We narrowed to 13,711 results for: Cas9

1. Cas9-GFP_sg_mTfeb

   Plasmid

   #79006

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   sgTfeb (Tfeb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. HiFi Cas9

   Plasmid

   #188490

   Purpose

   Expresses FLAG tagged HiFi Cas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R691A

   Promoter

   EF1a

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAct-Cas9

   Plasmid

   #62209

   Purpose

   Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpRY Cas9

   Plasmid

   #188491

   Purpose

   Expresses FLAG tagged SpRY Cas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX311-Cas9

   Plasmid

   #118018

   Purpose

   Constitutive expression of Cas9

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Adeno Cas9

   Plasmid

   #64072

   Purpose

   Adenoviral vector for the expression of the Cas9

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   U6 and CBh

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAEL005635-Cas9

   Plasmid

   #100608

   Purpose

   The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL005635-hspcas9-GFP
   Opie2-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Mutation

   Promoter

   AAEL005635 and Opie2

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAH01Sp::Cas9

   Plasmid

   #128160

   Purpose

   Ptet-controlled expression of S. pyogenes Cas9

   Depositor

   Calvin Henard

   Article

   Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

   Insert

   Sp Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHsp70-Cas9

   Plasmid

   #45945

   Purpose

   The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

   Depositor

   Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Expression

   Mutation

   Silent change from C to A at bp 834 of insert

   Promoter

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX2-Cas9

    Plasmid

    #85811

    Purpose

    Arabinose inducible Cas9 in a broad host range backbone.

    Depositor

    Ryan Gill

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas9 MDC123

    Plasmid

    #59184

    Purpose

    2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Glycine Max codon optimized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2X35S

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas9-sgRNA-A

    Plasmid

    #149369

    Purpose

    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, PGK, and U6

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cas9 Null

    Plasmid

    #198727

    Purpose

    Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pG10-90

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p201B Cas9

    Plasmid

    #59177

    Purpose

    Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    BAR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cas9-GFP_sg_mAMPKa1

    Plasmid

    #79004

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa1 (Prkaa1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pME-Cas9

    Plasmid

    #64237

    Purpose

    expresses codon optimized Cas9 in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas9-GFP_sg_mAMPKa2

    Plasmid

    #79005

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa2 (Prkaa2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. SETMAR-Cas9

    Plasmid

    #186701

    Purpose

    Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETMAR-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUB-Cas9

    Plasmid

    #86556

    Purpose

    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Cas9 is codon optimized for C. reinhardtii

    Promoter

    UBIQUITIN10

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PE-Cas9

    Plasmid

    #174851

    Purpose

    Express PE-Cas9 in mammalian cells

    Depositor

    Wen Xue

    Article

    Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    PE-Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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