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XRE-pNL1.3[secNLuc]

Plasmid

#182295

Purpose

Secreted Luciferase reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

Xenobiotic Response Element (CYP1A1 Human)

Tags

secNluc

Expression

Mammalian

Promoter

XRE

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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CRE recombinase

Plasmid

#62730

Purpose

Expresses Cre recombinase protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Cre recombinase

Use

Cre/Lox

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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DsRed-rab11 WT

Plasmid

#12679

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

DsRed and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pLVpuro-CMV-N-3xFLAG

Plasmid

#123223

Purpose

N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Clathrin light chain

Plasmid

#70217

Purpose

Expresses GFP11-clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11-Clathrin light chain (CLTA Human)

Tags

GFP11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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SrtA-PDGFR Tm

Plasmid

#121168

Purpose

pMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR Tm

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

SrtA-PDGFR

Use

Retroviral

Tags

Tomato and flag

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 WT

Plasmid

#121446

Purpose

expression of huamn PD-L1 wildtype in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189923

Purpose

AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e V106W

Use

AAV

Mutation

nSaCas9 KKH, TadA ABE8e V106W

Promoter

EFS

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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RKS-Fzd7/8 subtype NGS Wnt

Plasmid

#159628

Purpose

Express Fzd7/8 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1142 (pSPIN, pBBR1 backbone)

Plasmid

#160730

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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Nme2Cas9_AAV

Plasmid

#119924

Purpose

All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Use

AAV

Tags

NLS-3xHA-NLS and NLS-NLS

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTET2-lucCP-3MS2

Plasmid

#198351

Purpose

Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Firefly Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

pTET2

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pNL [NlucP/MycMax-RE/Hygro] Vector

Plasmid

#236805

Purpose

Express MycMax response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

MycMax (MYC Human)

Use

Luciferase

Tags

NanoLuc (R)

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pC0046-EF1a-PspCas13b-NES-HIV

Plasmid

#103862

Purpose

Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

PspCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and HIV NES

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pTET2-RlucCP

Plasmid

#198352

Purpose

Part of Firefly reporter system, encodes Renilla luciferase

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

pTET2

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-GW

Plasmid

#61702

Purpose

Gateway compatible Y2H vector - AD

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV2/rh10

Plasmid

#112866

Purpose

AAV packaging plasmid expressing Rep/Cap genes

Depositor

James M. Wilson

Insert

Rep2/Cap-rh10

Use

AAV

Promoter

Truncated P5

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57-GFP-2A-Cas9

Plasmid

#170805

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

Depositor

Morten Frodin

Article

Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

Plasmid

#216320

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice donor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet3

Plasmid

#189754

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB402-omega

Plasmid

#74797

Purpose

Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

2xCaMV35S enhancer and omega

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

Plasmid

#216321

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Sfp

Plasmid

#159617

Purpose

Expression plasmid for Sfp phosphopantetheinyl transferase

Depositor

Christopher T Walsh, Jun Yin

Article

Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

Insert

Sfp

Tags

6HIS

Expression

Bacterial

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-p300

Plasmid

#191764

Purpose

Expression of EGFP fused to core p300 histone acetyltransferase

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

FRB-EGFP-p300 core (EP300 Human)

Tags

EGFP (N-terminal of p300 core) and FRB (N-termina…

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-H4-23

Plasmid

#56463

Purpose

Localization: Nucleus/Histones, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

H4 (HIST1H4J Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-CAAX

Plasmid

#86056

Purpose

A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Homo sapiens Harvey rat sarcoma (HRAS Human)

Tags

GFP

Expression

Mammalian

Mutation

contains the region from 825-857 of HRAS as a far…

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Plasmid

#207860

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

Plasmid

#188779

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAW62.YY1.FKBP.knock-in.mCherry

Plasmid

#104370

Purpose

For knocking in FKBP F36V P2A mCherry onto YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-mCherry

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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