We narrowed to 23,697 results for: Spr

1. p426-SNR52p-gRNA.csr-1.Y-SUP4t

   Plasmid

   #68060

   Purpose

   gRNA for csr-1 locus in N. crassa

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   gRNA for csr-1 locus
   

   Use

   CRISPR; N. crassa, fungi

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRU53

   Plasmid

   #167679

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. piCRg Entry

   Plasmid

   #58904

   Purpose

   Cas9/gRNA Entry expression plasmid

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   hSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PU6::klp-12_sgRNA

   Plasmid

   #46170

   Purpose

   klp-12 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   klp-12 targeting sgRNA (klp-12 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAC-CR7T-gRNA2.1-nlsBFP

   Plasmid

   #170515

   Purpose

   gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   CR7T

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRU54

   Plasmid

   #167680

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

   Plasmid

   #74709

   Purpose

   Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

   Depositor

   Eugene Yeo

   Article

   Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

   Insert

   U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

8. dCas12j2 KRAB

   Plasmid

   #188508

   Purpose

   Expresses FLAG tagged dCas12j2 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D394A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ141A2.0

   Plasmid

   #99896

   Purpose

   Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR1056

    Plasmid

    #71824

    Purpose

    Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRU293

    Plasmid

    #167688

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pV132-vCas9

    Plasmid

    #203223

    Purpose

    Mammalian expression of vCas9

    Depositor

    Vikash Chauhan, Phil Sharp, Robert Langer

    Article

    Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    vCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    S55R R976A K1003A T1314R

    Promoter

    CBh

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CASI-Cas9C

    Plasmid

    #80932

    Purpose

    Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNW3

    Plasmid

    #53372

    Purpose

    Csy4 and Cas9 D10A nickase expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9n (D10A)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pADH119

    Plasmid

    #90985

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pADH147

    Plasmid

    #90991

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX458_TEAD1_1

    Plasmid

    #86356

    Purpose

    Encodes gRNA for 3' target of human TEAD1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against TEAD1 (TEAD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJG80

    Plasmid

    #89282

    Purpose

    Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

    Depositor

    Daniel Voytas

    Article

    Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pColE1_sgRNA_M1S_AmpR

    Plasmid

    #119153

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pColE1_sgRNA_M1NS_AmpR

    Plasmid

    #119155

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFC331

    Plasmid

    #87843

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    argB
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTnsABC

    Plasmid

    #160031

    Purpose

    Expresses V. cholerae TnsABC

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    no

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-T3-hCasD10A-pA

    Plasmid

    #51638

    Purpose

    Expresses Cas9-nickase in mammalian cells and zygotes.

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

    Insert

    Codon-optimized Cas9 D10A
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

25. sgRNA1_Tet-inducible Luciferase reporter

    Plasmid

    #64161

    Purpose

    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for Tet-inducible Luciferase reporter
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAN-PBAD-sgRNA-A2NT

    Plasmid

    #62257

    Purpose

    expression of A2NT sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A2NT sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRU323

    Plasmid

    #167690

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NAX_CAGGS_CAS9_mcherry

    Plasmid

    #167862

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRU292

    Plasmid

    #167687

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pColE1_sgRNA_M4NS_AmpR

    Plasmid

    #119156

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. NAX_CAGGS_CAS9_m4_Puro

    Plasmid

    #167871

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRU61

    Plasmid

    #167692

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::asCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PB-EEA-g1-PGK-Puro

    Plasmid

    #102908

    Purpose

    PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCK760

    Plasmid

    #192643

    Purpose

    Expresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    BBa_J23110-sfGFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23110

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKEW106-MbCas12a-58069

    Plasmid

    #115653

    Purpose

    Expresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR control

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    MbCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG-Cas12i-Max-T2A-EGFP

    Plasmid

    #188268

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i-Max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-Cas12i-HiFi-T2A-EGFP

    Plasmid

    #188269

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i-HiFi
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKEW336-MBP-TEV-MbCas12a-33362

    Plasmid

    #115670

    Purpose

    Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    MbCas12a
    

    Use

    CRISPR

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFYF1327 EGFP Site#2

    Plasmid

    #47512

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKEW107-MbCas12a-33362

    Plasmid

    #115654

    Purpose

    Expresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR control

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    MbCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. MSP2283

    Plasmid

    #70702

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pYPQ-Cas12j2

    Plasmid

    #173924

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Insert

    NLS-Cas12j2-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pV1539

    Plasmid

    #111434

    Purpose

    Genedrive entry vector for Candida albicans

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. U6-spTRE3G-CMV-mTagBFP2

    Plasmid

    #102854

    Purpose

    A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-TRE3G-sgRNA; mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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45. NAX_CAGGS_CAS9_KanR neo

    Plasmid

    #167861

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pRU52

    Plasmid

    #167678

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. psgRNA2.02-PP7-ccdB

    Plasmid

    #99912

    Purpose

    Basic cloning vector for the expression of PP7 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. pRU51

    Plasmid

    #167677

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. psgRNA2.02-MS2-ccdB

    Plasmid

    #99911

    Purpose

    Basic cloning vector for the expression of MS2 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pYPQ-dpcoCas9-EV2.1

    Plasmid

    #158411

    Purpose

    CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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