid
    #178039
    Purpose
    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers
    Depositor
    Janina Lewis
    Article
    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1
    Insert
    Kanamycin resistance
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ErmAM has been replaced with Kanamycin for select…
    Promoter
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    178039-plasmid-map-sequence-id-378887

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  37. pET28-MKH8SUMO

    Plasmid
    #79526
    Purpose
    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    8xHis-Thrombin-SUMO-TEV and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197026-plasmid-map-sequence-id-394112

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Showing: 8761 - 8800 of 159258 results