163,584 results

1. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216251

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgSEC61B.N1 (SEC61B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQF

   Plasmid

   #48095

   Purpose

   Cumate-inducible expression of 3xFLAG-tagged fusion protein

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MSCV-N 16E5

   Plasmid

   #37874

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   HPV 16 E5 (E5 )
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   PKG

   Available Since

   July 25, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1-Hygro(+)-PSTCD-cGAS

   Plasmid

   #102605

   Purpose

   Mammalian expression of cGAS fused to PSTCD tag

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   cGAS (CGAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1-pA-DddA11_NT

   Plasmid

   #232443

   Purpose

   ProteinA-DddA11_NT for D&Dseq (sc TFs molecular footprint)

   Depositor

   Ivan Raimondi

   Article

   Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

   Insert

   pA-DddA_NT
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

   Plasmid

   #232880

   Purpose

   Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

   Depositor

   Paul DeCaen

   Article

   DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

   Insert

   ADP-ribosylation factor-like protein 13B (ARL13B Human)
   

   Use

   Lentiviral

   Tags

   GFP and RGECO1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-SLCO2B1_STOP

   Plasmid

   #161032

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLCO2B1 (SLCO2B1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pINTO-NFH::hSNRNP70

   Plasmid

   #65926

   Purpose

   Expression of human SNRNP70 fused to Flag-HA tag (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Two silent mutations

   Promoter

   CMV/TO and CMV/TetO2 (T-REx)

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-YTB

   Plasmid

   #26721

   Depositor

   Edward Callaway

   Article

   Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

   Insert

   venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Rabies virus (strain SAD B19), Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRST7-ER beta (long)

    Plasmid

    #11356

    Depositor

    Donald McDonnell

    Article

    Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

    Insert

    ER beta long (ESR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pCAGGS/ES-EnvA-VSVg-WPRE-pA

    Plasmid

    #87221

    Purpose

    to package wild type EnvA psudotyped lentivirus

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    EnvA-VSVg
    

    Use

    Mouse Targeting and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GluA1 (px330:2-guideCas9)

    Plasmid

    #169417

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-Mito-HyPerGRt

    Plasmid

    #173198

    Purpose

    AAV transfer plasmid for a HyPer7 and tdTomato fusion under hSyn promoter

    Depositor

    Huiwang Ai

    Article

    Li et al ACS Sens. 2022 Feb 28. doi: 10.1021/acss

    Insert

    Hyper7-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMiniT:SEP-GluA1 TKIT donor

    Plasmid

    #169418

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA1 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-FRT-EF1a-TVA-2A-N2cG

    Plasmid

    #172361

    Purpose

    Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AiP12857 - pAAV-AiE0674m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2857)

    Plasmid

    #224013

    Purpose

    AiE0674m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSV2 neuNT

    Plasmid

    #10918

    Depositor

    Bob Weinberg

    Article

    Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

    Insert

    neuNT (Erbb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

18. SAP145

    Plasmid

    #11239

    Depositor

    Robin Reed

    Article

    Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

    Insert

    SAP145 (SF3B2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pGEX2T E7

    Plasmid

    #13634

    Depositor

    Karl Munger

    Article

    Wu et al J Virol. 1993 Apr . 67(4):2402-7.

    Insert

    HPV 16 E7 (E7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

20. CYP51A1 (3I3K)

    Plasmid

    #25161

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP51A1_cw_09 (CYP51A1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acid residues 54-502

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

21. p3036 GST-HPV-16 E6

    Plasmid

    #10849

    Depositor

    Peter Howley

    Article

    Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

    Insert

    HPV16 E6 (E6 Human papillomavirus)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

22. mKeima-Red-Mito-7

    Plasmid

    #56018

    Purpose

    Localization: Mitochondria, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mKeima-Red

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MBP-CRY1-PHR

    Plasmid

    #26035

    Depositor

    Johann Deisenhofer

    Article

    Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    CRY1 (CRY1 Mustard Weed)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pBabe puro-eIF4E

    Plasmid

    #33252

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    eIF4E (EIF4E Human)
    

    Use

    Retroviral

    Tags

    untagged

    Expression

    Mammalian

    Mutation

    unmodified

    Promoter

    SV40

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pLX304 CMV OMM-FKBP-V5-sTurboID (N)

    Plasmid

    #153006

    Purpose

    Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    OMM (Tom20)-sTurboID (N) (L73)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. KE-009

    Plasmid

    #133441

    Purpose

    Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

    Depositor

    Gerhard Wagner

    Insert

    EIF4H (EIF4H Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRST7-ER beta (short)

    Plasmid

    #11357

    Depositor

    Donald McDonnell

    Article

    Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

    Insert

    ER beta short (ESR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2006

    Availability

    Academic Institutions and Nonprofits only

28. HC078 (3xHA-Atg13)

    Plasmid

    #59544

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Use

    Tags

    3xHA

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pRK5M-TIMP3

    Plasmid

    #31715

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    TIMP3 (TIMP3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PP7 guide

    Plasmid

    #86168

    Purpose

    Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    PP7 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pErbB2-ECFP

    Plasmid

    #66945

    Purpose

    Mammalian expression of rat ErbB2 tagged with ECFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al J Cell Sci. 2006 Jan 1;119(Pt 1):85-95.

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

32. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CMV::TVA/tRFP

    Plasmid

    #164486

    Purpose

    Dual CMV promoter plasmid to express TVA and TurboRFP

    Depositor

    Hollis Cline

    Article

    Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

    Inserts

    TVA (CD320 Chicken)
    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    sCMV and sCMV IE94

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ClCry4 Y319D

    Plasmid

    #160649

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 PHR Y319D
    

    Use

    Tags

    Hexa his

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. YIp204-PADH1-AFB2

    Plasmid

    #99531

    Purpose

    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA4-ZfCry4

    Plasmid

    #136242

    Purpose

    Protein expression of Zebrafish (Zf) cytochrome 4 with a C terminal FLAG tag

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

    Insert

    ZfCry4
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D196H- Please see depositor comments below

    Promoter

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MSCV-N 18E5

    Plasmid

    #37882

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPV 18 E5 (E5 HPV)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

38. FLT1-KDR

    Plasmid

    #86034

    Purpose

    chimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2

    Depositor

    Christie Thomas

    Article

    Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

    Insert

    human VEGFR1-VEGFR2 hybrid (FLT1, KDR Human)
    

    Use

    Tags

    HA and t-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RabV CVS-N2c(deltaG)-APEX2-Venus

    Plasmid

    #73467

    Purpose

    Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    APEX2-Venus
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pQTEV-PFDN2

    Plasmid

    #31329

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    prefoldin 2 (PFDN2 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge