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20,202 results

Showing: 9881 - 9920 of 20202 results

  7. PB-TAG-ERN

    Plasmid
    #80476
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80476-plasmid-map-sequence-id-153664

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  21. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Article
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available Since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  29. p406GAL1

    Plasmid
    #17437
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17437-plasmid-map-sequence-id-8611

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  31. pSC101_TIMER

    Plasmid
    #103057
    Purpose
    low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)
    Depositor
    Dirk Bumann
    Article
    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1
    Insert
    TIMER-bac
    Expression
    Bacterial
    Mutation
    mutations: S4T and S179T
    Promoter
    constitutive ybaJp promoter
    Available Since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103057-plasmid-map-sequence-id-199056

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  35. SpyLigase

    Plasmid
    #51722
    Purpose
    Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SpyLigase
    Tags
    His6 Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51722-plasmid-map-sequence-id-251770

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  40. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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Showing: 9881 - 9920 of 20202 results