161,202 results

1. EF1a-Cas9-2XNES-2ERT2

   Plasmid

   #120551

   Purpose

   Express Cas9, 2xNES and 2 tandem ERT2 in mammalian cells

   Depositor

   Yu Wang

   Article

   Zhao et al Mol Ther Nucleic Acids. 2018 Dec 7;13:20

   Insert

   Cas9, NES and ERT2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. YEE-BE4max

   Plasmid

   #138157

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YEE)-nSpCas9-UGI-UGI
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. ch-TOGKDP-GFP

   Plasmid

   #69112

   Purpose

   For mammalian expression of knockdown-proof human ch-TOG tagged with EGFP.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Insert

   Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Silent mutations to confer shRNA resistance.

   Promoter

   CMV

   Available since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT3TS-RfxCas13d-HA

   Plasmid

   #141320

   Purpose

   Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-iGluSnFR4f

   Plasmid

   #234444

   Purpose

   Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f
   

   Use

   Tags

   6xHis-Xpress epi

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP rbFOX1

   Plasmid

   #63085

   Purpose

   Mammalian expression of EGFP tagged FOX1

   Depositor

   Nicolas Charlet-Berguerand

   Insert

   RBFOX1 (RBFOX1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-DRP1 K38A

   Plasmid

   #232350

   Purpose

   Expresses K38A variant of human DRP1 with mCherry tag

   Depositor

   Samantha Lewis

   Article

   Begeman et al bioRxiv [Preprint]. 2024 Aug 17:2024.08.

   Insert

   DRP1 (DNM1L Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   K38A

   Promoter

   CMV

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRPA70-IRES-RPA32-IRES-RPA14

   Plasmid

   #211432

   Purpose

   mammalian expression of human RPA70 and RPA32 and RPA14

   Depositor

   Helmut Pospiech

   Insert

   RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. EJ2GFP-puro

   Plasmid

   #44025

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

   Insert

   EJ2GFP egfp-based chromosomal break reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   insertion of I-SceI, 3 frame stop, and flanking m…

   Promoter

   pCAGGS

   Available since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL2-p21 promoter-Luc

    Plasmid

    #33021

    Depositor

    Martin Walsh

    Article

    Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

    Insert

    p21 promoter (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    p21

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX304 GFP

    Plasmid

    #193687

    Purpose

    Constitutive lentiviral expression of GFP

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRPC1-FLAG

    Plasmid

    #24408

    Depositor

    Craig Montell

    Article

    Xu et al Cell. 1997 Jun 27. 89(7):1155-64.

    Insert

    TRPC1 (TRPC1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGB1_alpha1

    Plasmid

    #68224

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF-CjPglB

    Plasmid

    #199305

    Purpose

    pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    CjPglB with flag tag
    

    Use

    Tags

    Flag tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL003

    Plasmid

    #215335

    Purpose

    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)

    Depositor

    Gene-Wei Li

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pSpank

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCgCas9_recT

    Plasmid

    #153337

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-BCL2

    Plasmid

    #116717

    Purpose

    Lentiviral expression of BCL2

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BCL2 (BCL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRL_U2AF1_WT_mCherry

    Plasmid

    #84017

    Purpose

    expression of U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. TL024_lenti_hSyn:HaloTag-GluA1

    Plasmid

    #192517

    Purpose

    Expression of AMPA GluA1 with N terminal HaloTag fusion

    Depositor

    Adam Cohen

    Article

    Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

    Insert

    HaloTag-GluA1
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. 5aa SunTag VP64 EVD

    Plasmid

    #115483

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pACYCT2

    Plasmid

    #45799

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

    Plasmid

    #216668

    Purpose

    Expresses membrane-bound mCherry in mammalian cells

    Depositor

    Leonardo Morsut

    Insert

    mCherry
    

    Use

    Tags

    PDGFR Transmembrane Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEB1-mVenus NB

    Plasmid

    #103986

    Purpose

    Plasmid encoding mVenus NB

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus NB
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. TFORF1500

    Plasmid

    #143960

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RBL2 (RBL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. secreted BirA-Flag

    Plasmid

    #64395

    Purpose

    Flag-tagged biotinylation enzyme for co-expression with bio-proteins

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    BirA (birA E. coli)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mTagBFP2-Rab5a-7

    Plasmid

    #55322

    Purpose

    Localization: Ras GTPase, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Rab5a
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

27. pX333

    Plasmid

    #64073

    Purpose

    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    3XFLAG-Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CA-NFAT2

    Plasmid

    #11102

    Depositor

    Anjana Rao

    Article

    Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

    Insert

    NFAT2 (Nfatc1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Constitutively active: multiple serine to alanine…

    Promoter

    Available since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-UbC-rtTA-Ngn2:2A:EGFP

    Plasmid

    #127288

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

    Insert

    Ngn2:2A:Ascl1 (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and EGFP linked via 2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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