8,677 results

1. pBB550

   Plasmid

   #27396

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 . 7():32.

   Insert

   dnaK, dnaJ, GroESL
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-D1R

   Plasmid

   #89160

   Purpose

   Express vaccinia virus D1R in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D1R
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promotor

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 8xNFAT-ZsG-hCD8

   Plasmid

   #153417

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pU6-Sp-pegRNA-RNF2_+5GtoT

   Plasmid

   #135957

   Purpose

   S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   RNF2_5GtoT pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMV306hsp+LuxG13

   Plasmid

   #26161

   Depositor

   Siouxsie Wiles, Brian Robertson

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   Bacterial luciferase operon + G13 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. Klotho (secreted)

   Plasmid

   #17713

   Depositor

   Hal Dietz

   Article

   Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

   Insert

   Klotho (KL Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-M4-AR

   Plasmid

   #171240

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

   Depositor

   Steven Balk

   Article

   Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

   Insert

   3xFlag-tagged human androgen receptor (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNLS-iRFP670

   Plasmid

   #45466

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP670
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

   Promoter

   CMV

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2 Notch1 Full Length-6MT

   Plasmid

   #41728

   Depositor

   Raphael Kopan

   Article

   Schroeter et al Nature. 1998 May 28;393(6683):382-6.

   Insert

   Notch-1 Full Length (Notch1 Mouse)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   L1997V; Contains murine Notch-1 protein up to ami…

   Promoter

   Available since

   Dec. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. plenti-CAG-gate-FLAG-IRES-GFP

    Plasmid

    #107398

    Purpose

    Lentiviral gateway destination vector

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMIR-FLAG-MLL-AF9

    Plasmid

    #71444

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-AF9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flag-HA-OTUB2

    Plasmid

    #22552

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUB2 (OTUB2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSLCAR-CD19-CD3z

    Plasmid

    #135993

    Purpose

    Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-3z-P2A-EGFP (CD247, CD28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-PRB

    Plasmid

    #89130

    Purpose

    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-B (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pICH86966::AtU6p::sgRNA_PDS

    Plasmid

    #46966

    Purpose

    Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    AtU6p::sgRNA_PDS
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-N1-human lyn–GFP

    Plasmid

    #35958

    Purpose

    Expresses human lyn protein codon optimized for zebrafish expression.

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

    Insert

    LYN (LYN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    cmv

    Available since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-FUSN-mCh-Cry2olig

    Plasmid

    #101224

    Purpose

    FUS(1-214) fused to mCh-Cry2olig

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2olig

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

    Plasmid

    #85428

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-12
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCGN-ATF6 (1-373)

    Plasmid

    #27173

    Purpose

    For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

    Depositor

    Ron Prywes

    Article

    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains only aa 1-373 (please see depositor comm…

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. tol2-mpeg1-mcherry

    Plasmid

    #58935

    Purpose

    labels macrophages with mcherry

    Depositor

    Anna Huttenlocher

    Insert

    mpeg1-mcherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    mpeg1

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. JDS246

    Plasmid

    #43861

    Purpose

    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

    Depositor

    Keith Joung

    Insert

    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PG13-luc (wt p53 binding sites)

    Plasmid

    #16442

    Purpose

    Luciferase reporter containing 13 copies of the p53-binding consensus sequence

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    p53-binding site (TP53 )
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-D12L

    Plasmid

    #89161

    Purpose

    Express vaccinia virus D12L in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D12L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promotor

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Ub-G76V-GFP

    Plasmid

    #11941

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-G76V-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmiRFP670-N1

    Plasmid

    #79987

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    miRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pC015 - dLwCas13a-NF

    Plasmid

    #91905

    Purpose

    Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

    Expression

    Mammalian

    Mutation

    R474A and R1046A

    Promoter

    CAG

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCS2+IMS-HyPer7

    Plasmid

    #136469

    Purpose

    Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    SMAC/DIABLO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-hsyn-GRAB_Ado1.0

    Plasmid

    #153285

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in neurons

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBabe B-Raf V600E

    Plasmid

    #17544

    Depositor

    Channing Der

    Article

    Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

    Insert

    B-Raf (BRAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Use

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GW1-pHRed

    Plasmid

    #31473

    Purpose

    genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    pHRed
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pmGFP-ADAR2

    Plasmid

    #117929

    Purpose

    ADAR2 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR2 (ADARB1 Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pICH47751

    Plasmid

    #48002

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. NW50

    Plasmid

    #133445

    Purpose

    Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW50
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEVOL-pylT-N346A/C348A

    Plasmid

    #127411

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

    Depositor

    Wenshe Liu

    Article

    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N346A-C348A

    Promoter

    araBAD

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMK1334

    Plasmid

    #127965

    Purpose

    sgRNA expression vector compatible with CROP-Seq and imaging screens

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSET-RCaMP1h

    Plasmid

    #42874

    Purpose

    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

    Depositor

    Loren Looger

    Article

    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

    Insert

    Red Fluorescent Calcium binding Protein
    

    Use

    Tags

    EK (Enterokinase) Recognition Site, His Tag (6x),…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid

    #84561

    Purpose

    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    

    Use

    Lentiviral

    Tags

    Flag, Suntag, oxBFP, AID (auxin-inducible degron)

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEGFP-LC3

    Plasmid

    #21073

    Purpose

    Mammalian expression of rat LC3 fused to EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Promoter

    CMV

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCI-EGFP-NR2b wt

    Plasmid

    #45447

    Depositor

    Andres Barria, Robert Malinow

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR2B (Grin2b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge