177,138 results
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Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only
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pSEVA331Bb
Plasmid#78269PurposepSEVA331 with Biobrick multiple cloning siteDepositorTypeEmpty backboneAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET_sfCherry(1-10)_32aalinker_sfCherry(11)
Plasmid#83030PurposeBacterial expression vector for split sfCherry screeningDepositorInsertsfCherry(1-10)_32aalinker_sfCherry(11)
ExpressionBacterialPromoterT7 promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAS9-mCherry empty
Plasmid#80975PurposeCas9, mCherry, and sgRNA expressionDepositorTypeEmpty backboneExpressionMammalianAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUB_smFLAG_KDM5B_MS2
Plasmid#81084PurposeTranslation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_KDM5B_24xMS2
ExpressionMammalianAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDF-His-mTET1CD
Plasmid#81053Purposebacterial expression of the catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
Tags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057PromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
WN10151
Plasmid#80441PurposeExpresses human codon-optimized dead (832A) LbCpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdead (D832A) LbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832AAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.cytGAP
Plasmid#78734PurposeExpression of Ca2+ sensor GAP in the cytosolDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcytGAP
TagsluciferaseExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMAL-p2X
Plasmid#75287PurposeExpresses proteins in the periplasm as fusions to maltose-binding proteinDepositorTypeEmpty backboneTagsmaltose-binding proteinExpressionBacterialPromotertacAvailable sinceJuly 18, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pTiGc
Plasmid#78314PurposeMycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).DepositorInsertsTurboFP635
GFP
UseSynthetic Biology; Mycobacterial replicating vect…ExpressionBacterialPromoterhsp60 and hsp60(ribo)Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
psiCHECK2-let-7 WT
Plasmid#78260PurposepsiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfully complimentary let-7 target site
UseLuciferase; Microrna activityExpressionMammalianAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG-FADD
Plasmid#78802PurposeTo overexpress FADD in Mammalian CellsDepositorAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEMS2115
Plasmid#49140PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-emGFP-WPRE
UseAAVExpressionMammalianPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pAC150-PBLHL-4xHS-EF1a-DEST
Plasmid#48234PurposePiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection markerDepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromoterEF1aAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti.CMV/TO_PRKAA1
Plasmid#74446PurposeLentiviral expression vector encoding untagged WT human AMPK alpha 1DepositorAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Myc-AIM2
Plasmid#73958PurposeExpress c-Myc-tagged human AIM2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertabsent in melanoma 2 (AIM2) (AIM2 Human)
Tagsc-mycExpressionMammalianPromoterCMV iEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-Peredox
Plasmid#73807PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)DepositorInsertPeredox
UseAAVExpressionMammalianAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMpGE_En03
Plasmid#71535PurposeUsed to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-KozATG-dL5-2XG4S-mCer3
Plasmid#73207PurposeExpresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKozATG-dL5-2XG4S-mCer3
TagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIP-F-luc
Plasmid#73058PurposeRetroviral expression of firefly luciferase for in vivo imagingDepositorInsertFirefly luciferase
UseRetroviralExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-Y701F-STAT1
Plasmid#71453PurposeLentiviral expression of Y701F-STAT1DepositorAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA_Lifeact-GFP_NLS-mCherry
Plasmid#69058PurposeDual Fluorescent Reporter for F-actin and NucleiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeAct-GFP_IRES_NLS-mCherry
TagsLifeact-GFP, NLS-mCherryExpressionMammalianPromoterCMVAvailable sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHBV-X/EnhI-Luc
Plasmid#71418PurposeTo see HBV X promoter(overlaped EnhanceI) activity for luciferase assayDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHBV X promoter and enhancer I driving Luciferase expression
UseLuciferaseExpressionMammalianPromoterHBV XAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-DDdCas9VP192-T2A-EGFP-ires-puro
Plasmid#69534PurposeDHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDD-dCas9VP192-T2A-EGFP
TagsC-term T2A-EGFP and DHFR Destabilised DomainExpressionMammalianPromoterCAGAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SOX2se-CPG-TV
Plasmid#70155Purposea targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyADepositorInsert5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
UseMouse Targeting and Synthetic BiologyAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pST39
Plasmid#64009Purposeoriginal polycistronic cloning vector for plasmid suite (generation I)DepositorTypeEmpty backboneExpressionBacterialPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO_FOXA1_#2
Plasmid#70096PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMT-Bip-GFP:V5:KDEL
Plasmid#69917PurposeAn endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.DepositorInsertGFP
TagsDrosophila BiP signal sequence, KDEL ER targeting…ExpressionInsectPromoterpMTAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HTR1A-Tango
Plasmid#66404PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTR1A (HTR1A Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
FFA1-Tango
Plasmid#66280PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFFA1 (FFAR1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CCR2-Tango
Plasmid#66239PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCCR2 (CCR2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CHRM1-Tango
Plasmid#66248PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCHRM1 (CHRM1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OCT4-2A-mOrange
Plasmid#66986PurposeGenerate OCT4 mOrange reporter line without selection cassetteDepositorInsert2A-mOrange
Available sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BLBP-GFP
Plasmid#63174PurposeExpressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBLBP (Fabp7 Mouse)
ExpressionMammalianPromoter1700bp upstream of BLBP geneAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFRT-TODestRFP_RNAseH1
Plasmid#65785Purposeexpresses RFP-tagged RNAseH1 in mammalian cellsDepositorAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGH00.0126
Plasmid#64257PurposeBinary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformationDepositorInsertsEGFP
neomycin phosphotransferase II
UseBinary agrobacterium vector for plant transformat…ExpressionPlantPromoterE12-omegaAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABEPuro-HA-GLI1
Plasmid#62967PurposeExpresses HA-tagged Gli1DepositorAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLncEXP
Plasmid#64865PurposeEmpty vector for lncRNA expressionDepositorTypeEmpty backboneUseRetroviralPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-TRIM24
Plasmid#65396PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTRIM24 (TRIM24 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-AID
Plasmid#61926PurposeExpression of human AID tagged with EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAICDA (AICDA Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
WNV VLPs pVRC8400
Plasmid#63160PurposeWest Nile Virus prM-E structural cassette (NY99 sequence) in pVRC8400DepositorInsertWNV prM-E
ExpressionMammalianAvailable sinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BLBP-mCherry
Plasmid#63721PurposeExpressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBLBP (Fabp7 Mouse)
ExpressionMammalianMutation1700 bp upstream of BLBP gene cloned in CAG-mCher…Promoter1700bp upstream of BLBP geneAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (full-length, aa 1-481)
Plasmid#61405PurposeExpresses human OTUD1 (full-length) in E. coli.DepositorAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINK-OTUD2 (full-length, aa 1-348)
Plasmid#61409PurposeExpresses human OTUD2 (full-length) in E. coli.DepositorAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
LRP8-bio-His
Plasmid#51879PurposeExpresses full-length Low-density lipoprotein receptor-related protein 8 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertLRP8 (LRP8 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-BB
Plasmid#60521PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBtet-BB
Plasmid#60505PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEX-PK-hLC3∆G
Plasmid#61459PurposeExpress pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagyDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPK-hLC3∆G (MAP1LC3B Human)
ExpressionMammalianPromoterCAGAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEos2-Mito-7
Plasmid#57401PurposeLocalization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMito (COX8A Human)
TagsmEos2ExpressionMammalianPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Human NANOS3-P2A-mCherry knock-in targeting construct
Plasmid#59721PurposeGene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.DepositorInsertP2A mCherry
ExpressionMammalianAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by