166,169 results

1. [#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient

   Plasmid

   #233506

   Purpose

   Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

   Insert

   DsRed loxp eGFP
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.3G

   Plasmid

   #14748

   Depositor

   Christophe Benoist, Diane Mathis

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAdTrack-CMV

   Plasmid

   #16405

   Purpose

   Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-NPC1

   Plasmid

   #132295

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   NPC1 (NPC1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 14, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCasSA

   Plasmid

   #98211

   Purpose

   CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

   Depositor

   Quanjiang Ji

   Article

   Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. ppyCAG_RNaseH1_WT

   Plasmid

   #111906

   Purpose

   Express RNASEH1 in mammalian cell.

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTXB1-nbMmKappa-Tn5

   Plasmid

   #184286

   Purpose

   Anti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

   Depositor

   New York Genome Center, Ivan Raimondi

   Article

   Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

   Insert

   nbMmKappa-Tn5
   

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTXB1-nbMmIgG2a-Tn5

   Plasmid

   #184288

   Purpose

   Anti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

   Depositor

   New York Genome Center, Ivan Raimondi

   Article

   Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

   Insert

   nbMmIgG2a-Tn5
   

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. sortase A pentamutant (eSrtA) in pET29

   Plasmid

   #75144

   Purpose

   evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

   Depositor

   David Liu

   Article

   Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

   Insert

   sortase A pentamutant
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T. Deleted amino a…

   Promoter

   T7

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-GFP

    Plasmid

    #11153

    Purpose

    Mammalian expression vector for expression of GFP (CMV promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    CMV promoter-EGFP
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cre Reporter

    Plasmid

    #62732

    Purpose

    Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    LoxP-DsRED-STOP-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    LoxP sites

    Expression

    Mammalian

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-MSP1D1delltaH5

    Plasmid

    #71714

    Purpose

    Membrane scaffold protein (MSP), helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

    Plasmid

    #223747

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Lambda-PPase
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-miniTurbo

    Plasmid

    #124647

    Purpose

    Expresses Flag-miniTurbo fusion for proximity labeling

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-GfaABC1D-MCS--4x6T-WPRE

    Plasmid

    #196417

    Purpose

    Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-IRES-Puro

    Plasmid

    #85132

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFREE

    Plasmid

    #92050

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pRhamBAD and ptet

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pProEx-HTa-SOD1 WT

    Plasmid

    #191849

    Purpose

    express wild-type SOD1 in bacterial cells

    Depositor

    Nam-Chul Ha

    Article

    Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

    Plasmid

    #196703

    Purpose

    Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 D210N
    

    Expression

    Mammalian

    Mutation

    D210N, catalytically inactive

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1+-Tornado-split-nLuc

    Plasmid

    #212612

    Purpose

    For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    Tornado-CVB3-split-nLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    C373T mutation in CVB3

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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