We narrowed to 6,962 results for: tet on

1. pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

   Plasmid

   #199355

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-SUMOstar

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82702

   Purpose

   AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO Empty gRNA Expression Cassette
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry

   Plasmid

   #179431

   Purpose

   Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-coreHS4-mch (SC)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF alpha, pRSV

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199365

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

   Plasmid

   #89874

   Purpose

   expresses DRD2-iTango component in mammalian cells for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   DRD2-V2tail-TevN-BLITz1-TetR-VP16
   

   Use

   AAV

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   short isoform of DRD2 (deletion of 242-270)

   Promoter

   Human synapsin

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo

   Plasmid

   #163084

   Purpose

   rTetR DNA binding domain fused to GBP (anti-GFP nanobody)

   Depositor

   Lacra Bintu

   Article

   Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

   Insert

   anti-GFP nanobody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

   Plasmid

   #179435

   Purpose

   Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-coreHS4-mch (SC)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF alpha, pRSV

   Available since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199368

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOstar-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

   Plasmid

   #179428

   Purpose

   Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-(nospacer)-mch
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF alpha, pRSV

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179430

    Purpose

    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-HS4-mch (SH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

    Plasmid

    #223174

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    RfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

    Plasmid

    #229826

    Purpose

    Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    mScarlet-I
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-Xbp1(S255A)

    Plasmid

    #231595

    Purpose

    Express high ribosome loading circular RNA translation reporter 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Kozak sequence-Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-2xXbp1(S255A)

    Plasmid

    #231596

    Purpose

    Express high ribosome loading circular RNA translation reporter 2xALFATag-10xSunTag-2xXbp1(S255A) pause site under H1 promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Kozak sequence-Tornado-2xALFATag-10xSunTag-2xXbp1(S255A) pause sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179434

    Purpose

    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-HS4-mch (SH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

    Plasmid

    #205540

    Purpose

    Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    TRE3GS

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

    Plasmid

    #221234

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA M6-UGI-T2A-mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179436

    Purpose

    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    HS4-TRE-cit-HS4-mch (DH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199367

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOEu-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199369

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOstar-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid

    #179437

    Purpose

    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    coreHS4-TRE-cit-coreHS4-mch (DC)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJT329 Lenti pEF-rTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE

    Plasmid

    #187339

    Purpose

    rTetR effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    rTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMH005_phiC31_neo-core-5xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid

    #179433

    Purpose

    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    coreHS4-TRE-cit-coreHS4-mch (DC)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMH004_phiC31_neo-HS4-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179432

    Purpose

    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    HS4-TRE-cit-HS4-mch (DH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSL008_phiC31_neo-5xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179426

    Purpose

    Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-5kb-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMH010_phiC31_neo-5xTetO-pEF-H2B-mCitrine-1.2kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179427

    Purpose

    Dual fluorescent reporter construct with 1.2kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-1.2kb-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid

    #119945

    Purpose

    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

    Plasmid

    #161926

    Purpose

    Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    rTetR(SE-G72P)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

    Plasmid

    #164615

    Purpose

    Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.

    Depositor

    Jeffrey Chao

    Article

    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xMS2v5 (3'UTR) and SunTag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

    Plasmid

    #177265

    Purpose

    STREAMING-tag cassette

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag cassette
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

    Plasmid

    #105000

    Purpose

    Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJT218 lenti pEF-rTetR(SE-G72P)-3XFLAG-VP64-T2A-mCherry-BSD-WPRE

    Plasmid

    #188758

    Purpose

    Lentiviral backbone containing VP64 as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    VP64
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid

    #113702

    Purpose

    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179429

    Purpose

    Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-5kb-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA

    Plasmid

    #104984

    Purpose

    Expresses ST-Renilla reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMV10 lenti pEF-rTetR(SE-G72P)-3XFLAG-WWP2(WW)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188756

    Purpose

    Lentiviral backbone containing WWP2 WW domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    WWP2 WW (WWP2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJT228 lenti pEF-rTetR(SE-G72P)-3XFLAG-KIBRA WW-T2A-mCherry-BSD-WPRE

    Plasmid

    #188762

    Purpose

    Lentiviral backbone containing KIBRA WW as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    KIBRA WW (WWC1 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMV5 lenti pEF-rTetR(SE-G72P)-3XFLAG-APBB1(WW)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188751

    Purpose

    Lentiviral backbone containing APBB1 WW domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    APBB1 WW (APBB1 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid

    #113701

    Purpose

    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid

    #119946

    Purpose

    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #161928

    Purpose

    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    Surface marker and Citrine activation reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #161927

    Purpose

    Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    Surface marker and Citrine repression reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188747

    Purpose

    Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

    Plasmid

    #104097

    Purpose

    Expresses TREAT-PK co-localization control in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10 KRAB-T2A-mCherry-BSD-WPRE

    Plasmid

    #188757

    Purpose

    Lentiviral backbone containing ZNF10 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    ZNF10 KRAB (ZNF10 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pJT230 lenti pEF-rTetR(SE-G72P)-3XFLAG-NCOA3-TAD-T2A-mCherry-BSD-WPRE

    Plasmid

    #188763

    Purpose

    Lentiviral backbone containing NCOA3 as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    NCOA3 (NCOA3 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMV9 lenti pEF-rTetR(SE-G72P)-3XFLAG-NOTC2(Notch)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188755

    Purpose

    Lentiviral backbone containing NOTC2 Notch domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    NOTC2 Notch (NOTCH2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104096

    Purpose

    Expresses TREAT-ARE reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Use

    Tags

    12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #196545

    Purpose

    Donor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    DelRosso et al Nature. 2023 Apr;616(7956):365-372. doi:

    Insert

    Surface marker and Citrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. XZ390 (IVT) CMV-TO-T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3

    Plasmid

    #233425

    Purpose

    IVT template for LIDAR reporter mRNA; mCherry as marker, rtetR-NZF as output

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge