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We narrowed to 7,425 results for: tet on

Showing: 1551 - 1600 of 7425 results
  1. pLKO-Tet-puro-hRAF1-shRNA-1

    Plasmid
    #185371
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1
    Depositor
    Insert
    RAF1 (RAF1 Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129028
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid
    #104413
    Purpose
    transient expression of dCas9-dTET1 fusion protein
    Depositor
    Insert
    dTET1
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H1672Y-D1674A
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pLKO-Tet-puro-hARAF-shRNA-1

    Plasmid
    #185370
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF
    Depositor
    Insert
    ARAF (ARAF Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. FLAG-FullLength-TET1-CXXC/LLAA

    Plasmid
    #124399
    Purpose
    Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells
    Depositor
    Insert
    FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    CXXC zinc finger deletion (aa 585-624) and L897A/…
    Available Since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. TET-pLKO-neo CXCR4-sh3

    Plasmid
    #163743
    Purpose
    Lentivirus for inducible knockdown of CXCR4 (human)
    Depositor
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. TET-pLKO-neo CXCR4-sh1

    Plasmid
    #163741
    Purpose
    Lentivirus for inducible knockdown of CXCR4 (human)
    Depositor
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. TET-pLKO-neo CXCR4-sh2

    Plasmid
    #163742
    Purpose
    Lentivirus for inducible knockdown of CXCR4 (human)
    Depositor
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pEnt R3L2 TetO(sh) mCh-Rs1-2

    Plasmid
    #24417
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Plasmid also known as pEntR3L2 TetO-mCh-Rs1.
    Depositor
    Insert
    Rs1
    Use
    Entry vector
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    short TetO, miniCMV
    Available Since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
  11. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

    Plasmid
    #121806
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa
    Insert
    HA epitope tag-polyA + EF1a::TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG

    Plasmid
    #129758
    Purpose
    Dox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells
    Depositor
    Insert
    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 36 to Alanine and Leucine 3…
    Promoter
    TRE3Gs and Tet inducible promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pFA6a-kanMX-tetO-Pcyc1-LINKER

    Plasmid
    #52685
    Purpose
    Same as 41023, with additional linker
    Depositor
    Insert
    tetO7-Pcyc1+LINKER
    Use
    Promoter replacement
    Expression
    Yeast
    Available Since
    May 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  14. pLVX-TetOne-bleo-atpD-K155Q

    Plasmid
    #248068
    Purpose
    Lentiviral expression of a catalytically inactive mutant of the beta subunit of ATPGobble. Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for a LOF control.
    Depositor
    Insert
    atpD
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    Lysine 155 to Glutamine, codon-optimized for expr…
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 1551 - 1600 of 7425 results