We narrowed to 5,067 results for: codon optimized

1. HDM_RSV_B_1992_F

   Plasmid

   #237358

   Purpose

   Expression plasmid for RSV B F from 1992

   Depositor

   Jesse Bloom

   Article

   Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

   Insert

   RSV B F from 1992 human codon optimized from Genbank Accesion OK649748
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HDM_RSV_B_1982_F

   Plasmid

   #237357

   Purpose

   Expression plasmid for RSV B F from 1982

   Depositor

   Jesse Bloom

   Article

   Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

   Insert

   RSV B F from 1982 human codon optimized from Genbank Accesion MG642043
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HDM_RSV_B_2019_F

   Plasmid

   #237359

   Purpose

   Expression plasmid for RSV B F from 2019

   Depositor

   Jesse Bloom

   Article

   Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

   Insert

   RSV B F from 2019 human codon optimized from Genbank Accesion PP352433
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HDM_RSV_B1_G_31AACTdel

   Plasmid

   #237354

   Purpose

   Expression plasmid for RSV B1 G

   Depositor

   Jesse Bloom

   Article

   Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

   Insert

   RSV B1 G human codon optimized from Genbank Accesion AF013254
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   31 amino acid cytoplasmic tail deletion

   Promoter

   CMV

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9-humanized

   Plasmid

   #44246

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dead Cas9 with 3X NLS
   

   Use

   CRISPR and Retroviral; Vector

   Tags

   3x NLS

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. R4pGWB601_UBQ10p-Turbo-YFP-NLS

   Plasmid

   #127368

   Purpose

   Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Plant

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Arabidopsis UBQ10 promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIP hIL2 co

   Plasmid

   #90513

   Purpose

   Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   IL2 (IL2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. evoBxb1_IVT

   Plasmid

   #222341

   Purpose

   Plasmid for evolved Bxb1 mRNA in vitro transcription template

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved Bxb1
   

   Use

   Template for in vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBUN421

   Plasmid

   #62204

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   TaU3 and Ubi

   Available since

   Feb. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET151-GyrA

    Plasmid

    #216229

    Purpose

    S. aureus USA300 GyrA Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid

    #127370

    Purpose

    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCA24N-sso7d-ligase

    Plasmid

    #87745

    Purpose

    IPTG-inducible expression of sso7d-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and codon optimized sso7d (Sulfolobus solfa…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-MH6-RspCas13d_RspCasWYL1

    Plasmid

    #108304

    Purpose

    Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Inserts

    RspCas13d
    RspWYL1
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid

    #104909

    Purpose

    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET151-GyrB

    Plasmid

    #216233

    Purpose

    S. aureus USA300 GyrB Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. wtBxb1_IVT

    Plasmid

    #222340

    Purpose

    Plasmid for wild type Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wtBxb1
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7 (inactivated)

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

    Plasmid

    #87259

    Purpose

    YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133

    Depositor

    David Sibley

    Article

    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

    Inserts

    YFP-mAID-3HA
    HXGPRT
    

    Use

    Toxoplasma expression

    Tags

    3HA and mAID

    Expression

    Mutation

    Codon optimized for Toxoplasma gondii expression

    Promoter

    TgDHFR-TS 463 bp and TgTUB1 378 bp

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid

    #127354

    Purpose

    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPR-mcBEST

    Plasmid

    #209416

    Purpose

    Plasmid for multiplexed cytosine base editing in streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

    Inserts

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    base editing cassette: PtipA ; sgRNAs: PkasO* an…

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-BaLgp120-8his

    Plasmid

    #100925

    Purpose

    Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Use

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCas9-EcRT-GAL-Hyg

    Plasmid

    #232091

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Lenti-AsCpf1-Blast

    Plasmid

    #84750

    Purpose

    Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    AsCpf1
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-3xHA (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MO91-dCas9-AID(P182X)

    Plasmid

    #83260

    Purpose

    Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

    Depositor

    Xing Chang

    Article

    Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

    Insert

    dCas9-AID P182X
    

    Use

    Retroviral

    Tags

    3xFLAG-NLS and FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9; P182X in AID

    Promoter

    MSCV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

24. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

    Plasmid

    #45574

    Purpose

    Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    co I-Sce I
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR_L1-Turbo-YFP-NLS-STOP-L2

    Plasmid

    #127361

    Purpose

    gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENO1-BFP-NAT

    Plasmid

    #50046

    Purpose

    Expression of BFP in Candida albicans

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    BFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pENO1

    Available since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSH-EFIRES-P-AtAFB2

    Plasmid

    #129715

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    no

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDF0352 HvsCas7-11 with C-term Nes mammalian expression

    Plasmid

    #172516

    Purpose

    Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    C-term Nes HvsCas7-11 mammalian expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCEP4-BG505.SOSIP-8his

    Plasmid

    #111847

    Purpose

    Mammalian expression plasmid for soluble BG505 SOSIP.664

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 Env (BG505 SOSIP.664)
    

    Use

    Tags

    8his purification tag and CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; has SOSIP mutatio…

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTE4396

    Plasmid

    #74041

    Purpose

    Expresses human codon-optimized AsCpf1 and As crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    As crRNA
    AsCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAVS1-Blasticidin-CAG-Flpe-ERT2

    Plasmid

    #68461

    Purpose

    AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    mammalian-codon-optimized Flpe recombinase fused with ERT2
    

    Use

    Synthetic Biology

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDNR-SpCas9-Cdt1

    Plasmid

    #80426

    Purpose

    Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Cdt1
    

    Use

    CRISPR

    Tags

    Cdt1

    Expression

    Mutation

    codon optimized for human

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid

    #127353

    Purpose

    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SpCas9-NRTH

    Plasmid

    #198541

    Purpose

    Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRTH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-HCMVgL-FLAG (TB40/E)

    Plasmid

    #136449

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein L (UL115 Human betaherpesvirus 5)
    

    Use

    Tags

    Short SGGSG linker and FLAG tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCEP4-DU422gp140-TM

    Plasmid

    #123251

    Purpose

    Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (DU422) Env
    

    Use

    Tags

    CD5 leader peptide and Linker (with 6his tag) and…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pENTR_L1-YFP-miniTurbo-NES-L2

    Plasmid

    #127351

    Purpose

    gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

    Plasmid

    #127369

    Purpose

    Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCas9-EcRT-GAL-HIS3

    Plasmid

    #232093

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCas9-EcRT-GAL-Nat

    Plasmid

    #232090

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge