164,223 results

1. pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

   Plasmid

   #187588

   Purpose

   Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   NLS
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5-FLAG-ITFG2

   Plasmid

   #87044

   Purpose

   mammalian expression of ITFG2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   ITFG2 (ITFG2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUAS-V5-miniTurboID-NES

   Plasmid

   #116905

   Purpose

   Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

   Depositor

   Norbert Perrimon

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurboID
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT-HA-hM3D(Gq)

   Plasmid

   #45547

   Purpose

   Expresses hM3D (Gq) neuronal activator

   Depositor

   Bryan Roth

   Article

   Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

   Insert

   hM3D (Gq)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS313-PRP8

   Plasmid

   #89966

   Purpose

   complements prp8 genomic deletion

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   none

   Promoter

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 Flag-HIF1B (#930)

   Plasmid

   #99916

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

   Insert

   HIF1B (ARNT Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBOB-VE-cadherin-GFP

   Plasmid

   #118732

   Purpose

   To express VE-cadherin tagged with GFP on C-terminus

   Depositor

   John Cooper

   Article

   Kim et al Mol Biol Cell. 2018 Jul 15;29(13):1693-1

   Insert

   Cadherin 5 (CD144) (CDH5 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   I503T (Common SNP-see comments)

   Promoter

   CMV

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBT1-proD-mCherry

   Plasmid

   #65823

   Purpose

   Const. mCherry expression , proD promoter, E. coli Expression

   Depositor

   Michael Lynch

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proD E . coli Constitutive Promoter

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT

   Plasmid

   #204694

   Purpose

   This plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   direct fusion of BRD4 and NUT

   Promoter

   CMV

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pemiRFP703-N1

    Plasmid

    #136558

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP703
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFAM-SNAPf

    Plasmid

    #187909

    Purpose

    Mammalian cell expression of SNAPf-labeled TFAM

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    SNAPf

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. OPEN-HLA-B*07:02-BirA

    Plasmid

    #239752

    Purpose

    E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-B*07:02 (HLA-B Human)
    

    Use

    Tags

    BirA substrate peptide

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    T7

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TRBV6-4

    Plasmid

    #237916

    Purpose

    Encodes TRBV6-4 allele and TRAC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Insert

    TRBV6-4-TRAC (TRBV6-4 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-mito-GreenPy1High

    Plasmid

    #239070

    Purpose

    Mammalian expression of mitochondria-targeted high affinity green fluorescent pyruvate biosensor GreenPy1High

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1High
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-mito-GreenPy1Lowest

    Plasmid

    #239072

    Purpose

    Mammalian expression of mitochondria-targeted lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Lowest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD-GreenPy1Highest

    Plasmid

    #239049

    Purpose

    Bacterial expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Highest
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-mito-GreenPy1Low

    Plasmid

    #239071

    Purpose

    Mammalian expression of mitochondria-targeted low affinity green fluorescent pyruvate biosensor GreenPy1Low

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Low
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-GreenPy1High

    Plasmid

    #239060

    Purpose

    Mammalian expression of high affinity green fluorescent pyruvate biosensor GreenPy1High

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1High
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-GreenPy1Low

    Plasmid

    #239061

    Purpose

    Mammalian expression of low affinity green fluorescent pyruvate biosensor GreenPy1Low

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Low
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-dGreenPy1

    Plasmid

    #239063

    Purpose

    Mammalian expression of green fluorescent pyruvate control biosensor dGreenPy1

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    dGreenPy1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-mito-dGreenPy1

    Plasmid

    #239073

    Purpose

    Mammalian expression of mitochondria-targeted green fluorescent pyruvate control biosensor dGreenPy1

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    dGreenPy1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBAD-GreenPy1High

    Plasmid

    #239050

    Purpose

    Bacterial expression of high affinity green fluorescent pyruvate biosensor GreenPy1High

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1High
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-GreenPy1Lowest

    Plasmid

    #239062

    Purpose

    Mammalian expression of lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Lowest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBAD-GreenPy1Lowest

    Plasmid

    #239052

    Purpose

    Bacterial expression of lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Lowest
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TRBV27

    Plasmid

    #237947

    Purpose

    Encodes TRBV27 allele and TRAC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Insert

    TRBV27-TRAC (TRBV27 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRAV19

    Plasmid

    #237976

    Purpose

    Encodes TRAV19 allele, P2A, and TRBC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Insert

    TRBC-P2A-TRAV19 (TRAV19 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TRAV12-2

    Plasmid

    #237968

    Purpose

    Encodes TRAV12-2 allele, P2A, and TRBC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Insert

    TRBC-P2A-TRAV12-2 (TRAV12-2 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-TMEM30A-V5

    Plasmid

    #225510

    Purpose

    TMEM30A expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    TMEM30A (TMEM30A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBOBI-hPLD3-flag

    Plasmid

    #222640

    Purpose

    lentiviral vector for full length human PLD3 expression (with flag tag)

    Depositor

    David Nemazee

    Insert

    PLD3 (PLD3 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. OPEN-A*24:02

    Plasmid

    #235133

    Purpose

    E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*24:02 (HLA-A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. OPEN-HLA-A*01:01

    Plasmid

    #235134

    Purpose

    E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. OPEN-HLA-A*11:01-BirA

    Plasmid

    #235135

    Purpose

    E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*11:01 (HLA-A Human)
    

    Use

    Tags

    BirA

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLV_CAG_Gag-EIF4E

    Plasmid

    #212635

    Purpose

    Lentivirus vector to express an MLV Gag-EIF4E fusion protein

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Lentiviral

    Tags

    EIF4E

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV_CAG_Gag

    Plasmid

    #212634

    Purpose

    Lentivirus vector to express MLV Gag

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLV_CAG_Gag∆MA

    Plasmid

    #212633

    Purpose

    Lentivirus vector to express MLV Gag with truncated MA domain

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of MA domain (amino acids 2-215)

    Promoter

    CAG

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV_CMV_HA-VSVG

    Plasmid

    #212645

    Purpose

    Lentivirus vector to express HA-VSVG

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    VSVG
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV_CMV_FLAG-VSVG

    Plasmid

    #212642

    Purpose

    Lentivirus vector to express FLAG-VSVG

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    VSVG
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO GABARAP

    Plasmid

    #218427

    Purpose

    Expression of tagless GABARAP

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJS119

    Plasmid

    #207592

    Purpose

    GFP-TonB deltaII expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TonB deltaII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJS104

    Plasmid

    #207589

    Purpose

    GFP-TolA deltaII expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TolA deltaII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge