165,025 results

1. pRSFDuet1_CoiA1_CoiB

   Plasmid

   #208761

   Purpose

   Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiA1
   CoiB
   

   Tags

   Hisx6 Tag and SUMO

   Expression

   Bacterial

   Mutation

   Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3TP-lux

   Plasmid

   #11767

   Depositor

   Jeff Wrana, Joan Massague

   Article

   Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

   Insert

   3TP
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pSL21-VEX

   Plasmid

   #158230

   Purpose

   Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)

   Depositor

   Junwei Shi

   Article

   Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-TetOne-zeo_3xHA-TurboID-NLS

   Plasmid

   #200022

   Purpose

   For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   3xHA-TurboID-NLS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3Gs

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1α-DIO-CFL-SN

   Plasmid

   #181740

   Purpose

   AAV vector for expressing CFL-SN

   Depositor

   Yasunori Hayashi

   Article

   Goto et al Science. 2021 Nov 12;374(6569):857-863.

   Insert

   CFL-SN
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. myc-KIF5C

   Plasmid

   #127618

   Purpose

   N-terminal myc-tagged mouse KIF5C

   Depositor

   Josef Kittler

   Article

   Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

   Insert

   KIF5C (Kif5c Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-ChRmine-mScarlet-WPRE

   Plasmid

   #130994

   Purpose

   AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet

   Promoter

   hSyn

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATGL*-V5-APEX2

   Plasmid

   #170574

   Purpose

   A C-terminal APEX2 fusion to the human ATGL S47A protein

   Depositor

   James Olzmann

   Article

   Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

   Insert

   ATGL* (PNPLA2 Human)
   

   Use

   Lentiviral

   Tags

   V5-APEX2

   Expression

   Mammalian

   Mutation

   changed serine 47 to alanine

   Promoter

   CMV

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDM069

   Plasmid

   #216821

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold2
   

   Tags

   RIPGLIN and VinnyLinker

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGCS-N3(3xFLAG)

    Plasmid

    #85720

    Purpose

    Gateway destination vector

    Depositor

    Hai-Ning Du

    Article

    Wang et al Acta Biochim Biophys Sin (Shanghai). 201

    Type

    Empty backbone

    Use

    Mammalian/avian/xenopus/zebrafish

    Tags

    3xFLAG

    Available Since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-PITCh-GOLGA2

    Plasmid

    #207791

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-SYN-mBaoJin-oPRE

    Plasmid

    #218683

    Purpose

    AAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

    Depositor

    Bart Nieuwenhuis

    Insert

    mBaoJin
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHDM-VSV-G

    Plasmid

    #164440

    Purpose

    Encodes VSV-G protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    VSV-G
    

    Expression

    Mammalian

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-PTRE-tight-hM3Dq-mCherry

    Plasmid

    #66795

    Purpose

    Expresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging

    Depositor

    William Wisden

    Article

    Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

    Insert

    pTIGHT promoter-hM3Dq-mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    pTIGHT TRE

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-ULK2

    Plasmid

    #97075

    Purpose

    Expresses ULK2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    ULK2 (ULK2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS2-GFP

    Plasmid

    #27121

    Depositor

    Robert Singer

    Article

    Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

    Insert

    MS2
    

    Tags

    EGFP, HA, and SV40 NLS

    Expression

    Mammalian

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCHW101020

    Plasmid

    #222454

    Purpose

    Level 1, Position 2 containing 35S::CUP2-p65::NOS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    35S::CUP2-p65::NOS (CUP2 )
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    no

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131461

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRN111

    Plasmid

    #84463

    Purpose

    pJB38-NWMN29-30 + SarA_P1-mCherry-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-mCherry from pRN11
    

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-SENP2

    Plasmid

    #18047

    Depositor

    Edward Yeh

    Article

    Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

    Insert

    SENP2 (SENP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET His6 Sumo TEV LIC cloning vector (2S-T)

    Plasmid

    #29711

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, SUMO, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV.hSynap.ArcLightD.WPRE.SV40

    Plasmid

    #100037

    Purpose

    AAV expression of ArcLightD driven by hSynap promoter

    Depositor

    Vincent Pieribone

    Insert

    ArcLightD
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynap

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGL2- Empty control -SV40-Luc

    Plasmid

    #26280

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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24. P4-TadCBEd

    Plasmid

    #225143

    Purpose

    Packaging plasmid for TadCBEd in v3b PE-eVLP architecture

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    P4-TadCBEd
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-hsyn-GRAB_Ado1.0

    Plasmid

    #153285

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in neurons

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. Tom20-V5-FKBP-AP_pLX304

    Plasmid

    #120914

    Purpose

    Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    Tom20-V5-FKBP-AP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. BPK2014-AaCas12b

    Plasmid

    #121949

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Tags

    10xHis tag

    Expression

    Bacterial

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. AAV hSYN DIO mGluR1-myc-FLAG

    Plasmid

    #166227

    Purpose

    AAV expressing cre-dependent mGluR1-myc-FLAG

    Depositor

    Jason Aoto

    Article

    Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

    Insert

    metabotropic glutamate receptor 1 (Grm1 Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc and FLAG

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDV06.2c_DART-VADAR_iRFP720-sensor

    Plasmid

    #200410

    Purpose

    pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor detecting iRFP720
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

    Plasmid

    #209077

    Purpose

    Ngn2 mediated cortical neuron induction, dox induction is included

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    NGN2 (Neurog2 Mouse)
    SNAPtag
    

    Expression

    Mammalian

    Promoter

    TRE4G

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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31. Lenti-EF1alpha-dCas9-p300_Blast

    Plasmid

    #192653

    Purpose

    3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSD141B

    Plasmid

    #218139

    Purpose

    Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD56-eGFP-IRES2-mCherry
    

    Use

    Lentiviral

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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33. AAV-Ef1a-InteinC-CreC

    Plasmid

    #187615

    Purpose

    AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

    Depositor

    Il Hwan Kim

    Article

    Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

    Insert

    InteinC-CreC
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRsetB-His7tag-Peredox-mCherry

    Plasmid

    #32382

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    His7-Peredox-mCherry
    

    Tags

    His7tag

    Expression

    Bacterial

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid

    #122846

    Purpose

    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    human FASN/ human fatty acid synthase (FASN Human)
    

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. eGFP STAT1 S727E

    Plasmid

    #12305

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

    Insert

    STAT1 S727E (STAT1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S727E

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Snap-Omp25

    Plasmid

    #69599

    Purpose

    Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    Snap-Omp25
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mCherry-Vav2

    Plasmid

    #129623

    Purpose

    RhoGEF

    Depositor

    Dorus Gadella

    Article

    Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

    Insert

    Vav2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-SMVP-Cas9C

    Plasmid

    #80931

    Purpose

    Expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDY1622 NOLC1 sgRNA 1

    Plasmid

    #234833

    Purpose

    sgRNA 1 for NOLC1 STITCHR insertion

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge