We narrowed to 5,553 results for: pcDNA3.1+

Showing: 3751 - 3800 of 5553 results

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pDN34

Plasmid

#61343

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS2 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A

Plasmid

#41999

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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MCP/PCP RNA Lantern

Plasmid

#226095

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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WNK2 WT

Plasmid

#24569

Depositor

Joseph Costello

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

Insert

WNK2 (WNK2 Human)

Tags

His6 and V5

Expression

Mammalian

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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pSL2783 (pTnsC-VP64_Vch)

Plasmid

#200896

Purpose

Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsC-VP64

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-PhoCl-mCherry-myc

Plasmid

#87692

Purpose

Express PhoCl-mCherry with C-term myc tag in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl-mCherry-myc

Tags

myc tag

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NP

Plasmid

#74737

Purpose

mammalian expression of the Nucleoprotein of the A/New Caledonia/20/99 (H1N1) Influenza virus

Depositor

Elisa Vicenzi

Article

Di Pietro et al J Virol. 2013 Apr;87(8):4523-33. doi: 10

Insert

Nucleoprotein

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAL149

Plasmid

#22275

Purpose

Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PhyB(1-908) (PHYB Mustard Weed)

Use

Synthetic Biology

Tags

mCherry-Kras4BCT (prenylation sequence)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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mVenus-IQ6 WT

Plasmid

#198201

Purpose

FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Tags

mVenus

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-V1-ORF

Plasmid

#73635

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-V2-ORF

Plasmid

#73636

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-V2

Plasmid

#73638

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-DRD2

Plasmid

#24099

Depositor

Jean-Michel Arrang

Article

Jeanneteau et al Mol Biol Cell. 2004 Feb . 15(2):696-705.

Insert

DRD2 (DRD2 Human)

Tags

alpha7 nicotinic signal peptide and eGFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-V1

Plasmid

#73637

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pER-eZinCh-2

Plasmid

#70009

Purpose

expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

Depositor

Maarten Merkx

Article

Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

Insert

ER-eZinCh-2

Tags

KDEL sequence and PPI signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MEKK1 WT

Plasmid

#12181

Depositor

Gary Johnson

Article

Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Insert

MEKK1 (Map3k1 Mouse)

Tags

His and Xpress

Expression

Mammalian

Mutation

V1467L

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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Halo-NEMO

Plasmid

#187899

Purpose

Mammalian cell expression of Halo-tagged NEMO

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

NEMO (Ikbkg Mouse)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-Smad4M

Plasmid

#14959

Depositor

Joan Massague

Article

Kang et al Proc Natl Acad Sci U S A. 2005 Sep 27. 1

Insert

Smad4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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mcherry Beta 1

Plasmid

#36111

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B1 (GNB1 Bovine)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
FL-mCherry

Plasmid

#78862

Purpose

DULIP negative control (prey) to exclude unspecific background binding

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

mCherry

Use

Expression vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pXeDNA3.1-LacZ

Plasmid

#242746

Purpose

pXeDNA3-LacZ TA-cloning vector for dual expression: CMV enhancer/promoter in mammalian cells and Xenopus β-globin 5'/3'UTRs in oocytes; BGHpA, AmpR, NeoR, lacZα for blue-white screening.

Depositor

Luis Yanez-Guerra

Article

Guan et al MicroPubl Biol. 2025 Dec 1;2025:10.17912

Type

Empty backbone

Use

Xenopus oocyte expression

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-Nedd4/2795

Plasmid

#11426

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

D661N compared to GenBank reference sequence U966…

Promoter

CMV

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-mZO-1(9-1745)-Myc-His

Plasmid

#236529

Purpose

For expression of ZO-1 (9-1745 aa, mouse)-Myc-His alpha+ in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al Tissue Barriers. 2019;7(3):e1653748. doi

Insert

TJP1 (Tjp1 Mouse)

Tags

GFP and Myc-His

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S0-mRuby2-Vt

Plasmid

#233226

Purpose

Vinculin FRET tension sensor with coiled-coil S0 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S0-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vinculin-Clover-CC-S0-mRuby2

Plasmid

#233233

Purpose

Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S0-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Rod Opsin

Plasmid

#109361

Purpose

Human rod opsin

Depositor

Robert Lucas

Article

Bailes et al Proc Biol Sci. 2013 Apr 3;280(1759):2012

Insert

Rod opsin (RHO Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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perbB2-ECFP

Plasmid

#40268

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB2 (ERBB2 Human)

Tags

ecfp

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Hexim2 (P#1897)

Plasmid

#14663

Depositor

Matija Peterlin

Article

Barboric et al EMBO J. 2005 Dec 21. 24(24):4291-303.

