We narrowed to 125,704 results for: CIL

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M51 Super 8x FOPFlash (TOPFlash mutant)

Plasmid

#12457

Purpose

Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

mutant TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Expression

Mammalian

Mutation

mutant TCF/LEF binding sites

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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pRK793

Plasmid

#8827

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219V mutant

Tags

His, MBP (with TEV), and polyarginine

Expression

Bacterial

Mutation

S219V mutation (improves stability)

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pCW-Cas9

Plasmid

#50661

Purpose

Doxycycline-inducible lentiviral expression of SpCas9

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Tet ON

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

Plasmid

#61591

Purpose

A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Inserts

hSaCas9
Chimeric guide for SaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Available Since

Feb. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-ERT2CreERT2

Plasmid

#13777

Purpose

Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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lenti dCAS-VP64_Blast

Plasmid

#61425

Purpose

3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Lentiviral Prep

Insert

dCAS9(D10A, N863A)-VP64_2A_Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1A

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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hCas9

Plasmid

#41815

Purpose

Expresses human codon optimized Cas9 nuclease for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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HA-Ubiquitin

Plasmid

#18712

Purpose

Mammalian expression of human ubiquitin with HA tag

Depositor

Edward Yeh

Article

Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

Insert

ubiquitin (UBB Human)

Tags

HA

Expression

Mammalian

Mutation

The HA-Ubiquitin insert is from the UBB gene. The…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2

Plasmid

#132775

Purpose

Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCL-Eco

Plasmid

#12371

Depositor

Inder Verma

Article

Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

Insert

gag/pol/env

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0

Plasmid

#62987

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCW57.1

Plasmid

#41393

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-WT

Plasmid

#17608

Purpose

Mammalian expression of HA tagged ubiquitin

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV2/9n

Plasmid

#112865

Purpose

AAV packaging plasmid expressing Rep/Cap genes

Depositor

James M. Wilson

Insert

Rep2/Cap9

Use

AAV

Promoter

Truncated P5

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 APEX2-NES

Plasmid

#49386

Purpose

Expresses APEX2 in the cytosol of mammalian cells

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Flag - APEX2 - NES

Tags

Flag

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available Since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2GFP

Plasmid

#82416

Purpose

3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

GFP

Use

CRISPR and Lentiviral

Promoter

EFS (P2A)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-M2rtTA

Plasmid

#20342

Purpose

Lentiviral plasmid expressing the reverse tetracycline transactivator

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

M2rtTA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre

Plasmid

#13775

Purpose

Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre

Use

Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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SP-dCas9-VPR

Plasmid

#63798

Purpose

SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

SP-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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C. difficile toxin B mRNA toehold switch sensor

Plasmid

#110715

Purpose

Toehold switch sensor to detect the tcdB mRNA from toxigenic C. difficile with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

C. difficile toxin B toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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