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Showing: 2241 - 2280 of 157643 results

  2. pDule- Mj Acd A9

    Plasmid
    #197651
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Ryan Mehl
    Article
    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197651-plasmid-map-sequence-id-389657

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  5. eIF2a 1

    Plasmid
    #21807
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21807-plasmid-map-sequence-id-351672

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  7. pBEST-p15a-pBAD-UTR1-TetR-T500

    Plasmid
    #45787
    Depositor
    Richard Murray
    Insert
    Tetracycline repressor
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    45787-plasmid-map-sequence-id-64258

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  12. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  13. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234453
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234453-plasmid-map-sequence-id-465748

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  17. pFA6a-link-ymScarletI3-URA3

    Plasmid
    #224075
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Use
    Tags
    ymScarletI3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224075-plasmid-map-sequence-id-460510

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  18. pFA6a-link-ymScarletI3-Kan

    Plasmid
    #224077
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Use
    Tags
    ymScarletI3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224077-plasmid-map-sequence-id-460512

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  23. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

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  25. pBTK1050

    Plasmid
    #209540
    Purpose
    BTK Type 1 homology arm for Golden Gate assembly
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv 2023.09.19.558440
    Insert
    Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209540-plasmid-map-sequence-id-422358

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  26. pBTK1051

    Plasmid
    #209541
    Purpose
    BTK Type 5 homology arm for Golden Gate assembly
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv 2023.09.19.558440
    Insert
    Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 2, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209541-plasmid-map-sequence-id-421604

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  27. pLCS108

    Plasmid
    #209329
    Purpose
    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mCherry fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209329-plasmid-map-sequence-id-420443

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  31. Donor-PIGA

    Plasmid
    #153530
    Purpose
    Used as a donor vector for the targeted correction of a mutation in PIGA exon 6
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    PIGA (a 1,991-bp fragment from intron 5 and exon 6)
    Use
    Donor plasmid
    Tags
    N/A
    Expression
    Mutation
    No mutation
    Promoter
    No promoter
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    153530-plasmid-map-sequence-id-319287

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  36. MSCV-IRES-GFP

    Plasmid
    #20672
    Purpose
    Depositor
    Tannishtha Reya
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20672-plasmid-map-sequence-id-203241

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  38. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid
    #139997
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139997-plasmid-map-sequence-id-352446

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Showing: 2241 - 2280 of 157643 results