161,570 results

1. pHAGE-TGFBR2

   Plasmid

   #116800

   Purpose

   Lentiviral expression of TGFBR2

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   TGFBR2 (TGFBR2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C3-Exo70

   Plasmid

   #53761

   Purpose

   pEGFP-C3 plasmid that expresses Exo70

   Depositor

   Channing Der

   Article

   Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

   Insert

   Exo70 (EXOC7 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-CR029

   Plasmid

   #234041

   Purpose

   expresses evoCDA1 BE4max base editor in a human T cell optimized lentiviral transfer plasmid

   Depositor

   Alexander Marson

   Article

   Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

   Insert

   evoCDA1-Cas9(D10A)-P2A-blast
   

   Use

   Lentiviral

   Tags

   blast

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CR102

   Plasmid

   #234043

   Purpose

   expresses ABE8e(V106W) - SpG Cas9 base editor in a human T cell optimized lentiviral transfer plasmid

   Depositor

   Alexander Marson

   Article

   Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

   Insert

   TadA8e(V106W)-SpG-Cas9(D10A)-P2A-blast
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21a-LeuRS-His

   Plasmid

   #124113

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Leucyl-tRNA ligase (leuS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAKlux2

   Plasmid

   #14080

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac, T3

   Available since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. Strep-His-Tev-hCGN

   Plasmid

   #236512

   Purpose

   For expression of Cingulin (human) in insect cells

   Depositor

   Sandra Citi

   Article

   Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

   Insert

   CGN (CGN Human)
   

   Use

   Tags

   2xStrep-10xHis-Tev

   Expression

   Insect

   Mutation

   WT

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP15285 - pAAV-AiE0170h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN5285)

   Plasmid

   #230509

   Purpose

   AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

   Plasmid

   #231364

   Purpose

   Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAIP1

    Plasmid

    #233685

    Purpose

    Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.

    Depositor

    Christopher Bahl

    Insert

    Ulp1-like SUMO protease
    

    Use

    Tags

    CAHS-107838 and HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. SJZ5-HPH

    Plasmid

    #228258

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    hph
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SSA1 S.c.

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR/zeo-MACROD1

    Plasmid

    #172572

    Purpose

    For gateway cloning of N-terminal tagged MACROD1 constructs.

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_RTN3E-mScarlet3

    Plasmid

    #216255

    Purpose

    mammalian expression vector for RTN3E-mScarlet3

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    reticulon 3 isoform e (RTN3 Human)
    

    Use

    Tags

    mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-C34-H

    Plasmid

    #223966

    Purpose

    Anti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.

    Depositor

    Dmitriy Mazurov

    Article

    Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

    Insert

    Variable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPOTv7-blast-mStayGold[E138D]

    Plasmid

    #221049

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mStayGold[E138D]
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid

    #215769

    Purpose

    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

    Depositor

    Dimitrios Wagner

    Article

    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

    Inserts

    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Human, Mouse, Bovine)
    TRAC-3' homology arm (TRAC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cas12a PAM mutated

    Promoter

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AA304

    Plasmid

    #216072

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUPD2-HR1bR181A R185A-C (B3-B4)

    Plasmid

    #211855

    Purpose

    CDS for mutant HR1b degron in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    R181A R185A

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)

    Plasmid

    #211861

    Purpose

    Nuclear-localized E3-DART ligase in domestication vector.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    3xNLS-HA

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF0406

    Plasmid

    #143023

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CTCFL (CTCFL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156982

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PTGFRN (PTGFRN Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR/zeo-MACROD2

    Plasmid

    #172573

    Purpose

    For gateway cloning of N-terminal tagged MACROD2 constructs.

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD2 (MACROD2 Human)
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1 SARS-CoV-2 E

    Plasmid

    #158080

    Purpose

    Expresses SARS-CoV-2 envelope protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 envelope (E )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GST-thr-RAF1-RBD

    Plasmid

    #86033

    Purpose

    Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation

    Depositor

    Dominic Esposito

    Insert

    RAF1 RBD (Ras-binding domain) (RAF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains residues 1-149

    Promoter

    T7

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSEED-mGreenLantern:ALFA

    Plasmid

    #219644

    Purpose

    Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mGreenLantern:ALFA SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lck-GFP

    Plasmid

    #61099

    Purpose

    Expression of a membrane-targeted form of eGFP

    Depositor

    Steven Green

    Article

    Benediktsson et al J Neurosci Methods. 2005 Jan 30;141(1):4

    Insert

    Lck-GFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GW1-Peredox-mCitrine

    Plasmid

    #32386

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCitrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pWM_12x601_30bpLinker

    Plasmid

    #157789

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_30bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1(-)-qmTQ2-cAMP

    Plasmid

    #225250

    Purpose

    mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEvol-MjaYRS

    Plasmid

    #153557

    Purpose

    Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli

    Depositor

    Huiwang Ai

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

    Inserts

    MJaYRS (first copy)
    MJaYRS (second copy)
    amber suppression tRNA under proK promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    glnS and pBAD

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEGFP-hsRPA70 (MSW#473)

    Plasmid

    #208067

    Purpose

    Expression of GFP-tagged hsRPA1 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA1 (RPA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pALPS vif

    Plasmid

    #101328

    Purpose

    Encodes codon optimized NL4-3 vif

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 vif
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTrcAgBIS (CO)

    Plasmid

    #35153

    Depositor

    Taek Soon Lee, Jay Keasling

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    Bisabolene Synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 2xP53_RE::FLuc

    Plasmid

    #178316

    Purpose

    P53 Firefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    P53 FLuc Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMSCVpuro-Cas9-EGFP

    Plasmid

    #159126

    Purpose

    Expresses active Cas9 fused with EGFP for fluorescence microscopy

    Depositor

    Taekjip Ha

    Article

    Liu et al Science. 2020 Jun 12;368(6496):1265-1269

    Insert

    Cas9 fused to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR promoter

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA4-Beclin1 (FL)

    Plasmid

    #24388

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBabe.puro.CDK8.flag

    Plasmid

    #19758

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pYTK095

    Plasmid

    #65202

    Purpose

    Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Douglas Kim, Loren Looger, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

    Plasmid

    #159697

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge