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Showing: 1721 - 1760 of 167150 results
  1. pYLCRISPR/Cas9P35S-B

    Plasmid
    #66190
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection
    Depositor
    Insert
    Cas9
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. TFORF3549

    Plasmid
    #145025
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.
    Depositor
    Insert
    GFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pNPTS138 Cm

    Plasmid
    #41891
    Depositor
    Type
    Empty backbone
    Use
    Bacterial allelic exchange vector with sacb
    Expression
    Bacterial
    Available Since
    Jan. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
  4. pEGFP-C1-SopF

    Plasmid
    #137734
    Purpose
    Expresses EGFP fused to SopF
    Depositor
    Insert
    SopF
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. lenti-sgRNA puro

    Plasmid
    #104990
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pGEX6P1-DEST

    Plasmid
    #119749
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.
    Type
    Empty backbone
    Tags
    GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. tdTomato-Lysosomes-20

    Plasmid
    #58109
    Purpose
    Localization: Lysosome Membrane, Excitation: 554, Emission: 581
    Depositor
    Insert
    LAMP1
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pPB-C6F/TdTomato

    Plasmid
    #140826
    Purpose
    OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)
    Depositor
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    C-MYC (MYC Human)
    KLF2 (KLF2 Human)
    NANOG (NANOG Human)
    tdTomato
    Expression
    Mammalian
    Mutation
    L104P- please see depositor comments
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3.1-OGT_opt

    Plasmid
    #190858
    Purpose
    For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.
    Depositor
    Insert
    O-GlcNAc Transferase (OGT Human)
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells.
    Promoter
    CMV
    Available Since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1721 - 1760 of 167150 results