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8,883 results

Showing: 1721 - 1760 of 8883 results

  6. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid
    #160734
    Purpose
    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160734-plasmid-map-sequence-id-318595

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  9. pLenti.CAG.H2B-Dendra2.W

    Plasmid
    #51005
    Purpose
    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-Dendra2
    Use
    Lentiviral
    Tags
    histone2B
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51005-plasmid-map-sequence-id-490664

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  10. pET-scFv-T

    Plasmid
    #67843
    Purpose
    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.
    Depositor
    Joanna Bereta
    Article
    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24
    Insert
    antibody 1D8 light kappa chain
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67843-plasmid-map-sequence-id-117725

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  18. pMSCV-IRES-mCherry FP

    Plasmid
    #52114
    Purpose
    a bicistronic IRES-mCherry containing retroviral vector
    Depositor
    Dario Vignali
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Available Since
    May 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52114-plasmid-map-sequence-id-354437

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  35. HaloTag-A2A-adenosine receptor

    Plasmid
    #66995
    Purpose
    A2A-adenosine receptor tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-A2A-adenosine receptor (ADORA2A Human)
    Tags
    Human Growth hormone Signal Sequence- HaloTag
    Expression
    Mammalian
    Mutation
    The signal sequence from human growth hormone was…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66995-plasmid-map-sequence-id-115422

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  37. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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Showing: 1721 - 1760 of 8883 results