We narrowed to 40,651 results for: tro

1. Mut2-RS

   Plasmid

   #182882

   Purpose

   A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

   Depositor

   Miriam Amiram

   Article

   Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

   Insert

   Mut2-RS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL.139

   Plasmid

   #184994

   Purpose

   Express Cas9-P2A-Eco1RT in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   SpCas9-P2A-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Human codon optimized RT

   Promoter

   TetOn-3g

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-GFAP104-ChR2-mCherry

   Plasmid

   #58892

   Purpose

   AAV-mediated expression of ChR2-mCherry under the GFAP promoter

   Depositor

   Edward Boyden

   Article

   Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

   Insert

   ChR2-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP104

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFS462

   Plasmid

   #89065

   Purpose

   his7 integration plasmid with Z3EVpr:GFP

   Depositor

   Nick Rhind

   Article

   Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

   Insert

   Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
   

   Use

   S. pombe his7 integration vector

   Tags

   Expression

   Mutation

   Promoter

   Z3EV

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-miniSOG-C1

   Plasmid

   #54801

   Purpose

   Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Type

   Empty backbone

   Use

   Tags

   mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pPbuCas13b

   Plasmid

   #184565

   Purpose

   Inducible expression of PbuCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Cas13b nuclease from Prevotella buccae
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS NL4-3 envFS eGFP PR-D25A

   Plasmid

   #101334

   Purpose

   pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 envFS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Protease D25A

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFAP-Lyn-mCherry-pHluorin

   Plasmid

   #187980

   Purpose

   Membrane targeted cytoplasmic fluorescent pH sensor

   Depositor

   Chris Dulla

   Article

   Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

   Insert

   Lyn-mcherry-pHluorin
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET51b(+)_LAMA-F98 (for GFP)

   Plasmid

   #130714

   Purpose

   Plasmid encoding the bacterial expression of LAMA-F98 for GFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   LAMA-F98
   

   Use

   Tags

   Hisx10

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDAS12124_PEAR-GFP-preedited

    Plasmid

    #177179

    Purpose

    PEAR-GFP control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. RP hk339-GFP

    Plasmid

    #24431

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin WT (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    monomeric kinesin

    Promoter

    Available since

    June 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-CoilR-Cys

    Plasmid

    #181989

    Purpose

    Used to label proteins with the CoilE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    CoilR-Cys
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. EK0303 SFFV-mCherry-SG(Bdnf.s3)SG-EGFP (FLP-IN)

    Plasmid

    #191180

    Purpose

    Negative control sensor in human cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s3:EGFP (negative control)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEM705 Gso (Ser)

    Plasmid

    #109353

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXWJ109Hg-gfp

    Plasmid

    #123148

    Purpose

    A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCLC3sHA

    Plasmid

    #52424

    Purpose

    mammalian expression of HA tagged rat CLC3 short transcript

    Depositor

    Steven Weinman

    Article

    Wang et al Gastroenterology. 2004 Apr;126(4):1157-6

    Insert

    CLC3 (Clcn3 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-K-I-N-CAAX

    Plasmid

    #41640

    Purpose

    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145K-IntersectinDH-Dronpa145N-CAAX
    

    Use

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    K145N on Dronpa

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b-17X-PTDH

    Plasmid

    #166786

    Purpose

    Phosphite dehydrogenase for NAD(P)H regeneration

    Depositor

    Wilfred van der Donk

    Article

    Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

    Insert

    Phosphite dehydroenase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A196R, T201S, A328T, E352N, and C356D- please see…

    Promoter

    T7

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-C1-CAMKIIalpha-T286A

    Plasmid

    #21220

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CAMKIIalpha-T286A (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T286A (Lab plasmid KS 0003).

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Integrin beta1(1-455)-tev-BHCys

    Plasmid

    #27315

    Depositor

    Timothy Springer

    Article

    Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

    Insert

    integrin beta1 (ITGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    beta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6

    Promoter

    Available since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

22. As4

    Plasmid

    #123161

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J115-30arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J115-30arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J115, ParsR

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC18-H1 RNAi

    Plasmid

    #87355

    Purpose

    RNAi expression vector based on H1 promoter and pUC18 vector backbone

    Depositor

    Saroj Mathupala

    Article

    Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

    Insert

    H1 RNA Promoter from U87MG glioma cells
    

    Use

    RNAi; Shrna expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 RNA

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-(SUMO)1

    Plasmid

    #127089

    Purpose

    Bacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    EGFP human SUMO3 (SUMO3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBD-pZ-FLAG

    Plasmid

    #183148

    Purpose

    Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 BD
    

    Use

    Yeast two-hybrid

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPIDNB

    Plasmid

    #169901

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Aivian expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PCTAIRE-1-HA

    Plasmid

    #1999

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    PCTAIRE-1 (CDK16 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pGEX-6p-1 Sesn2

    Plasmid

    #61873

    Purpose

    bacterial expression of a GST-Sesn2 (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GWF058

    Plasmid

    #133609

    Purpose

    UAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPR

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    Gal4DBD-NS3a, DNCR2-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pENTR R4-vas2-integrase-R3

    Plasmid

    #62299

    Purpose

    phageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoter

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Insert

    phageC31 integrase under control of vasa promoter and terminator
    

    Use

    Tags

    HA and SV40 NLS

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBabe-MITF

    Plasmid

    #64888

    Purpose

    Retroviral expression of murine MITF

    Depositor

    Ivana de la Serna

    Article

    de la Serna et al J Biol Chem. 2006 Jul 21;281(29):20233-4

    Insert

    MITF (Mitf Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pMN-285

    Plasmid

    #195472

    Purpose

    Lentiviral Integratable Inducible CD19CAR using synZiFTR ZF10

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    8X ZF10 BS - ybTATA - CD19 CAR - mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSR49

    Plasmid

    #69259

    Purpose

    phlh-8::tagBFP2

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    TagBFP2
    

    Use

    Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0, TagBFP2 contains 2 sing…

    Promoter

    hlh-8

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. BII-Sh-PnGW

    Plasmid

    #133359

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCS2+_Flag-myc-NES-Tir1

    Plasmid

    #117717

    Purpose

    mRNA synthesis of Flag-myc-NES-TIR1 for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    Flag-Myc-NES

    Expression

    Mammalian

    Mutation

    codon optimized TIR1 for murine expression

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PXWABS62(E11-TEV)

    Plasmid

    #123157

    Purpose

    For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ParsR-ABS62, Pecf11

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCOLA-Gent-EM7-Erv1p

    Plasmid

    #202484

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEGFP-N3-zz-p15_O

    Plasmid

    #147110

    Purpose

    Mammalian Expression of zz-p15

    Depositor

    Elisa Izaurralde

    Article

    Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

    Insert

    zz-p15 (NXT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCu-MCP-2yeGFP(405)

    Plasmid

    #121419

    Purpose

    Expressing MCP-2yeGFP

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

    Insert

    CUP1p-MCP-2yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge