4,133 results

1. pBabe B-Raf V600E

   Plasmid

   #17544

   Depositor

   Channing Der

   Article

   Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

   Insert

   B-Raf (BRAF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4xCSL-luciferase

   Plasmid

   #41726

   Depositor

   Raphael Kopan

   Article

   Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

   Insert

   4xCSL binding sites
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains a multimerized high affinity CSL binding…

   Promoter

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. murine TCR OTII-2A.pMIG II

   Plasmid

   #52112

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   P2A (from porcine teschovirus-1)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-HA-pcDNA3.1-

   Plasmid

   #52535

   Purpose

   enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1036 pcDNA3 Myr HA Akt1

   Plasmid

   #9008

   Purpose

   Mammalian expression of human Akt1 with myr, HA tags

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   HA and myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3/er-(n2)oxStayGold(c4)v2.0

   Plasmid

   #186296

   Purpose

   A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   er-(n2)oxStayGold(c4)v2.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sfGFP-C1

   Plasmid

   #54579

   Purpose

   Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

   Depositor

   Geoffrey Waldo, Michael Davidson

   Article

   Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBPZpGAL4DBDUw

   Plasmid

   #26233

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   Zip-GAL4DBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Tet1

    Plasmid

    #60938

    Purpose

    Expresses Tet1 in mammalian cells

    Depositor

    Yi Zhang

    Article

    Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLNCX2 ER:ras

    Plasmid

    #67844

    Purpose

    4-hydroxytamoxifen inducible ER:ras fusion protein

    Depositor

    Masashi Narita

    Article

    Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

    Insert

    ER1a H-RasG12V fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G525R renders the HBD largely insensitive to 17ß‐…

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EBFP2-nuc

    Plasmid

    #36085

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

    Plasmid

    #37120

    Purpose

    DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    TdTomato-EGFP
    

    Use

    AAV and Cre/Lox; Cre-switch

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSIN-PAmCherry-KFERQ-NE

    Plasmid

    #102365

    Purpose

    Lentiviral overexpression of PA-mCherry-KFERQ fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    KFERQ peptide
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid

    #26976

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV5, AAV8, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #64324

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1136- Desmoplakin-GFP

    Plasmid

    #32227

    Depositor

    Kathleen Green

    Article

    Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

    Insert

    Desmoplakin (DSP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDest-mCherry-N1

    Plasmid

    #31907

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-DsRed_IRES_EGFP

    Plasmid

    #92194

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA24N-p50-ligase

    Plasmid

    #87742

    Purpose

    IPTG-inducible expression of p50-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and human p50/NF-kB (amino acids 40-366)

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2FLAG

    Plasmid

    #16331

    Depositor

    Peter Klein

    Insert

    Flag tag
    

    Use

    Vertebrate expression

    Tags

    Flag

    Expression

    Mutation

    Flag tag inserted into pCS2+ polylinker

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.0 FLAG TRPV4

    Plasmid

    #45751

    Depositor

    Robert Lefkowitz

    Article

    Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

    Insert

    TRPV4 (Trpv4 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-28b-RfxCas13d-His

    Plasmid

    #141322

    Purpose

    Plasmid for bacterial expression and purification of RfxCas13d protein

    Depositor

    Ariel Bazzini, Miguel Angel Moreno-Mateos

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

    Plasmid

    #44362

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV; Adeno associated viral vector

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid

    #128604

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-EGFP-C4-Nrf2

    Plasmid

    #21549

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-Tol2

    Plasmid

    #31823

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Insert

    Tol2 Transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJ3H PTP-1B

    Plasmid

    #8601

    Depositor

    Ben Neel

    Article

    Venable et al J Biol Chem. 2000 Jun 16. 275(24):18318-

    Insert

    PTP-1B (PTPN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K103R

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-TORCAR

    Plasmid

    #64927

    Purpose

    FRET-based biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR (EIF4EBP1 Human, Synthetic)
    

    Use

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-HA-Sumo1

    Plasmid

    #21154

    Purpose

    Mammalian expression of human Sumo1 with HA tag

    Depositor

    Junying Yuan

    Article

    Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

    Insert

    pcDNA3-HA-Sumo1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCS2-nCas9n

    Plasmid

    #47929

    Purpose

    expression of an optimized Cas9 for genome-editing in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    nls-zcas9-nls
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HypoxCR

    Plasmid

    #59946

    Purpose

    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

    Depositor

    Anne Le

    Article

    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

    Inserts

    GFP-Pest
    mCherry-Geminin
    

    Use

    Lentiviral

    Tags

    FLAG and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and min CMV

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCI HA NEDD4L

    Plasmid

    #27000

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    NEDD4L (NEDD4L Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Snap-Omp25

    Plasmid

    #69599

    Purpose

    Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    Snap-Omp25
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pdCas9-DNMT3A-EGFP

    Plasmid

    #71666

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti6.2-3xFLAG-V5-ccdB

    Plasmid

    #87072

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-hAR

    Plasmid

    #89078

    Purpose

    mammalian expression of human androgen receptor in pCMV5

    Depositor

    Elizabeth Wilson

    Article

    Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

    Insert

    human androgen receptor (hAR) full-length (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge