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Plasmid#203442PurposePlasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activityDepositorInsertRfxCas13d-T2A-mCherry
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC30A6
Plasmid#132065PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC30A6 (SLC30A6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS395_PhCMV-SB100
Plasmid#169633PurposeExpression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)DepositorInsertPCMV-driven SB100
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti GFP-AMOT p130
Plasmid#32828DepositorInsertAMOT p130 (AMOT Human)
UseLentiviralTagsGFP hAMOTExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFBP_promoter_acceptor
Plasmid#189920PurposeBinary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132A
Plasmid#69274PurposeGolden Gate entry vector; 2nd gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV9cap
Plasmid#198016PurposeFor high-level expression of the AAV9 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV9 cap
UseAAVTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GFP10-ZIP
Plasmid#182241PurposeTripartite split-GFP mammalian. Express leucine zipper with GFP10DepositorInsertGFP10-ZIP
UseTagssplit-GFP10 tripartiteExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-CD4-sTb(C)-M13-GFP
Plasmid#219784PurposesTb(C) half of CaST for neuron expressionDepositorInsertCD4-sTb(C)-M13-GFP
UseTagsHAExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
Plasmid#63169PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporterDepositorInsert"spaghetti monster GFP-FLAG"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgSLC7A11/xCT-1
Plasmid#161818Purposeknock out SLC7A11/xCT in mammalian cellsDepositorInsertSLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2
Plasmid#191338PurposeExpresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice culturesDepositorInsertTUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE
Plasmid#166970PurposeCre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.DepositorInsertHomo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
UseAAV and Cre/LoxTagsFLAG, T2A, and mCherryExpressionMammalianMutationPromoterhsynAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMasterK
Plasmid#165081PurposeEpisomal expression of reprogramming factors.DepositorInsertsUseEpisomalTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA
Plasmid#164615PurposeExpresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.DepositorInsertRenilla luciferase
UseTags24xMS2v5 (3'UTR) and SunTagExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-mRFP-gsk3 s9a
Plasmid#24371DepositorInsertGSK3beta(S9A) (GSK3B Human)
UseAdenoviralTagsmRFPExpressionMammalianMutationS9A. Mutant version of GSK3 that lacks the inhibi…PromoterAvailable sinceMay 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CMV-GFP-BGHpA
Plasmid#190239PurposeExpresses EGFP in mammalian cellsDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
mKikGR-N1
Plasmid#54756PurposeLocalization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593DepositorTypeEmpty backboneUseTagsmKikGRExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28CLY2
Plasmid#21761DepositorInsertECFP -EYFP linked by flexible linker containing two SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pDD287 (mKate2^SEC^3xMyc
Plasmid#70685PurposemKate2^SEC^3xMyc vector with ccdB sites for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized mKate2ExpressionWormMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G12C
Plasmid#58901PurposeExpresses human Kras with a G12C mutationDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationG12C mutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Clover2-N1
Plasmid#54537PurposeLocalization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.DepositorTypeEmpty backboneUseTagsClover2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHW110032
Plasmid#222438PurposeLevel 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters.DepositorInsertCBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW00011
Plasmid#222439PurposeLevel 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.DepositorInsertCUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-CalfluxVTN
Plasmid#83926PurposeBioluminescent Reporter of Intracellular CalciumDepositorInsertCalfluxVTN
UseTagsExpressionBacterialMutationNonePromoterT7Available sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-BAZ1B
Plasmid#65372PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertBAZ1B (BAZ1B Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+12)
Plasmid#199161Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+12)
UseTagsMGHHHHHHGGAExpressionMutationE16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Hygro DEST (693-2)
Plasmid#17291Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, HygroDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
Plasmid#196192Purposehuman synapsin promoter driven dTomato expression at human AAVS1 safe harbour locusDepositorInsert2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
UseZnf donorTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-sfGFP
Plasmid#125747PurposesfGFP without iCreERT2 fusionDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
Plasmid#172508PurposeEncodes the mature DisCas7-11 DR along with a golden gate site for spacer cloningDepositorInsertpU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-Q61L
Plasmid#12981DepositorInsertRac1 constitutively active (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationQ61L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLEX-NES-jGCaMP8m-WPRE
Plasmid#186045PurposeAAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expressionDepositorInsertNES-jGCaMP8m
UseAAVTagsNES-6xHisExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-N1-mouse-superfolderGFP (mGRASP)
Plasmid#42143DepositorInsertpCMV-N1-superfolderGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0123
Plasmid#141528PurposeLentiviral vector for overexpressing the OLIG2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertOLIG2 (OLIG2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZT-AAVS1-R1
Plasmid#52638PurposeTALEN-mediated gene editing at human PPP1R12C/AAVS1 locusDepositorInsertRight AAVS1 TALEN
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaZ
Plasmid#196053PurposeEncodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry- SEC61B
Plasmid#121160PurposeMammalian expression of mCherry tagged SEC61B, used as a general ER markerDepositorInsertSEC61B (SEC61B )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only