163,584 results

1. pCMU-ACTLr

   Plasmid

   #61193

   Purpose

   Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   LifeAct-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROP_C9_Puro_ERBB2

   Plasmid

   #183287

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   ERBB2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EWS-GFP

   Plasmid

   #164701

   Purpose

   Expression of EWS-GFP

   Depositor

   Cheryl Arrowsmith

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. hZMPSTE24-3FLAG_pQCXIP

   Plasmid

   #89759

   Purpose

   Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

   Depositor

   Martin Dorf

   Article

   Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

   Insert

   Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
   

   Use

   Retroviral; Bicistronic expression: puromycin re…

   Tags

   9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

   Expression

   Mammalian

   Mutation

   Y253H in ZMPSTE24 (see depositor comments below)

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNCS mHoneydew

   Plasmid

   #91760

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Honeydew
   

   Use

   Tags

   no tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR4-CLIPf (w877-2)

   Plasmid

   #29650

   Depositor

   Eric Campeau

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   CLIPf

   Expression

   Mutation

   Promoter

   Available Since

   July 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFC902

   Plasmid

   #106904

   Purpose

   The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108

   Inserts

   cas9
   pyrG
   U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIG-831_HA-GD2-28z_CAR_TFAP4_Retroviral

   Plasmid

   #207508

   Purpose

   This plasmid can be used to generate retrovirus.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH6HTC_FAM98A-Halo

   Plasmid

   #175329

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   FAM98A (FAM98A Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-yoEGFP-CaURA3

    Plasmid

    #44872

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-OSF-PP

    Plasmid

    #154247

    Purpose

    backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Type

    Empty backbone

    Use

    Tags

    FLAG and Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBbi-GH

    Plasmid

    #60514

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and hygromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAd5-B1-deltaE1-GFP

    Plasmid

    #179203

    Purpose

    Block 1 with deletion in E1 and insertion of GFP expression cassette

    Depositor

    Fred Bunz

    Article

    Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

    Insert

    Modified Block 1 with deletion in E1 and insertion of GFP expression cassette
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Clover-Prx2-mRuby2

    Plasmid

    #117906

    Purpose

    fluorescent reporter of cytosolic hydrogen peroxide

    Depositor

    Hadley Sikes

    Article

    Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

    Insert

    Clover-Prx2-mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)

    Plasmid

    #70741

    Purpose

    1 color strong reporter used in study

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    sfGFP-ssrA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3n1 (572)

    Plasmid

    #70727

    Purpose

    BetI/PhlF/SrpR BCD7 3-node v41-1

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Inserts

    srpR-ssrA
    betI-ssrA
    phlF-ssrA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB-rDA2h

    Plasmid

    #208701

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA2h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. JC Mad-1

    Plasmid

    #25626

    Depositor

    Peter Howley

    Article

    Howley et al J Virol. 1980 Dec . 36(3):878-82.

    Insert

    JC Virus Mad-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

19. SL016: AAV-CAG-FLEX-CheRiff-GFP

    Plasmid

    #124776

    Purpose

    For AAV based expression of CheRiff in Cre-Positive cells

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    CheRiff-GFP
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

20. pMito-TrxRFP1

    Plasmid

    #98997

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and mitochondrial localization sequence
    

    Use

    Tags

    His-tag and Mitochondria localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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