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Showing: 1901 - 1950 of 2321 results

  2. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  7. pLGB36

    Plasmid
    #135621
    Purpose
    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection
    Depositor
    Laurie Comstock
    Article
    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-
    Insert
    bfe3
    Use
    Bacteroides suicide vector with inducible counter…
    Tags
    signal sequence for periplasmic targeting
    Expression
    Mutation
    Promoter
    Bacteroides promoter regualted by TetR transcript…
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    135621-plasmid-map-sequence-id-267063

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  8. pMDC32B-AtMIR390a-B/c

    Plasmid
    #51776
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtMIR390a-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51776-plasmid-map-sequence-id-80341

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  10. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid
    #86843
    Purpose
    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.
    Depositor
    Thomas Cech
    Article
    Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d
    Insert
    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    Use
    Tags
    FLAG-HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous TERT promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    86843-plasmid-map-sequence-id-174395

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  12. CRISPseq-BFP-backbone

    Plasmid
    #85707
    Purpose
    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.
    Depositor
    Ido Amit
    Article
    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85707-plasmid-map-sequence-id-170482

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  16. lentiMutB3GALT5catdead-blast

    Plasmid
    #220869
    Purpose
    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    encodes D156A and D243A mutations; also, nucleoti…
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220869-plasmid-map-sequence-id-440865

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  20. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid
    #213965
    Purpose
    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o
    Depositor
    Wensheng Wei
    Article
    Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213965-plasmid-map-sequence-id-422887

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  21. pMDC123SB-AtMIR390a-B/c

    Plasmid
    #51775
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtMIR390a-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51775-plasmid-map-sequence-id-176705

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  23. pCCM

    Plasmid
    #162708
    Purpose
    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162708-plasmid-map-sequence-id-324195

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  25. pMOD_B2103b

    Plasmid
    #91062
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CmYLCV
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91062-plasmid-map-sequence-id-181865

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  26. pFK210B-AtMIR390a-B/c

    Plasmid
    #51777
    Purpose
    Plant expression vector (35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtMIR390a-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51777-plasmid-map-sequence-id-79410

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  27. CRISPseq-mCherry-backbone

    Plasmid
    #85708
    Purpose
    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.
    Depositor
    Ido Amit
    Article
    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85708-plasmid-map-sequence-id-170481

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  28. pMDC32B-AtTAS1c-B/c

    Plasmid
    #51773
    Purpose
    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51773-plasmid-map-sequence-id-80112

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  30. pMDC123SB-AtTAS1c-B/c

    Plasmid
    #51772
    Purpose
    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51772-plasmid-map-sequence-id-80110

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  36. pENTR-AtTAS1c-B/c

    Plasmid
    #51774
    Purpose
    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Gateway-compatible entry clone
    Tags
    Expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51774-plasmid-map-sequence-id-79409

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  38. DHFR-GFP-th-NL1

    Plasmid
    #173007
    Purpose
    For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-GFP-NL1
    Use
    Tags
    DHFR and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173007-plasmid-map-sequence-id-339111

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  40. pCX-SponGee-Kras

    Plasmid
    #134777
    Purpose
    mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Article
    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e
    Insert
    SponGee-Kras
    Use
    Tags
    FLAG and mRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    134777-plasmid-map-sequence-id-266270

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  41. DHFR-GFP-th-GluA1

    Plasmid
    #173008
    Purpose
    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-GFP-th-GluA1
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    173008-plasmid-map-sequence-id-339527

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  42. pCXLE-dCas9VPPH-T2A-GFP-shP53

    Plasmid
    #102897
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    dCas9VPPH-T2A-GFP-shP53
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102897-plasmid-map-sequence-id-194735

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  43. pH7WG2B-OsMIR390-B/c

    Plasmid
    #61465
    Purpose
    Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.
    Depositor
    James Carrington
    Article
    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1
    Insert
    OsMIR390-B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    O. sativa MIR390 precursor sequence including a c…
    Promoter
    OsUBI
    Available since
    March 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    61465-plasmid-map-sequence-id-100075

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  45. pDest27-mBIN1

    Plasmid
    #61086
    Purpose
    Express N-terminal GST tagged-mouse BIN1 in mammalian cells
    Depositor
    Robin Shaw
    Article
    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.
    Insert
    smallest constitutive BIN1excluding exon 7, 11, 13-17
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  46. pDest27-mBIN1+13

    Plasmid
    #61087
    Purpose
    Express N-terminal GST tagged-mouse BIN1+13 in mammalian cells
    Depositor
    Robin Shaw
    Article
    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.
    Insert
    mouse BIN1 with exon 13, excluding exon 7, 11, 14-17
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  47. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    MevT and MBIS operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100167-plasmid-map-sequence-id-193500

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  49. pDest27-mBIN1+17

    Plasmid
    #61096
    Purpose
    Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells
    Depositor
    Robin Shaw
    Article
    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.
    Insert
    mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1901 - 1950 of 2321 results