We narrowed to 15,833 results for: grna

1. nontarget S. pyogenes scaffold CARGO array

   Plasmid

   #191319

   Purpose

   Contains an array of nontargeting guide RNAs

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX330A_dCas9-1x4

   Plasmid

   #63598

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LentiGuide-TagBFP-2A-BSD

   Plasmid

   #167929

   Purpose

   Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCFD3-NS

   Plasmid

   #149545

   Purpose

   Expression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYPQ144

   Plasmid

   #69296

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pG3

   Plasmid

   #162604

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.1-puro-shFAM134B

   Plasmid

   #109013

   Purpose

   short-hairpin knockdown of targeted gene

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   shFAM134B (RETREG1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1-TSC2

   Plasmid

   #15478

   Depositor

   Do-Hyung Kim

   Article

   Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

   Insert

   TSC2 shRNA (TSC2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSWAP_Entry_T2

   Plasmid

   #184864

   Purpose

   pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pSWAP_Entry_T1

    Plasmid

    #184863

    Purpose

    pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pAAV-SEP-GluA1 TKIT

    Plasmid

    #169441

    Purpose

    Expression of 2 guides + donor DNA

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMLS597

    Plasmid

    #188884

    Purpose

    dpy-10 sgRNA expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    PU6(K09B11.12)::dpy-10 sgRNA (dpy-10 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSP618

    Plasmid

    #169235

    Purpose

    Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

    Depositor

    William Greenleaf

    Article

    Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LRT2B-AdGO2

    Plasmid

    #136911

    Purpose

    Lentivirus expressing AdGO2 sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgAdGO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    hU6

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ141A

    Plasmid

    #69290

    Purpose

    Gateway entry vector; single gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJAT30

    Plasmid

    #204294

    Purpose

    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR-dCas9

    Plasmid

    #125687

    Purpose

    Gene knockdown for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spdCas9, sgRNA casette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX459-CDKN1A

    Plasmid

    #217457

    Purpose

    For CDKN1A (p21) knockout

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for CDKN1A
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCR-Zeo

    Plasmid

    #158709

    Purpose

    For cloning and constitutive expression of crRNAs in mycobacterium

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    crRNA
    

    Use

    CRISPR and Synthetic Biology; Mycobacteria expres…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Hsp60

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX459-sgAAVS1

    Plasmid

    #184403

    Purpose

    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    AAVS1-specific sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. U6_crRNA_mCherry

    Plasmid

    #197609

    Purpose

    Expresses a Cas7-11 crRNA and mCherry

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    U6_crRNA_mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pU6-Lb-crRNA

    Plasmid

    #78957

    Purpose

    Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYZ463

    Plasmid

    #187971

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GESTALT_pX330-v1

    Plasmid

    #103061

    Purpose

    plasmid px330 with guide targeting GESTALT v1 through V5 constructs

    Depositor

    Jay Shendure

    Article

    McKenna et al Science. 2016 May 26. pii: aaf7907.

    Insert

    integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJB07

    Plasmid

    #190481

    Purpose

    Targeting plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Upstream & Downstream pyrE deletion region
    gRNA
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX330A_dCas9-1x7

    Plasmid

    #63601

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LRT2B-GO2

    Plasmid

    #136909

    Purpose

    Lentivirus expressing GO2 sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgGO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    hU6

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYZ462

    Plasmid

    #187970

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. JME4599

    Plasmid

    #129660

    Purpose

    NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMZ374

    Plasmid

    #59896

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge