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pLenti PGK Hygro DEST (w530-1)

Plasmid

#19066

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.Luciferase.IRES.GFP

Plasmid

#169308

Purpose

Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-EGFP

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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SpRY-Tad-CBE(CCG3)

Plasmid

#235083

Purpose

Expresses SpRY-Tad-CBE(CCG3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCG3)-SpRY Cas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ATR Kinase dead

Plasmid

#31612

Depositor

Aziz Sancar

Article

Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Mutation

K2327R (kinase dead)

Available Since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pMDG

Plasmid

#187440

Purpose

VSV-G envelope expressing plasmid

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Inserts

Expression

Mammalian

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1-FIRE-pHLy

Plasmid

#170775

Purpose

Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

Depositor

Aimee Kao

Article

Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

Insert

LAMP1 (LAMP1 Human)

Use

Lentiviral

Tags

mCherry and mTFP1

Expression

Mammalian

Mutation

84 nucleotide Signal Sequence switched to the beg…

Promoter

CMV

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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hIR-GFP

Plasmid

#22286

Depositor

Joseph Bass

Article

Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

Insert

INSR insulin receptor (INSR Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Article

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

p65 (RELA Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

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LAMP1-RpHLuorin2

Plasmid

#171720

Purpose

Lysosomal expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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1036 pcDNA3 Myr HA Akt1

Plasmid

#9008

Purpose

Mammalian expression of human Akt1 with myr, HA tags

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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Inducible Caspex expression

Plasmid

#97421

Purpose

For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

Depositor

Steven Carr, Samuel Myers

Article

Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

Inserts

Caspex
T2A-Gfp

Use

CRISPR

Tags

3xFLAG and V5

Expression

Mammalian

Mutation

dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

Promoter

TRE3G

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pLEM415-ldhL-mRFP1

Plasmid

#99842

Purpose

Expression of RFP in Lactobacillus

Depositor

Sujin Bao

Article

Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

Insert

monomeric red fluorescent protein

Tags

FLAG/Xpress

Expression

Bacterial

Promoter

ldhl

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-GalT

Plasmid

#11929

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-XBP1 mNeonGreen NLS

Plasmid

#115968

Purpose

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

X-box binding protein 1 (XBP1 Human, Synthetic)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCF153_Gag-Pol

Plasmid

#225961

Purpose

CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia Virus) Gag-Pol for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

CMV-Intron-GagPol (FMLV)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Flag-His-ATM kd

Plasmid

#31986

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM kd (ATM Human)

Tags

Flag and His

Expression

Mammalian

Mutation

3'UTRf 9 nucl/D2870A N2875k. catalytically …

Available Since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-hTERT

Plasmid

#1773

Purpose

Retroviral expression of hTERT. Used to create immortalized cell lines.

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(-30)GFP

Plasmid

#62936

Purpose

Expression of 6xHis-(-30)GFP in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMK290 (mAID-mClover-Hygro)

Plasmid

#72828

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Hygro

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERN

Plasmid

#80474

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-GFP

Plasmid

#129092

Purpose

KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

EGFP and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pZW12 (CK2beta)

Plasmid

#27088

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pPICZalphaB-SapL3

Plasmid

#78171

Purpose

Expression of Saporin L3 in pichia

Depositor

Vern Schramm

Article

Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

Insert

Saporin L3

Tags

alpha factor

Expression

Yeast

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-GFP

Plasmid

#132776

Purpose

Prime editing in mammalian cells with co-translational GFP expression

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Type

Empty backbone

Tags

6xHis, T7 tag, Xpress tag, mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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PB-GFP-Gal8

Plasmid

#127191

Purpose

Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Insert

GFP-Gal8 (LGALS8 Human)

Tags

Gal8 is fused to the c-terminus of GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

Plasmid

#89347

Purpose

2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

Jurkat TCRalpha/beta alleles and CD3e and CD3z

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 20, 2017

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER Short P2A

Plasmid

#164108

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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