4,133 results

1. pcDNA3-S33Y Beta-catenin

   Plasmid

   #19286

   Purpose

   Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S33Y (presumptive GSK3β phosphorylation site)

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEB-C5

   Plasmid

   #28213

   Purpose

   5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

   Depositor

   Linzhao Cheng

   Article

   Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

   Insert

   Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-C1-FLAG-Ku80

   Plasmid

   #46958

   Purpose

   Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku80 (XRCC5 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-Foxo1

   Plasmid

   #17551

   Depositor

   Domenico Accili

   Article

   Frescas et al J Biol Chem. 2005 May 27. 280(21):20589-

   Insert

   pEGFP-N1 Foxo1 (Foxo1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EMTB-mCherry

   Plasmid

   #26742

   Depositor

   William Bement

   Article

   Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

   Insert

   ensconsin (MAP7 Human)
   

   Use

   Probe for microtubules

   Tags

   2x mCherry and Myc

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ERmoxGFP

   Plasmid

   #68072

   Purpose

   mammalian expression of ER localized moxGFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Insert

   moxGFP
   

   Use

   Tags

   KDEL ER retention sequence and bovine prolactin s…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCI-EGFP-NR2b wt

   Plasmid

   #45447

   Depositor

   Andres Barria, Robert Malinow

   Article

   Barria et al Neuron. 2002 Jul 18;35(2):345-53.

   Insert

   NR2B (Grin2b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EGFP inserted after the predicted signal peptide …

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLTS

   Plasmid

   #59386

   Purpose

   Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Type

   Empty backbone

   Use

   Genome engineering

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMV306hsp+LuxG13

   Plasmid

   #26161

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   Bacterial luciferase operon + G13 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK5-HA-Ubiquitin-K11

    Plasmid

    #22901

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K11 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTiger

    Plasmid

    #1728

    Purpose

    This is a 3rd generation lentiviral transfer vector (highest titer) from Nolan lab's FELIX vector system

    Depositor

    Garry Nolan

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EGFP-Tpr

    Plasmid

    #35024

    Depositor

    Larry Gerace

    Article

    Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

    Insert

    Tpr (TPR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 2X PRE TK luc

    Plasmid

    #11350

    Depositor

    Donald McDonnell

    Article

    Giangrande et al Mol Cell Biol. 2000 May . 20(9):3102-15.

    Insert

    PRE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mTagBFP2-Farnesyl-5

    Plasmid

    #55295

    Purpose

    Localization: Plasma Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pENTR1A no ccDB (w48-1)

    Plasmid

    #17398

    Purpose

    Gateway entry 1A vector, no ccDB gene

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG_smFP HA

    Plasmid

    #59759

    Purpose

    encodes multiple copies of an epitope tag HA

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX601-GFP

    Plasmid

    #84040

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with GFP

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV Luciferase PGK-hygro

    Plasmid

    #18782

    Depositor

    Scott Lowe

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Human ICAM-1

    Plasmid

    #8632

    Depositor

    Timothy Springer

    Article

    Staunton et al Cell 1988 Mar 25;52(6):925-33.

    Insert

    ICAM-1 (ICAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-smURFP

    Plasmid

    #80349

    Purpose

    Lentiviral transfer vector under the CMV promoter that expresses smURFP.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Insert

    smURFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CAV1-mCherry

    Plasmid

    #27705

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    monomeric Cherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Flag-HA-USP2

    Plasmid

    #22577

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP2 (USP2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CMV-R-GECO1

    Plasmid

    #32444

    Purpose

    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCXLE-hMLN

    Plasmid

    #27079

    Purpose

    Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    C-MYC, LIN28, NANOG (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. XBP-1 2: pFLAG.XBP1p.CMV2

    Plasmid

    #21833

    Depositor

    David Ron

    Article

    Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

    Insert

    XBP-1 processed/spliced (Xbp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV2.5-THP-GFP

    Plasmid

    #80336

    Purpose

    Fluorescent Reporter for Dopaminergic Neuron Differentiation

    Depositor

    Kwang-Soo Kim

    Article

    Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

    Insert

    rat TH promoter (Th Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Tyrosine hydroxylase

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mEmerald-Integrin-Beta1-N-18

    Plasmid

    #54129

    Purpose

    Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Integrin-Beta1 (ITGB1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. GFP-PIPK1 gamma 90

    Plasmid

    #22299

    Depositor

    Pietro De Camilli

    Article

    Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

    Insert

    human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Myc-PSF-WT

    Plasmid

    #35183

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

    Insert

    PSF (SFPQ Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GLP1R-Tango

    Plasmid

    #66295

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GLP1R (GLP1R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. phage UbiC G3BP1-GFP-GFP

    Plasmid

    #119950

    Purpose

    Lentiviral vector expressing G3BP1-GFP fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-VSVN

    Plasmid

    #64087

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain nucleoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV nucleoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti6-DEST PINK1-V5 WT

    Plasmid

    #13320

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDH-puro-CMV-VC3AI

    Plasmid

    #78907

    Purpose

    Indicator of caspase-3-like protease activity

    Depositor

    Binghui Li

    Article

    Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

    Insert

    VC3AI
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mutation

    S320A, deletion of AA1-200

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MSCV JMJD3

    Plasmid

    #21212

    Depositor

    Paul Khavari

    Article

    Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pgRNA-humanized

    Plasmid

    #44248

    Purpose

    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMXs-3XFLAG-EGFP-OMP25

    Plasmid

    #83354

    Purpose

    For tagging mitochondria with Sigma FLAG epitopes

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XFLAG-EGFP-OMP25
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCX-cMyc

    Plasmid

    #19772

    Purpose

    Non-integrating (episomal) expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Science. 2008 Nov 7. 322(5903):949-53.

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-VSVP

    Plasmid

    #64088

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV phosphoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51087

    Purpose

    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa 1.3 kb

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge