165,744 results

1. pCK007 pME KalTa4

   Plasmid

   #195965

   Purpose

   Gateway pME Gal4 variant

   Depositor

   Christian Mosimann, Alexa Burger

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   KalTA4 (Gal4)
   

   Use

   Other

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-Dcp1a

   Plasmid

   #153972

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1a

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA decapping enzyme 1a (DCP1A Human)
   

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBtet-BP

   Plasmid

   #60496

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   TCE & RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_nV5-AMOTL1

   Plasmid

   #172998

   Purpose

   constitutive expression of V5-tagged AMOTL1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOTL1 (AMOTL1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

   Plasmid

   #229678

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   monomeric StayGold, HA

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lamp1_mStayGold

   Plasmid

   #221902

   Purpose

   Expresses mStayGold fused to Lamp1

   Depositor

   Janelia Integrative Imaging

   Insert

   Lamp1_mStayGold
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBR1k-GFPuv

   Plasmid

   #106382

   Purpose

   IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-PE2max-BSD

   Plasmid

   #191102

   Purpose

   Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   PEmax-P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-MBP-super TEV protease

   Plasmid

   #171782

   Purpose

   Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   MBP-sTEV
   

   Tags

   Arg6 tag and Internal His6 tag

   Expression

   Bacterial

   Mutation

   No internal TEV cleavage site between the MBP and…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-sgRNA EF1Alpha-puro-T2A-BFP

    Plasmid

    #60955

    Purpose

    Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSP2N2

    Plasmid

    #29520

    Depositor

    Stephen Sligar

    Article

    Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

    Insert

    MSP2N2
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    tandem MSP; His-tag on N-terminus followed by spa…

    Available Since

    May 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

    Plasmid

    #60661

    Purpose

    Flp-dependent expression of Kir2.1-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    FLEX- Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
    

    Use

    AAV

    Promoter

    EF1α

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. P4-PE

    Plasmid

    #211375

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    P4-PE
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRZero

    Plasmid

    #120275

    Purpose

    Blunt-end cloning vector of PCR-products using ZERO-backgound system

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-SFFV-dCas9-BFP-KRAB

    Plasmid

    #46911

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP-KRAB fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, HA, and KRAB domain

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Emerin pEGFP-C1 (637)

    Plasmid

    #61993

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Emerin (EMD Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 MDM2 WT

    Plasmid

    #16233

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR_PGK_antiCD19_synNotch_Gal4VP64

    Plasmid

    #79125

    Purpose

    Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    antiCD19_synNotch_Gal4VP64
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag-HRD1

    Plasmid

    #231688

    Purpose

    Expresses human HRD1 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    HRD1 (SYVN1 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGL3-Sox2

    Plasmid

    #101761

    Purpose

    Sox2 promoter reporter

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    SRY-box 2 promoter (SOX2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLX313-Renilla luciferase

    Plasmid

    #118016

    Purpose

    Constitutive expression of Renilla luciferase

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Renilla luciferase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHR_pGK_LaG17_synNotch_Gal4VP64

    Plasmid

    #79127

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17_synNotch_Gal4DBDVP64
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSEVA331Bb

    Plasmid

    #78269

    Purpose

    pSEVA331 with Biobrick multiple cloning site

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Type

    Empty backbone

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV CEPIA3mt

    Plasmid

    #58219

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA3mt
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. pTNS3

    Plasmid

    #63127

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    P1 and Ptac

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. AiP11633 - pAAV-hsA2-AiE0140h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1633)

    Plasmid

    #163503

    Purpose

    Direct-expressing SYFP2 AAV Virus

    Depositor

    Boaz Levi, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Promoter

    MinRho

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP-NR2A

    Plasmid

    #17924

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    NMDAR2A (Grin2a Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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30. MS2_GFP

    Plasmid

    #61764

    Purpose

    Expresses MS2 coat protein fused to GFP

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    MS2 coat protein fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    Ubc promoter

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ZsGreen1-N1

    Plasmid

    #54702

    Purpose

    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    ZsGreen1

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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33. pScaf-3024.1

    Plasmid

    #111404

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 3024 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-3024
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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34. pLEX_305-C-dTAG

    Plasmid

    #91798

    Purpose

    Lentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG system

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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35. MaLionG

    Plasmid

    #113906

    Purpose

    Green ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionG
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Luciferase-pcDNA3

    Plasmid

    #18964

    Depositor

    William Kaelin

    Article

    Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

    Insert

    Firefly Luciferase
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pArk313

    Plasmid

    #200257

    Purpose

    Ark313 rep/cap

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    Ark313 Rep/Cap
    

    Use

    AAV

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. attP-puro donor

    Plasmid

    #182139

    Purpose

    attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Expression

    Mammalian

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mEGFP

    Plasmid

    #18696

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    monomeric EGFP
    

    Expression

    Mammalian

    Mutation

    A206K mutation

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLKO.1_puro_shNRF2-1

    Plasmid

    #230086

    Purpose

    shRNA silencing human NRF2 gene

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge