162,717 results

1. HA-MBP-TEVpCS-ADGRG3 CTF-FLAG

   Plasmid

   #217701

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRG3 (ADGRG3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-249

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-MBP-TEVpCS-ADGRF1 CTFdeltaTA-FLAG

   Plasmid

   #217727

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRF1 (ADGRF1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-572

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA-MBP-TEVpCS-ADGRG4 CTFdeltaTA-FLAG

   Plasmid

   #217735

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRG4 (ADGRG4 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-2727

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HA-MBP-TEVpCS-ADGRG3 CTFdeltaTA-FLAG

   Plasmid

   #217734

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRG3 (ADGRG3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-255

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HA-MBP-TEVpCS-ADGRG7 CTFdeltaTA-FLAG

   Plasmid

   #217738

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRG7 (ADGRG7 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-421

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA-MBP-TEVpCS-ADGRG2 CTFdeltaTA-FLAG

   Plasmid

   #217733

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRG2 (ADGRG2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-612

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBiT3.1-C_RAE1:1-368

   Plasmid

   #211301

   Purpose

   Mammalian expression of full-length human RAE1. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   RAE1:M1-K368 (RAE1 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1646

   Plasmid

   #143531

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF423 (ZNF423 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF0597

   Plasmid

   #142655

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMARCB1 (SMARCB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD649-HAsp-MCAM-COMP5AP-AviTag-9xHis

    Plasmid

    #157473

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    MCAM (MCAM Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0684

    Plasmid

    #141661

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PRDM13 (PRDM13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MAC_N_ELK4

    Plasmid

    #187745

    Purpose

    MAC-tagged gene expression of human ELK4

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ELK4 (ELK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti_CMV:Flag-CeNL_SV40:PuroR

    Plasmid

    #183039

    Purpose

    Expresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    CeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti_CMV:Flag-YeNL_SV40:PuroR

    Plasmid

    #183041

    Purpose

    Expresses the FLAG-tagged YeNL (Yellow Nanolantern) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    YeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti_CMV:Flag-OeNL_SV40:PuroR

    Plasmid

    #183042

    Purpose

    Expresses the FLAG-tagged OeNL (Orange Nanolantern) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    OeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-ReNL

    Plasmid

    #123138

    Purpose

    Piggybac transposon plasmid for live animal optical imaging of Red-enhanced nanolantern

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Red-enhanced Nanolantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRDAX-VALENTIA-021 Lamp1-Sirius//BsaI

    Plasmid

    #130202

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    Lamp1-Sirius
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C1ORF43(1-41)-3xFLAG

    Plasmid

    #130881

    Purpose

    Expresses first 41 amino acids of human C1ORF43 with C-terminal 3xFLAG tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    C1ORF43 (C1orf43 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acids 44-253

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR1275

    Plasmid

    #111114

    Purpose

    Expresses RAD51C_+_56770199.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    RAD51C gRNA (RAD51C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-6P-1 p37deltaSEP

    Plasmid

    #113501

    Purpose

    Bacterial expression of GST-tagged p37 lacking the SEP domain

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 140-206

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTSara*SZ

    Plasmid

    #107957

    Purpose

    Arabinose-inducible split T7*RNAP with SynZIP domains 17/18

    Depositor

    Jim Haseloff

    Article

    Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

    Insert

    T7RNAP (modified)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    R632S

    Promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pML104

    Plasmid

    #67638

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid

    #218190

    Purpose

    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    His and NSP4 signal secretion peptide

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pU6-pegRNA-GG-acceptor

    Plasmid

    #132777

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Exchangeable cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #102796

    Purpose

    Retrovirus for delivery of one sgRNA (empty, bbsI)

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    U6 sgRNA
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHSE401

    Plasmid

    #62201

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

    Plasmid

    #207664

    Purpose

    Neuropeptide degradation

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    NEP (MME Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mRUBY3

    Expression

    Mutation

    Addition of signaling peptide (POMC) on the N-ter…

    Promoter

    hSynapsin

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLTR-RD114A

    Plasmid

    #17576

    Depositor

    Jakob Reiser

    Article

    Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

    Insert

    RD114 envelope glycoprotein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

    Plasmid

    #216490

    Purpose

    Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTight-9-124-BclxL

    Plasmid

    #60857

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTight

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge