165,886 results

1. rAAV2-retro helper

   Plasmid

   #81070

   Purpose

   retrograde AAV helper vector, carrying rep and modified capsid gene

   Depositor

   Alla Karpova, David Schaffer

   Article

   Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

   Inserts

   rep
   rAAV2-retro cap
   

   Use

   AAV and Unspecified; Helper vector

   Mutation

   Pro2Ala, possibly Asp613Gly and changes made to A…

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDZ2087

   Plasmid

   #92414

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

   Insert

   TEV protease
   

   Tags

   MBP (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   tac

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-link-yoEGFP-CaURA3

   Plasmid

   #44872

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEGFP
   

   Expression

   Yeast

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMH-SFB

   Plasmid

   #99391

   Purpose

   Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Available Since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD120

   Plasmid

   #119879

   Purpose

   Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pConstitutive_Che9c60 and Che9c61
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TETv4

   Plasmid

   #167983

   Purpose

   Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   TETv4 (TET1CD-XTEN80-dCas9)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   3xNLS, TET1 catalytic domain, and tagBFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3XFlag-pA-Tn5-Fl

   Plasmid

   #124601

   Purpose

   Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

   Depositor

   Steven Henikoff

   Article

   Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

   Insert

   Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
   

   Tags

   Protein A and Tn5 transposase fusion protein with…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MBP-FUS_FL_WT

   Plasmid

   #98651

   Purpose

   Expresses MBP-tagged full length FUS

   Depositor

   Nicolas Fawzi

   Article

   Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

   Insert

   FUS (FUS Human)
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW empty vector

   Plasmid

   #184708

   Purpose

   Lentiviral empty vector for doxycycline-inducible expression

   Depositor

   Alessia Ciarrocchi, Gloria Manzotti

   Article

   Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCXIP-GFP1-10

    Plasmid

    #68715

    Purpose

    Split GFP assay

    Depositor

    Yutaka Hata

    Article

    Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

    Insert

    GFP1-10
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human SERCA2a (pcDNA3.1+)

    Plasmid

    #75187

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    Jonathan Lytton, David MacLennan

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD63-pEGFP C2

    Plasmid

    #62964

    Purpose

    full length human CD63 cloned into pEGFP C2

    Depositor

    Paul Luzio

    Insert

    CD63 molecule (CD63 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CD63 has 1 silent mutation compared to the coding…

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV2/9 REP-AAP-ΔCAP

    Plasmid

    #205991

    Purpose

    Rep and AAP protein expression for AAV CREATE libraries

    Depositor

    Viviana Gradinaru

    Article

    Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

    Insert

    AAV9 Rep and AAP proteins for CREATE libraries
    

    Use

    AAV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MZB209

    Plasmid

    #228492

    Purpose

    BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)

    Depositor

    Richard Baker

    Insert

    BirA (birA )
    

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. HDAC1 Flag

    Plasmid

    #13820

    Purpose

    Mammalian expression of human histone deacetylase 1 with flag tag

    Depositor

    Eric Verdin

    Article

    Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

    Insert

    HDAC1 (HDAC1 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_502

    Plasmid

    #96923

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-PR rep/cap

    Plasmid

    #197565

    Purpose

    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

    Depositor

    Casey Maguire

    Article

    Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

    Inserts

    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    

    Use

    AAV

    Mutation

    contains 7mer insert PRPPSTH between amino acids …

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN1/G3BP1-iRFP

    Plasmid

    #129339

    Purpose

    Stress granule marker

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    iRFP (from Dr. M. Matsuda)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS185 CMV-Cre

    Plasmid

    #11916

    Purpose

    Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter

    Depositor

    Brian Sauer

    Article

    Sauer et al New Biol. 1990 May . 2(5):441-9.

    Insert

    cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL2680

    Plasmid

    #85581

    Purpose

    Replicating base vector for constructing cpf1/CRISPR editing plasmids

    Depositor

    Himadri Pakrasi

    Article

    Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pICH41331::pU6cm-pegR::EURb7

    Plasmid

    #227695

    Purpose

    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6 composite

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSBtet-GP

    Plasmid

    #60495

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHR_Gal4UAS_tBFP_PGK_mCherry

    Plasmid

    #79130

    Purpose

    Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    tBFP
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCMV-PE2-P2A-GFP

    Plasmid

    #132776

    Purpose

    Prime editing in mammalian cells with co-translational GFP expression

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2-P2A-GFP
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCMV-eNme2-C-ABE8e

    Plasmid

    #185667

    Purpose

    Expresses eNme2-C-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-C
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA3-EGFP-C4-Nrf2

    Plasmid

    #21549

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAnc80L65AAP

    Plasmid

    #92307

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

    Depositor

    Luk Vandenberghe

    Article

    Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

    Insert

    Ancestral AAV Capsid Anc80L65AAP
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    Rep2

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid

    #61358

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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31. pDGM6

    Plasmid

    #110660

    Purpose

    Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.

    Depositor

    David Russell

    Article

    Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.

    Insert

    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
    

    Use

    AAV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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32. pBUE411-GGB

    Plasmid

    #198233

    Purpose

    Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411

    Depositor

    Andrea Gallavotti

    Inserts

    GRF4-GIF1 chimera cassette
    pPLTP::BBM cassette
    

    Use

    CRISPR; Plant binary vector

    Expression

    Plant

    Promoter

    Ubiquitin and maize PLTP

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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33. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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34. pcDNA3-Antares2 c-myc

    Plasmid

    #100027

    Purpose

    A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    Antares2
    

    Tags

    c-myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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35. pDEST53-LRRK2-WT

    Plasmid

    #25044

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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36. pPB_TRE3G_scFV-sfGFP-Prdm9CD

    Plasmid

    #235577

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Prdm9 (Prdm9 Mouse)
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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37. L4440

    Plasmid

    #1654

    Depositor

    Andrew Fire

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, T7p, lacZN, OriF1>>, OriF1<<

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMRX-No-HaloTag7-mGFP-LC3-mRFP

    Plasmid

    #184902

    Purpose

    Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Tags

    HaloTag7, monomeric EGFP, and monomeric RFP

    Expression

    Mammalian

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV-KP1

    Plasmid

    #206504

    Purpose

    AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    KP1 capsid
    

    Use

    AAV

    Promoter

    p5

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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40. pTetOne-mCherry-cNLS

    Plasmid

    #138527

    Purpose

    Inducible expression of mCherry with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    mCherry
    

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE3Gs

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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