Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,580 results

Showing: 1841 - 1880 of 163580 results

  1. pDIV643

    Plasmid
    #226182
    Purpose
    Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi
    Insert
    uidA marker flanked with targeting regions to KU70 gene in K.phaffii
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TEF1p
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226182-plasmid-map-sequence-id-449156

    Enlarge

  6. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Article
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available Since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119816-plasmid-map-sequence-id-372950

    Enlarge

  7. pAAV-CAG-tdTomato (codon diversified)

    Plasmid
    #59462
    Purpose
    AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter
    Depositor
    Edward Boyden
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    codon diversified
    Promoter
    CAG
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    59462-plasmid-map-sequence-id-423489

    Enlarge

  21. pSNAP-tag (m) Vector

    Plasmid
    #101135
    Purpose
    Expression of protein of interest with SNAP-Tag in Mammalian cells
    Depositor
    Ana Egana, New England Biolabs
    Type
    Empty backbone
    Use
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101135-plasmid-map-sequence-id-194101

    Enlarge

  33. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

    Enlarge

  34. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

    Enlarge

  35. TFORF0801

    Plasmid
    #141707
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ATF3 (ATF3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141707-plasmid-map-sequence-id-391487

    Enlarge

  38. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid
    #212020
    Purpose
    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    UtrCH
    Use
    Tags
    mStayGold(c4)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212020-plasmid-map-sequence-id-419202

    Enlarge

Showing: 1841 - 1880 of 163580 results