We narrowed to 18,127 results for: Mut

1. pBB14

   Plasmid

   #18657

   Depositor

   Lynn Enquist

   Article

   Brideau et al J Virol. 1998 Jun . 72(6):4560-70.

   Insert

   Us9-EGFP gene fusion
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   First 106 amino acids of Us9 are intact. The stop…

   Promoter

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB1-sfGFP

   Plasmid

   #103983

   Purpose

   Plasmid encoding sfGFP

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   sfGFP
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S30R, Y39N, …

   Promoter

   proC

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5HT6-CFP-Venus(H148G)

   Plasmid

   #47501

   Purpose

   Fluorescent reporter for pH inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   Venus(H148G)
   

   Use

   Tags

   5HT6 and CFP

   Expression

   Mammalian

   Mutation

   H148G mutation

   Promoter

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pJK_proK1_eGFP

   Plasmid

   #59853

   Purpose

   Constitutively expresses eGFP with proK1 promoter

   Depositor

   Timothy Whitehead

   Article

   Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   S2R,S65G,S72A

   Promoter

   proK1

   Available Since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-U6-AksgRNA

   Plasmid

   #121955

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AkCas12b single chimeric gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bongwoori-R3

   Plasmid

   #101658

   Purpose

   A variant of Bongwoori with H237R mutation in its linker region. (* super ecliptic pHluorin A227D)

   Depositor

   Bradley Baker

   Article

   Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

   Insert

   Bongwoori-R3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. NFkBm2

   Plasmid

   #15090

   Depositor

   David Baltimore

   Article

   Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   miR-146a promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2 NFkB binding sites mutated

   Promoter

   Available Since

   June 6, 2007

   Availability

   Academic Institutions and Nonprofits only

8. LV anti-FITC-LaG16-R103A-T2A-BFP

   Plasmid

   #194935

   Purpose

   LV construct encoding R103A mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-myc-PICK1-rescue

   Plasmid

   #72573

   Purpose

   Expresses myc-PICK1 shRNA resistant cDNA

   Depositor

   Richard Huganir, Victor Anggono

   Article

   Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

   Insert

   PICK1 (Pick1 Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   5 silent mutations around shRNA recognition seque…

   Promoter

   CMV

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL690

    Plasmid

    #47753

    Purpose

    Expresses dCas9-VP64 fusion

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUGW-APPIce-T2A-mCherry

    Plasmid

    #190806

    Purpose

    Express Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Icelandic (A673T)

    Promoter

    Ubi C

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ155

    Plasmid

    #69306

    Purpose

    Gateway entry vector with AteCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    AteCas9D10A (Arabidopsis codon optimized)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A mutation; NLS fusion

    Promoter

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pegRNA_LMNA-1

    Plasmid

    #199273

    Purpose

    pegRNA used to induce LMNA (c.1824C > T) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LMNA 1824CtoT pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQF-mNGsacB

    Plasmid

    #203673

    Purpose

    pQF with mNeonGreen and sacB under control of PQ5

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Inserts

    mNeonGreen
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Has synonymous (silent) mutations to remove BsrGI…

    Promoter

    PQ5

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. YIp128-P30-His-pol30(K164R)

    Plasmid

    #99544

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K164R

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-U6-AasgRNA

    Plasmid

    #121954

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5_tAKARα-T391A

    Plasmid

    #119920

    Purpose

    Mammalian expression of the tAKARα sensor with T391A mutation

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα-T391A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC-DEST-R1R2

    Plasmid

    #141056

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMBP-LbCas12a H759A

    Plasmid

    #113518

    Purpose

    Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    H759A

    Promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-U6-AasgRNA3.8

    Plasmid

    #121958

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA, short version
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mCherry-FRBx2

    Plasmid

    #193571

    Purpose

    Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    mCherry
    

    Use

    Tags

    FRB (T2098L)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. YIplac211-Erg11-iGFPx3

    Plasmid

    #115422

    Purpose

    Express Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    Erg11-iGFPx3
    

    Use

    Tags

    iGFP

    Expression

    Yeast

    Mutation

    Promoter

    Endogenous

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BRAF - A12G in pENTR

    Plasmid

    #216606

    Purpose

    gateway cloning donor vector containing BRAF ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    A12G

    Promoter

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HOXA9-MSCV-full-GFP

    Plasmid

    #20977

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

    Insert

    HOXA9 (Hoxa9 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Full length cDNA with ~1.1kb 3'UTR with 2 pu…

    Promoter

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PKCalpha.K/N.FLAG

    Plasmid

    #10806

    Depositor

    Alex Toker

    Article

    Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

    Insert

    PKC alpha (PRKCA Bovine)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Kinase dead mutant: K368N (Lys 368 to Asn)

    Promoter

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

26. pReporter_2

    Plasmid

    #60563

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMD19T-slr0230-PL22-sgRNANT1-KmR

    Plasmid

    #73224

    Purpose

    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    PL22-sgRNA NT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PL22

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUC-DEST-R3R4(R)

    Plasmid

    #141010

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HA-RNF126 C231A

    Plasmid

    #138648

    Purpose

    Mammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)

    Depositor

    Jane McGlade

    Article

    Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

    Insert

    RNF126 (RNF126 Mouse)
    

    Use

    Tags

    HA-HA

    Expression

    Mammalian

    Mutation

    C231A

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pICSL50015

    Plasmid

    #50318

    Purpose

    Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mNEON (Branchistoma lanceolatum)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-EPN-01(ΔL1)

    Plasmid

    #80049

    Purpose

    Expression of EPN-01 mutated in TSG101 binding site.

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01(deltaPTAP)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PTAP10AAAA

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR-P5P4

    Plasmid

    #141064

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-FRB-LplA(W37V)

    Plasmid

    #35608

    Depositor

    Alice Ting

    Article

    Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

    Insert

    FRB-LplA(W37V)
    

    Use

    Tags

    FLAG (internal fusion), HA, and His Tag

    Expression

    Mammalian

    Mutation

    LplA has Trp37 to Val mutation

    Promoter

    CMV

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-Flag-YOD1 C160S

    Plasmid

    #49785

    Purpose

    Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

    Insert

    YOD1 (YOD1 Human)
    

    Use

    Tags

    Flag-tag

    Expression

    Mutation

    C160S

    Promoter

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pegRNA_LRRK2-3

    Plasmid

    #199272

    Purpose

    pegRNA used to induce LRRK2 (c. 6055 G > A) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LRRK2 6055GtoA pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBlue-TAL

    Plasmid

    #49401

    Purpose

    The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

    Depositor

    Michal Zurovec

    Article

    Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGPΩTp

    Plasmid

    #130660

    Purpose

    Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.

    Depositor

    Miguel Valvano

    Article

    Flannagan et al Infect Immun. 2007 Apr;75(4):1679-89. Ep

    Insert

    Ω trimethoprim cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Addition of Ω sites next to trimethoprim cassette…

    Promoter

    dhfr promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. JGE301_pAAV-hSyn-DIO-Flag-hM3Dq-V2(2D)-ABIcs-mCherry

    Plasmid

    #107246

    Purpose

    hM3DREADD fused to ABIcs for abscisic acid mediated recruitment of PYLcs labelled proteins.

    Depositor

    Bryan Roth

    Article

    Roth et al bioRxiv 251769

    Insert

    hM3DREADD
    

    Use

    AAV

    Tags

    ABIcs, FLAG, and mCherry

    Expression

    Mutation

    T to D and S to D mutations in V2-tail

    Promoter

    hSYN

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Vh-HA-2xFRB

    Plasmid

    #20224

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFRB (MTOR Human)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    One copy of FRB has a T2098L mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

40. pReporter_7

    Plasmid

    #60567

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge