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We narrowed to 13,583 results for: TIM

Showing: 11651 - 11700 of 13583 results

  3. AMA1-bio

    Plasmid
    #47741
    Purpose
    Expresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised AMA1
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47741-plasmid-map-sequence-id-68749

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  7. pTE2690

    Plasmid
    #137717
    Purpose
    Expresses dimpLbCas12a-SunTag in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576
    Insert
    dimpLbCas12a
    Use
    CRISPR
    Tags
    NLSx3 SV40 and SunTagx10
    Expression
    Mammalian
    Mutation
    D156R, G532R, K538V, Y542R, K595R, D832A,
    Promoter
    CMV
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137717-plasmid-map-sequence-id-274029

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  13. MSP10-bio

    Plasmid
    #47719
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP10
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47719-plasmid-map-sequence-id-68458

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  14. pcDNA3-SARS-1-RBD-8h

    Plasmid
    #161821
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Use
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T320-D518)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161821-plasmid-map-sequence-id-316749

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  16. pMP926

    Plasmid
    #135001
    Purpose
    m6A Reader-Writer. Propagates Gm6ATC along GATC arrays as well as across cell generations. pUBC-DpnI(aa146-254)-3xFLAG-Dam(R95A) pGK-HygroR
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pUBC-DpnI(aa146-254)-3XFLAG-Dam(R95A) pGK-HygroR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG, Dam(R95A), and DpnI(aa146-254)
    Expression
    Mammalian
    Mutation
    DpnI domain truncated to aa146-254. Dam protein m…
    Promoter
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    135001-plasmid-map-sequence-id-267929

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  18. pcDNA3-LYRa11-RBD-8h

    Plasmid
    #161822
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Use
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T324-D522)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161822-plasmid-map-sequence-id-316758

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  19. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

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  23. pBJG1 T7-8xHis-OGT D554N

    Plasmid
    #154279
    Purpose
    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Aspartate 554 changed to Asparagine- bacterial co…
    Promoter
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154279-plasmid-map-sequence-id-304834

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  24. NSP14-Flag

    Plasmid
    #210346
    Purpose
    Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells
    Depositor
    Michele Pagano
    Article
    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1
    Insert
    SARS-CoV-2 NSP14 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    210346-plasmid-map-sequence-id-418213

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  25. pcDNA3-RsSHC014-RBD-8h

    Plasmid
    #161826
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Use
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T321-D519)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161826-plasmid-map-sequence-id-316777

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  30. pcDNA3-HCMVgO-sfGFP (Merlin)

    Plasmid
    #136452
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Use
    Tags
    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136452-plasmid-map-sequence-id-269876

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  32. p-mCherry-C1-iCoChR++

    Plasmid
    #98173
    Purpose
    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iCoChR++ gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T24…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98173-plasmid-map-sequence-id-182757

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  34. MTRAP-bio

    Plasmid
    #47746
    Purpose
    Expresses enzymatically monobiotinylated full-length MTRAP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MTRAP
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47746-plasmid-map-sequence-id-68760

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  39. pHS1801

    Plasmid
    #205966
    Purpose
    JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    Cas8-HNH proteins and CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac (proteins), pJ23119 (crRNA)
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205966-plasmid-map-sequence-id-416251

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  40. pHS1232

    Plasmid
    #205969
    Purpose
    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    Cas5-HNH proteins and CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac (proteins), pJ23119 (crRNA)
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205969-plasmid-map-sequence-id-416250

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  42. pcDNA3-Rs7327-RBD-8h

    Plasmid
    #161823
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Use
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T321-D519)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161823-plasmid-map-sequence-id-316772

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  46. EBA140-bio

    Plasmid
    #47742
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA140
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47742-plasmid-map-sequence-id-68750

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  49. MERS-coV-NSP3-c001

    Plasmid
    #173085
    Purpose
    Expression of recombinant fusion protein in E. coli
    Depositor
    Lari Lehtiö
    Article
    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012
    Insert
    rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
    Use
    Tags
    mCerulean
    Expression
    Bacterial
    Mutation
    D1109-D1275, codon optimized
    Promoter
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    173085-plasmid-map-sequence-id-349919

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Showing: 11651 - 11700 of 13583 results