163,606 results

1. pDONR221_SLC8A1

   Plasmid

   #132162

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC8A1 (SLC8A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. mCherry-Moesin-N-14

   Plasmid

   #55104

   Purpose

   Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Moesin (MSN Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pMC-1-ZmPLTP-6

   Plasmid

   #197713

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   ZmPLTP promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPGK-T7/2-CD44v9 (fl)

   Plasmid

   #137818

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v9 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length and mutation H48L

   Promoter

   PGK

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pF3BGX-T2A-lexAp65

   Plasmid

   #138393

   Purpose

   RMCE vector for generating C-terminal T2A-lexA-p65 fusion

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   T2A-lexAp65
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_mcherry

   Plasmid

   #167862

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. betaTrCP mVenus reporter

   Plasmid

   #192458

   Purpose

   Fluorescent reporter for beta-TrCP activity in human cells

   Depositor

   Steven Cappell

   Article

   Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

   Insert

   CDC25B (CDC25B Human)
   

   Use

   Lentiviral

   Tags

   SV40 NLS and mVenus

   Expression

   Mammalian

   Mutation

   aa198-338

   Promoter

   EF1alpha

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. betaTrCP mVenus mutant reporter

   Plasmid

   #192459

   Purpose

   Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

   Depositor

   Steven Cappell

   Article

   Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

   Insert

   CdC25B
   

   Use

   Lentiviral

   Tags

   SV40 NLS and mVenus

   Expression

   Mammalian

   Mutation

   aa198-338 of CDC25B. betaTrCP degron sequence (aa…

   Promoter

   EF1alpha

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6PBG

   Plasmid

   #162263

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   COPA (COPA Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pNP1 eforRed trigger

    Plasmid

    #107357

    Purpose

    Trigger sequence for pNP1 eforRed toehold switch

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold trigger 1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBACgus_Sumostar/LRRK2 1867-2186

    Plasmid

    #40386

    Depositor

    Michael J Fox Foundation MJFF

    Inserts

    SUMO
    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    6xHis and HisTEV

    Expression

    Mutation

    WT aa1867-2186

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. mCherry-Ezrin-C-14

    Plasmid

    #55042

    Purpose

    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    NM_003379.4 (EZR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

13. Bet1-mCherry

    Plasmid

    #209848

    Purpose

    Expresses Bet1-mCherry in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Bet1 (BET1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-TMCC1

    Plasmid

    #120932

    Purpose

    Expresses human TMCC1 in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

    Insert

    Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAB064d

    Plasmid

    #79197

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Plac62 luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac62; Ppro1

    Available Since

    Dec. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPGK-T7/2-CD44s (-cyt)

    Plasmid

    #137815

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44s (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSMOX-mNG::LacI-V3

    Plasmid

    #210224

    Purpose

    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    mNeongreen Lac repressor fusion protein
    

    Use

    Trypanosoma brucei expression

    Tags

    Expression

    Mutation

    Promoter

    Pol II readthrough transcription after integration

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SB52

    Plasmid

    #21487

    Depositor

    Shoumo Bhattacharya

    Article

    Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 23, 2009

    Availability

    Academic Institutions and Nonprofits only

19. nLuc_AP4_TEVs_P3mS

    Plasmid

    #119299

    Purpose

    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    

    Use

    Luciferase

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEMS1375

    Plasmid

    #29176

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple88 (GFAP Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pB-6HIS-TEV-mEGFP,IRES-NGFR

    Plasmid

    #164521

    Purpose

    All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AP4mS_PPVs_P3_cLuc

    Plasmid

    #119300

    Purpose

    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJAT13araE

    Plasmid

    #18987

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

    Insert

    araE (araE E.coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pAcGFP1-N1-Dsup-deltaC

    Plasmid

    #90023

    Purpose

    To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal region

    Promoter

    CMV

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPHR-mCherry-NLS

    Plasmid

    #103819

    Purpose

    Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-mCherry-NLS (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMY-MCS-P2A-PuroR-HA

    Plasmid

    #163352

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGMC00018 (aka pRC0082)

    Plasmid

    #195320

    Purpose

    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUC19 PKC alpha

    Plasmid

    #8429

    Depositor

    Frederic Mushinski

    Insert

    Mouse Protein Kinase C alpha (Prkca Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

29. A*68:01:01

    Plasmid

    #203292

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*68:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCIBN-EYFP-hPMLIII

    Plasmid

    #103808

    Purpose

    BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hPMLIII (CIB1, PML Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge