164,223 results

1. pUC18R6K-mini-Tn7T-Gm

   Plasmid

   #65022

   Purpose

   GmR mini-Tn7 vector, R6K replicon

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 17, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. eTC GFP beta-actin full length

   Plasmid

   #27123

   Depositor

   Robert Singer

   Article

   Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

   Insert

   beta-actin (ACTB Human)
   

   Use

   Tags

   EGFP and TC tag

   Expression

   Mammalian

   Mutation

   E237A

   Promoter

   Available Since

   Jan. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBacDual with PA+Histag

   Plasmid

   #84870

   Purpose

   Baculovirus Expression of Bacillus anthracis protective antigen

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Insert

   PA
   

   Use

   Baculovirus

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. mVenus-IQ6 WT

   Plasmid

   #198201

   Purpose

   FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   MYO5A IQ domain (Myo5a Mouse)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-TLR6-YFP

   Plasmid

   #13020

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 6 (TLR6 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166015

   Purpose

   mammalian expression of HCoV-OC43 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   HCoV-OC43 S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. ADORA2B-DuET

   Plasmid

   #213175

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADORA2B (ADORA2B Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-ERBB4-V5/HIS

   Plasmid

   #201105

   Purpose

   expression of human ERBB4 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human ERBB4 receptor tyrosine kinase, full length, wildtype (ERBB4 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-NES-KIRIN1

   Plasmid

   #107416

   Purpose

   Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid

    #74707

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

    Plasmid

    #185447

    Purpose

    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Inserts

    TRF1 (TERF1 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    

    Use

    Tags

    MBP, YBBR, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBSV2_P0026-mCherryBb

    Plasmid

    #118239

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    bb0026 promoter driven mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-PGK- dCas9-SunTag-P2A-HygR

    Plasmid

    #193137

    Purpose

    Expresses dCas9 fused to the SunTag scaffold

    Depositor

    Brad Rosenberg

    Article

    Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

    Insert

    dCas9-SunTag-T2A-HygR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-Mito4x-jRCaMP1b

    Plasmid

    #127873

    Purpose

    Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Red-shifted mitochondrial matrix Ca2+ indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLifeAct_mScarlet_N1

    Plasmid

    #85054

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV/SB11

    Plasmid

    #26552

    Depositor

    Perry Hackett

    Article

    Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

    Insert

    Sleeping Beauty
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Methionine 243 to Glutamine Threonine 136 to Arg…

    Promoter

    Available Since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/V5-His Snk/Plk2

    Plasmid

    #16015

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Plasmid

    #180475

    Purpose

    Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    Zebrafish ubb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PDE9A_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176945

    Purpose

    Mammalian expression vector encoding PDE9A and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    PDE9A (PDE9A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCACTUS

    Plasmid

    #167559

    Purpose

    Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.

    Depositor

    Renato Morona

    Article

    Morona et al J Bacteriol. 1995 Feb;177(4):1059-68. do

    Type

    Empty backbone

    Use

    Suicide cloning vector, temperature sensitive.

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDB1 (CK2alpha)

    Plasmid

    #27083

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha (CSNK2A1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. iCAP-BI-28-KanR

    Plasmid

    #203532

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI28 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21a-LysRS-His

    Plasmid

    #124114

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Lysine-tRNA ligase (lysU Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PWPXLD-EGFR-WT

    Plasmid

    #133749

    Purpose

    Wild-type EGFR tagged with HA cloned into lentiviral PWPXLD plasimd

    Depositor

    Chay Kuo

    Article

    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

    Insert

    Wild type EGFR-HA (EGFR Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ABE8r-V106W

    Plasmid

    #208295

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-V106W-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLENTI6.3/AR-GC-E2325

    Plasmid

    #85128

    Purpose

    exogenous AR expression

    Depositor

    Karl-Henning Kalland

    Article

    Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

    Insert

    androgen receptor (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CAG FLEX BARK p2A mCherry

    Plasmid

    #117693

    Purpose

    CAG-FLEx-iBARK-p2A-mCherry: Viral expression vector for Cre-dependent Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs peptide
    

    Use

    Cre/Lox

    Tags

    HA / FLAG

    Expression

    Mutation

    Promoter

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. siRNA-resistant SigmaR1-mCherry

    Plasmid

    #226567

    Purpose

    Encodes siRNA resistant SigmaR1 protein labeled with mCherry

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SERPINE1-bio-His

    Plasmid

    #52077

    Purpose

    Expresses full-length Plasminogen activator inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINE1 (SERPINE1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-hsp27 wt FL

    Plasmid

    #17444

    Depositor

    Andrea Doseff

    Article

    Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

    Insert

    small heat shock protein27 (HSPB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDEST17-His10-ccdB

    Plasmid

    #166145

    Purpose

    Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSICO-CPSF6-eGFP

    Plasmid

    #167585

    Purpose

    Expresses human CPSF6 fused to eGFP

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    CPSF6-eGFP (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    WT

    Promoter

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Halo-KDEL

    Plasmid

    #124316

    Purpose

    Expresses ER specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Use

    Tags

    KDEL Seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 7i sgRNA for 4-μHOM reporter

    Plasmid

    #113626

    Purpose

    sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7i sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

35. pPW3758 : CMVd3-HsIRE1-HaloTag

    Plasmid

    #185680

    Purpose

    Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LentiV_Neo

    Plasmid

    #108101

    Purpose

    Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SPLICS Po-Mt Long P2A

    Plasmid

    #164115

    Purpose

    Detect the long Peroxisomes-mitochondria contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMTU-DO-URA

    Plasmid

    #160004

    Purpose

    Low-copy expression vector for K. marxianus, uracil selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    n/a

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hCas9_D10A

    Plasmid

    #41816

    Purpose

    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9_D10A
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A nickase

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV.GFAP.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209661

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95A.A119L

    Promoter

    GFAP

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge