We narrowed to 18,622 results for: Met
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Plasmid#104001PurposePlasmid encoding mRFP1DepositorInsertmRFP1
UseLow copyExpressionBacterialMutationMutations relative to DsRed (R2A, K5E, N6D, T21S,…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgMPC2_7
Plasmid#163457Purposelentiviral vector expressing Cas9 and an sgRNA targeting MPC2DepositorInsertsgRNA 7 targeting GPT2 (MPC2 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgMPC2_9
Plasmid#163458Purposelentiviral vector expressing Cas9 and an sgRNA targeting MPC2DepositorInsertsgRNA 9 targeting GPT2 (MPC2 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU1/Sm/3' Box_(GLuc)_INT
Plasmid#68438PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U1Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-N-miRFP670-C
Plasmid#159438PurposeCRISPR knock-in donor construct with fluorescent reporterDepositorInsertmiRFP670
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C t…PromoterCMVAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16
Plasmid#89872Purposeexpresses CB1R-iTango component in mammalian cellsDepositorInsertCB1R-V2tail-TevN-BLITz1-TetR-VP16
ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFU-cMVIIA-PE
Plasmid#24147DepositorInsertloxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)
UseLentiviralExpressionMammalianAvailable SinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Reg-L-IFP-F[2]+Venus (mammalian)
Plasmid#52903PurposeBinary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertReg-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Cat-L-IFP-F[1]+Plum (mammalian)
Plasmid#52905PurposeBinary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertCat-L-IFP-F[1]+Plum
UseRetroviralTagsL-IFP-F[1]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-tagBFP
Plasmid#65254Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
TagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-3xFlag-M694V-MEFV
Plasmid#167118PurposeGateway entry plasmid with 3xFlag-pyrin (M694V/FMF variant)DepositorInsertMEFV (MEFV Human)
UseGateway entry vectorTags3xFlagMutationM694V Methionine 694 to valine (FMF mutation)Available SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST mito roGFP2-Orp1
Plasmid#64997Purposefor expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorInsertOrp1 (HYR1 Budding Yeast)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-N-EGFP-C
Plasmid#159436PurposeCRISPR knock-in donor construct with fluorescent reporterDepositorInsertEGFP
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C t…PromoterCMVAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Clover-Nup50-N-10
Plasmid#56303PurposeLocalization: Nuclear Pore, Excitation: 505, Emission: 515DepositorAvailable SinceDec. 18, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEAST-J23101-CocE
Plasmid#114600Purpose"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"DepositorInsertCocE
UseSynthetic Biology; Cell-free protein synthesisPromoterJ23101Available SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV TBG FFluc miR122sponge
Plasmid#35657DepositorInsertmiR-122 sponge
UseAAVPromoterTBGAvailable SinceJuly 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[P4-P6(3xPP7)]
Plasmid#68432PurposeTransient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
CLPIT Cry2PHR-mCh-Rac1
Plasmid#42955DepositorInsertCry2PHR-mCherry-Rac1 (RAC1 Mustard Weed, Human)
UseRetroviralExpressionMammalianPromoterTet-Off promoterAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB822
Plasmid#47856PurposeBIOFAB reporter plasmid for measuring BBa_B1006 termination efficiencyDepositorInsertBBa_B1006 terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO11Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB803
Plasmid#47847PurposeBIOFAB reporter plasmid for measuring T7 early termination efficiencyDepositorInsertT7 early terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO2Available SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4
Plasmid#69227PurposeExpresses wild type SpCas9 fused to ZFP-TS4 in mammalian cellsDepositorInsertTS4 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-CytERM-N-17
Plasmid#56158PurposeLocalization: Endoplasmic Reticulum, Excitation: 604, Emission: 659DepositorInsertCytERM
TagsmCardinalExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A
Plasmid#140451PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A mutantDepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
sspa-GCaMP6f
Plasmid#67561Purposeshort photoactivatable fluorescent calcium reporterDepositorInsertsspa-GCaMP6f
ExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…Available SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-5
Plasmid#172760PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-5
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
XW20
Plasmid#65836PurposePunc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
ExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…Available SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
POT10-RFP
Plasmid#65326Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT8-RFP
Plasmid#65324Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DARPinOFF7
Plasmid#182397PurposeBacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusionDepositorInsertVNp-DARPinOFF7
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
POT7-RFP
Plasmid#65323Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT6-RFP
Plasmid#65322Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
M-ST1-VPR
Plasmid#68498Purposenuclease competent ST1-Cas9 fused to VPRDepositorInsertST-Cas9-VPR
TagsVPRExpressionMammalianPromoterCMVAvailable SinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFU-iMVIIA-PE
Plasmid#24146DepositorInsertomega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP-IRES-TetR-KRAB (PDGFRB Human, Conus magus, Aequorea victoria)
UseLentiviralExpressionMammalianAvailable SinceApril 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
spa-GCaMP6f
Plasmid#67558Purposephotoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
TagsHIS-tagExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterCMVAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV_mIFP IRES EGFP
Plasmid#72443PurposeExpressing mIFP and EGFP in mammalian cellsDepositorInsertmIFP T2A HO1 IRES EGFP
UseLentiviralExpressionMammalianAvailable SinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
Plasmid#52906PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)
Plasmid#52907PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]+Plum
UseRetroviralTagsL-IFP-F[1]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2
Plasmid#69225PurposeExpresses wild type SpCas9 fused to ZFP-TS2 in mammalian cellsDepositorInsertTS2 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCarCBA
Plasmid#117754PurposeCodon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
ExpressionBacterialPromoterPLac(UV5)Available SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
mIFP-CytERM-N-17
Plasmid#56217PurposeLocalization: Endoplasmic Reticulum, Excitation: 683, Emission: 704DepositorInsertCytERM
TagsmIFPExpressionMammalianPromoterCMVAvailable SinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mIFP-Paxillin-22
Plasmid#56234PurposeLocalization: Focal Adhesions, Excitation: 683, Emission: 704DepositorInsertPaxillin
TagsmIFPExpressionMammalianPromoterCMVAvailable SinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
POT11-RFP
Plasmid#65327Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS424-URR
Plasmid#65330Purposehigh copy number plasmid as receiving vector for exogenous pathwayDepositorInsertURR1-RFP-Leu2-URR2
UseSynthetic BiologyAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT5-RFP
Plasmid#65321Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS414-URR
Plasmid#65329PurposeCEN plasmid as receiving vector for exogenous pathwayDepositorInsertURR1-RFP-Leu2-URR2
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMV-URR2
Plasmid#65332Purposestandard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUsDepositorInsertURR2
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only