Addgene

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pDONR223_STAT6_WT

Plasmid

#81950

Purpose

Gateway Donor vector containing STAT6, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT6 (STAT6 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-dnMCAK

Plasmid

#205993

Purpose

Over-expression of GFP-dnMCAK

Depositor

Floris Foijer

Article

Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

Insert

dnMCAK

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGL-FLKif3A

Plasmid

#13742

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

mouse Kif3A (Kif3a Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

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pBHM2404

Plasmid

#173441

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-NAT1

Use

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pUCP30T-E2Crimson

Plasmid

#78478

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTSN6

Plasmid

#131318

Purpose

Integrating the full-length tetO array (from pLAU44) near the his-3 gene in N. crassa

Depositor

Eugene Gladyshev

Article

Nguyen et al Fungal Genet Biol. 2020 Mar;136:103316.

Insert

Full-length tetO array (XbaI-NheI fragment of pLAU44)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBHM2406

Plasmid

#173442

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-HYGB

Use

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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Bmi1-overexpression

Plasmid

#21577

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

Bmi-1 (Bmi1 Mouse)

Use

Lentiviral

Tags

ires-eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-JNK1

Plasmid

#86830

Purpose

mammalian expression of GFP-JNK1

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

JNK1 (MAPK8 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAK_DR30_CasRx_GFP

Plasmid

#134843

Purpose

All in one overexpression of CasRx and guideRNA

Depositor

Michael Yaffe

Insert

RfxCas13d

Use

CRISPR

Tags

T2A EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pT2-shP53

Plasmid

#124261

Purpose

Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shP53

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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478 p5Efli1ep

Plasmid

#31160

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

chimeric fli1ep enhancer/promoter fragment

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

Plasmid

#113885

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-INPP5K

Plasmid

#171950

Purpose

Expresses INPP5K in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP5K (INPP5K Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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RFP-HA-DAT

Plasmid

#90265

Purpose

Expresses RFP-HA-DAT in mammalian cells

Depositor

Alexander Sorkin

Article

Caltagarone et al Mol Cell Neurosci. 2015 Sep;68:120-30. d

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT)

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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MAPK1

Plasmid

#73248

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MAPK1 (MAPK1 Human)

Use

Tags

His-6-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBR.DT-A

Plasmid

#35955

Depositor

Jacqueline Lees

Article

Danielian et al Oncogene. 2008 Nov 20;27(51):6561-70. Ep

Insert

diphtheria toxin A

Use

Bac recombineering

Tags

Expression

Mutation

Promoter

PGK

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pMK782 Septin6-GFP

Plasmid

#38296

Depositor

Matthew Krummel

Article

Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

Insert

Septin 6 (Septin6 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

22 amino acid linker sequence (added by PCR)

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

Plasmid

#140892

Purpose

Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.

Depositor

Yasushi Okamura

Article

Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

Insert

CiDr-VSP L223F-mCherry (tpte, vsp Ciona intestinalis, Zebrafish)

Use

Tags

mCherry

Expression

Mammalian

Mutation

changed 223L to F in Dr-VSP (zebrafish TPTE)

Promoter

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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prDNA spacer-LacOx50-20xMS2-Hyg

Plasmid

#210219

Purpose

Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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