Insert

Hexim2 (HEXIM2 Human)

Tags

His and Xpress

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

Plasmid

#188994

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-CD28TMD-NTEVp(H75S)

Plasmid

#137828

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(H75S)

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Histidine 75 to Serine in NTEVp fragment

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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SP-GFP-CD274-UTR

Plasmid

#121142

Purpose

Mammalian expression of GFP tagged CD274 with its own 3'UTR

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

CD274 with its own 3'UTR (CD274 Human)

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHLuc (Antares-SEPLuc C1A4E+6)

Plasmid

#141089

Purpose

final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-3xFLAG-TCAB1

Plasmid

#51630

Purpose

Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

TCAB1 (WRAP53 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
CMV-SpyCas9

Plasmid

#113033

Purpose

SpyCas9 expression in mammalian cells (CMV promoter driven)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SpyCas9

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 MEKK1 KD

Plasmid

#12180

Depositor

Gary Johnson

Article

Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Insert

MEKK1 (Map3k1 Mouse)

Tags

His and Xpress

Expression

Mammalian

Mutation

Kinase dead: K1253M; V1467L

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
Myc-Bnip3FL

Plasmid

#100796

Purpose

Mammalian expression of Bnip3 full length splice variant

Depositor

Joseph Gordon

Article

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mus Bnip3 (Bnip3 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p4B5-EGFP

Plasmid

#140324

Purpose

Detection of Dengue virus infection in live cells

Depositor

Alan Rothman

Article

Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

Insert

DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP

Tags

eGFP

Expression

Mammalian

Mutation

2 conservative n.t. differences near 5' end …

Promoter

CMV

Available Since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV2-hNCOA6:V5

Plasmid

#63585

Purpose

A subunit of the Trithorax-related (Trr) methyltransferase complex.

Depositor

Kenneth Irvine

Article

Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

Insert

NCOA6 (NCOA6 Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pGP-CMV-jGCaMP8f F366H

Plasmid

#204141

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Phenylalanine 366 changed to histidine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-CyclinF-ΔF

Plasmid

#32973

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Tags

FLAG

Expression

Mammalian

Mutation

F-box mutations: L35A and P36A

Promoter

CMV

Available Since

Feb. 3, 2012

Availability

Academic Institutions and Nonprofits only
WN10151

Plasmid

#80441

Purpose

Expresses human codon-optimized dead (832A) LbCpf1

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Insert

dead (D832A) LbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

D832A

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pmEGFP-C1-Nir3-LNS2

Plasmid

#220087

Purpose

C-terminal PA binding domain of Nir3

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM2 (PITPNM2 Human)

Tags

mEGFP-GGGGSHM

Expression

Mammalian

Mutation

amino acids 925-1209

Promoter

CMV

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hHSF1 K80Q

Plasmid

#118351

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80Q

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Smac(1-60)mCherry

Plasmid

#40880

Depositor

Douglas Green

Article

Tait et al Dev Cell. 2010 May 18;18(5):802-13.

Insert

Smac(1-60)mCherry (DIABLO Human)

Tags

mCherry

Promoter

CMV

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
LIMK1 T508EE

Plasmid

#245950

Purpose

Expressing constitutively active mimetic of phospho-Thr508 LIM Kinase 1

Depositor

Gordon Gill

Article

Edwards et al J Biol Chem. 1999 Apr 16;274(16):11352-6

Insert

LIM domain kinase 1 (Limk1 Mouse)

Expression

Mammalian

Mutation

T508EE (insert of 2 glutamates)

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-CBS (WT)

Plasmid

#182923

Purpose

Mammalian expression plasmid for human CBS

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

Cystathionine Beta-Synthase (CBS Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-casp9_AtCRY2-PHR-casp9

Plasmid

#131221

Purpose

Expresses Opto-casp9_AtCRY2-PHR-casp9 (light-activated caspase9)

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

Opto-casp9 (AtCRY2-PHR-casp9)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HA-Nedd4(C744E)/2797

Plasmid

#11206

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

cysteine 744 mutated to glutamic acid

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pFH2.30_Gag-MCP-dpol-T2A-eGFP

Plasmid

#205538

Purpose

Express HIV Gag-MCP-dpol-T2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

HIV Gag-MCP-dpol

Expression

Mammalian

Mutation

pol frameshift sequence recoded

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 5,553 results for: pcDNA3.1+