167,150 results
-
Plasmid#162991PurposeBEAR target plasmid with split mScarlet and disrupted 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK3b(Glu)
Plasmid#50449Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
TagsFlagExpressionMammalianMutationchanged Serine 218 to Glutamic Acid, changed Thre…PromoterCMVAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_1
Plasmid#78203PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (G26C)
ExpressionBacterialMutationG26C (please see depositor comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-3xFLAG-NLS-GFP
Plasmid#198238PurposeLentivector with tet-inducible 3xFLAG-NLS-GFPDepositorInsert3xFLAG-NLS-GFP
UseLentiviralTags3xFLAGExpressionMammalianPromoterTREAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-pHuji
Plasmid#61556PurposeRed fluorescent protein based pH indicator in pDisplay vectorDepositorInsertpHuji
ExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX459_gRNA-AAVS1_hspCas9
Plasmid#193309PurposeCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
UseCRISPRExpressionMammalianMutationnot applicableAvailable SinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shNS
Plasmid#127696PurposeDoxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouseDepositorInsertshNS
UseLentiviralExpressionMammalianAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_synNotch_Gal4VP64
Plasmid#79125PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_Gal4VP64
UseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-23Q
Plasmid#84349PurposeExpression of the human Huntingtin Exon1 protein containing 23Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
LI Intron c1
Plasmid#54433DepositorInsertIntron element for construction of silencing vectors (intron1 of AtWRKY33)
UseCloning vectorAvailable SinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX MLKL(wt)--2A-mCherry-puro
Plasmid#231974PurposeTet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expressionDepositorAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBP2MF
Plasmid#31279DepositorAvailable SinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AMPKAR
Plasmid#192450PurposeExRai-AMPKAR targeted to the nucleus.DepositorInsertExRai-AMPKAR-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoxCDGC-KMet-EGFP
Plasmid#140272PurposeL7Ae-dependent translational repressionDepositorInsertBoxCD
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
GPR50-Tango
Plasmid#66361PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti-mTagBFP2
Plasmid#216126PurposeLentiviral vector for expressing mTagBFP2.DepositorInsertEF1a-mTagBFP2-WPRE
UseLentiviralExpressionMammalianAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221E
Plasmid#108108PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PTEN_p.G129E
Plasmid#81689PurposeGateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEmax-mRNA
Plasmid#204472Purposeplasmid for PEmax mRNA IVTDepositorInsertPrime editor
UseCRISPRTagsnoExpressionMammalianPromoterT7Available SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV-MRLC1-mRuby2-IRES-PuroR
Plasmid#103031PurposeLentiviral expression of mRuby2-tagged MRLC with puromycin selectionDepositorAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFH103
Plasmid#128208PurposecrRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)DepositorInsertcrRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sox2-17
Plasmid#193353PurposeRetroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox)DepositorInsertSox2-17 (Sox2 Mouse)
UseRetroviralExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…Promoter5'LTRAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-KRAS-G12D
Plasmid#116423PurposeLentiviral expression of KRAS G12DDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
FR_HNF4A2
Plasmid#31100DepositorAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HOXB13_#1
Plasmid#70093PurposeExpression of shRNA to human HOXB13, puromycin selectionDepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTET2-lucCP
Plasmid#198353PurposePart of Renilla reporter system, encodes Firefly luciferaseDepositorInsertFirefly Luciferase
UseLuciferaseExpressionMammalianPromoterpTET2Available SinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO HA-AMPKa2 AS
Plasmid#69827PurposeExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible mannerDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
UseLentiviralTagsHAExpressionMammalianPromoterminimal CMV promoter with tetracycline operatorAvailable SinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4
Plasmid#193345PurposetetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector expressing mouse Oct4 + super-Sox + Klf4)DepositorUseLentiviralExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterTetO mini CMVAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC0569 (parts entry vector)
Plasmid#189966PurposeCloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestionDepositorInsertmNeonGreen
ExpressionMammalianPromoterBBa-J23100Available SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-37Q
Plasmid#84351PurposeExpression of the human Huntingtin Exon1 protein containing 37Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT9750-BEAR-mCherry
Plasmid#162990PurposeBEAR target plasmid with split mCherry and disrupted 5' splice siteDepositorInsertmCherry split with an intron between amino acids 119-120
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDZ148
Plasmid#41950DepositorAvailable SinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPD0056_pIVTRup_dCas9-VP64
Plasmid#242542PurposeIVTDepositorInsertdCas9-VP64
UseCRISPRMutationD10AAvailable SinceNov. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPD0055_pIVTRup_MS2-p65-HSF1
Plasmid#242541PurposeIVTDepositorAvailable SinceNov. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
attB-CT-mCherry-linker-attB-AG::pUC-GW-Kan
Plasmid#244900PurposeRecombinase-mediated integrationDepositorInsertmCherry
UseUnspecifiedAvailable SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)
Plasmid#242660PurposeCBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassetteDepositorInsertpAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
UseAAV and CRISPRTagsBPNLSMutationVRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…PromoterCBhAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
N-term AAV-ABE8e-SpCas9(S55R) (CA21)
Plasmid#242659PurposeCBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructsDepositorInsertpAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSMutationSpCas9(S55R)PromoterCBhAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TBD
Plasmid#236737PurposeProtein overexpression of Thalidomide Binding domain of hCRBNDepositorAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_R-PTEN
Plasmid#227433PurposeExpresses the R-PTEN sensor under a CMV promoterDepositorAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX MLKL(1-180)-2A-mCherry-puro
Plasmid#231976PurposeTet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosisDepositorInsertMLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)
UseLentiviralAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only