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pCRISPRyl_XPR2
Plasmid#84609PurposeIntroduces DSB at XPR2 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
AiP12251-pAAV-AiE0453m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2251)
Plasmid#164453PurposeAiE0453m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neuronsInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBetaGlobinAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiP12965 - pAAV-AiE0444h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2965)
Plasmid#191729PurposeAI plasmid ALIAS: AiP12965. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs), enrichment in dorsal striatum over ventral striatumInsertmTFP1
UseAAVTagsExpressionMutationnonePromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_AXP
Plasmid#84608PurposeIntroduces DSB at AXP locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETNb-cALFA
Plasmid#159989PurposeFor the protein expression with ALFA tag at C-terminus in E. coliDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct
Plasmid#175554PurposeTargeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.DepositorInsertNANOG
UseMouse TargetingTagsFKBPV-HA2-P2A-eGFPExpressionMammalianMutationnonePromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbmCherry
Plasmid#184676PurposeNIR-Fb to mCherryDepositorInsertNIR-FbmCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast HA-POLE2
Plasmid#160809PurposeExpress HA-tagged POLE2DepositorInsertHA-POLE2 (POLE2 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF1687
Plasmid#143836PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHNF1B (HNF1B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TBG-Vector
Plasmid#109396PurposeAAV-CRISPR knockout plasmid with Cre recombinase driven by TBG promoter for direct in vivo genome editing in mouse liverDepositorTypeEmpty backboneUseAAV, CRISPR, Mouse Targeting, and Synthetic Biolo…TagsExpressionMammalianMutationPromoterTBGAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry KNL1 wt hardened
Plasmid#45224DepositorInsertmCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)
UseTagsTEV S15ExpressionMammalianMutationPromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD4
Plasmid#195842PurposeExpresses human FZD4 with an N-terminal HiBiTDepositorInsertFZD4 (FZD4 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTK194
Plasmid#59563PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak mKate opal attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_RRMs
Plasmid#157864Purposeexpress His tagged hnRNPA2 RRMsDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHisExpressionBacterialMutation190stopPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
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tubP-FRT>GAL80-6-FRT>
Plasmid#63173Purposeubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassetteDepositorInsert"Flp-Out" GAL80
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-p18G2A-HA
Plasmid#42327DepositorInsertp18 G2A (LAMTOR1 Human)
UseTagsHAExpressionMammalianMutationG2APromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA
Plasmid#192552PurposeExpresses nuclear localized tdTomato under CAG promoterDepositorHas ServiceAAV PHP.eB and AAV9InserttdTomato
UseAAVTagsNLSExpressionMutationdeleted E273 and G274 compared to FPbase ID: PGG5SPromoterCAGAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
rAAV2-retro helper
Plasmid#81070Purposeretrograde AAV helper vector, carrying rep and modified capsid geneDepositorInsertsrep
rAAV2-retro cap
UseAAV and Unspecified; Helper vectorTagsExpressionMutationPro2Ala, possibly Asp613Gly and changes made to A…PromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SARA
Plasmid#67473PurposeMammalian expression of hSara with stop codon (native expression)DepositorInsertSARA (SAR1B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Eef25UTR-TOPmut-renilla
Plasmid#38236DepositorInsertEef2_TOPm_5UTR/Renilla (Eef2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationThe first nucleotides of the 5' UTR were cha…PromoterEndogenous Eef2Available sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB-ATP
Plasmid#193819PurposeCMV overexpression of ChemoB-ATP in mammalian cell linesDepositorInsertChemoB-ATP
UseTagsExpressionMammalianMutationEBFP2:N39Y, A206K; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP KNL1 wt hardened
Plasmid#45223DepositorInsertGFP KNL1 wt hardened (mutated KNL1 full length that is resisted to KNL1 siRNA) (KNL1 Human)
UseTagsTEV S15ExpressionMutationE172GPromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse cyclin D2
Plasmid#86791Purposebacterial expression of mouse cyclin D2DepositorInsertcyclin D2 (Ccnd2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-ALK5-N-13
Plasmid#62751PurposeLocalization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509DepositorInsertALK5 (TGFBR1 Human)
UseTagsmEmeraldExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLQ6332 - ACTB CRISPRa CLIP Donor
Plasmid#210015PurposeCLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locusDepositorInsertdLbCpf1-miniVPR-T2A-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSTAR-mOrange2-sfGFP
Plasmid#115693PurposeGeneration of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent TimerDepositorInsertSA-mOrange2-sfGFP-SD
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 Y42A/M43A
Plasmid#19940DepositorInsertcullin 1 Y42A/M43A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationCUL1 with a SKP1 binding mutantPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMIR-DsRed-IRES-His-Halo-Tev-Keap1
Plasmid#58240Purposeexpresses halo tagged human Keap1 proteinDepositorInsertKelch like ECH associated protein 1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen
Plasmid#205022Purposemini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 siteDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mRFP-mem
Plasmid#55622PurposeThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.DepositorInsertmRFP-Mem
UseTagsThe N-terminal 20 residues of neuromodulin are fu…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoR-ATP
Plasmid#193820PurposeCMV overexpression of ChemR-ATP in mammalian cell linesDepositorInsertChemoR-ATP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIC113
Plasmid#44434DepositorTypeEmpty backboneUseRetroviralTagsEGFP, s peptide, and tevExpressionMammalianMutationPromoterAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMTS_mScarlet-i_N1
Plasmid#85059PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorInsertMTS (COX8A Human)
UseTagsmScarlet-iExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0538
Plasmid#143410PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPAX2 (PAX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-IGHG1
Plasmid#80795PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotypeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPC TRF2 deltaB deltaM
Plasmid#18008DepositorInserthTRF2 (TERF2 Human)
UseRetroviralTagsExpressionMutationDeleted Basic Domain (aa1-44) and Myb Domain (aa4…PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
SGyA
Plasmid#160292PurposeDrives expression of Cas9 and a TdTomato fluorescent markerDepositorInsertsVas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome
UseTagsT2A GFPExpressionInsectMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE075
Plasmid#176832PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert714
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only
