161,273 results
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pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE
Plasmid#138148PurposeExpresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInsertCasRx
UseLentiviralTagsNESExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)
Plasmid#225753PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScaf
Plasmid#111401PurposePhagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW63.YY1.FKBP.knock-in.BFP
Plasmid#104371PurposeFor knocking in FKBP F36V P2A BFPDepositorInsertFKBP12(F36V) -P2A-BFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-KIRIN1-GR
Plasmid#107417PurposeBacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GRDepositorInsertKIRIN1-GR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cys-3HB-SPYcatcher
Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB
Plasmid#110820PurposeConventional dCas9 repressor-dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER Short P2A
Plasmid#164108PurposeDetect the short Mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP Mt-ER Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-HLA-E SCT-T2A-GFP
Plasmid#205461Purposelentiviral plasmid for expression of HLA-E SCTDepositorInsertHLA-E SCT (HLA-E Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV mRuby3-Synapsin1a
Plasmid#187896PurposeFor labeling pre-synaptic terminalsDepositorInsertSynapsin1a
UseTagsmRuby3ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pHluorin_M153R-CD63-mScarlet
Plasmid#172118PurposeDual-color fluorescent reporter for CD63-positive exosome secretion and uptakeDepositorInsertCD63 (CD63 Human)
UseLentiviralTagsmScarlet and pHluorin_M153R in a small extracellu…ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myosin-IIA-GFP
Plasmid#38297DepositorInsertMyosin II A (Myh9 Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
mKeima-Red-Mito-7
Plasmid#56018PurposeLocalization: Mitochondria, Excitation: 440, Emission: 620DepositorInsertMito (COX8A Human)
UseTagsmKeima-RedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-Histag-Avidin
Plasmid#214836PurposeThis plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-Histag-Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Nanoluc (Nluc)
Plasmid#232656PurposeNanoluc luciferase expression driven CMV promoterDepositorInsertNanoluc
UseLuciferaseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialMutationPromoterT7lacAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-alphaTubulin_C1
Plasmid#189768PurposeConstruct for labelling microtubules in mammalian cells with mScarlet3-alphatubulinDepositorInsertmScarlet3-alphaTubulin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-KRAB
Plasmid#191314PurposepiggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFPDepositorInsertsKRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-polyA
Plasmid#72602Purposeexpress hCas9 byT7 promoter with polyadenine tailsDepositorInsertCas9-polyadenine
UseTags81-bp polyadenylationExpressionMutationPromoterT7Available sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-GFPnanobody
Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
eSrtA(2A-9) pET29b
Plasmid#75145Purposereprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificityDepositorInsertreprogrammed sortase A variant
UseTags6x HisExpressionBacterialMutationS102C A104H E105D K138P K152I N160K K162H T164N K…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
PG13-mCherry
Plasmid#195291PurposemCherry reporter containing 13 copies of the p53-binding consensus sequenceDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR
Plasmid#213481PurposeAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsGFP-KASH
U6-gRNA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 3xHA-KillerRed-OMP25
Plasmid#174544Purposelocalizes KillerRed to mitochondriaDepositorInsert3xHA-KillerRed-OMP25
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSK021 CMV-TO-p53R248Q (FLP-IN)
Plasmid#191167PurposeExpression of human p53 mutant R248Q in mammalian cellsDepositorInsertp53(R248Q) (TP53 Human)
UseTagsExpressionMammalianMutationR248QPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Non tagged Avidin
Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_DA2m
Plasmid#140553PurposeExpresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neuronsDepositorHas ServiceAAV9InsertThe 2nd generation DA sensor GRAB_DA2m (medium affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TET1-dCas9
Plasmid#235593PurposeTet1CD fused to N-terminus of dCas9DepositorInsertTET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)
UseCRISPRTags3xNLS, TET1 CD, and tagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PTK2
Plasmid#23902DepositorInsertPTK2 (PTK2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
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ABE8.20-SpRY
Plasmid#226859PurposeExpresses ABE8.20-SpRY base editor in mammalian cells with eGFP markerDepositorInsertABE8.20-SpRY
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
p58sheLL
Plasmid#37324DepositorInsertHPV58L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-Akt1
Plasmid#53583Purposelentiviral expression of constitutively active human Akt1 and EGFPDepositorInsertcAkt1 (AKT1 Human)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceOct. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-NFAT-eGFP
Plasmid#118031PurposeFluorescent reporter for NFAT activationDepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterminimal promoter with NFAT binding siteAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH424-ssr
Plasmid#198026PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Sm resistance cassette
UseTagsExpressionBacterialMutationPromoterSmR promoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro IRES-EGFP
Plasmid#14430DepositorHas ServiceCloning Grade DNAInsertIRES-EGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG
Plasmid#225241PurposeMammalian expression of iPASnFR-H in the cytosolDepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only
