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pET28a-MSP1D1delltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pF151 pcDNA3.1(+)SOD1WT

Plasmid

#26397

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAG153 FKBP-CFAST11

Plasmid

#130813

Purpose

Expresses FKBP-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FKBP-CFAST11

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-GCN5

Plasmid

#65386

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

GCN5 (KAT2A Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pSBbi-RB

Plasmid

#60522

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.Cre.WPRE.hGH

Plasmid

#105550

Purpose

AAV expression of Cre from GFAP promoter

Depositor

James M. Wilson

Has Service

AAV PHP.eB and AAV5

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

GFAP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-GFP-FLAG-cGAS

Plasmid

#86675

Purpose

Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

GFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-RILP

Plasmid

#110498

Purpose

Fluorescent RILP

Depositor

Terence Dermody, Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

RILP (RILP Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCXB-EBNA1

Plasmid

#41857

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Expression

Mammalian

Promoter

CAG

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pXG260

Plasmid

#141282

Purpose

CMV:EGFP (as control for pXG237)

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

EGFP

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 19, 2020

Availability

Academic Institutions and Nonprofits only

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T7-TPase

Plasmid

#51818

Purpose

For in vitro transcription of Tol2 transposase using T7 Promoter

Depositor

Elly Tanaka

Article

Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

Insert

Tol2 Transposase

Use

Unspecified

Promoter

T7

Available Since

April 10, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-CD36-C-10

Plasmid

#55011

Purpose

Localization: Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

CD36 (CD36 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH RAP80

Plasmid

#27501

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

RAP80 (UIMC1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only

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mCherry-Vinculin-23

Plasmid

#55159

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Vinculin (VCL Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176269

Purpose

Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCasMINI-V4-VPR-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Luc

Plasmid

#45968

Depositor

Ralf Kuehn

Insert

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP_TRPM8

Plasmid

#64879

Purpose

Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

Depositor

Sebastián Brauchi

Article

Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

Insert

Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)

Tags

eGFP

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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PKC iota WT

Plasmid

#21252

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC iota (PRKCI Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pCAX APP 695

Plasmid

#30137

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Expression

Mammalian

Mutation

wild type splice variant 695

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST PKAKTRClover

Plasmid

#59153

Purpose

Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA (PRKACA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pME-ERT2-Cre-ERT2

Plasmid

#82587

Purpose

Middle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombination

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

ERT2-CRE-ERT2

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCytochrome C-GFP

Plasmid

#41182

Depositor

Douglas Green

Article

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_PGK

Plasmid

#79120

Purpose

Lentiviral vector for constitutive expression of genes (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV4 p65

Plasmid

#21966

Depositor

Warner Greene

Article

Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

Insert

NFkB p65 subunit (RELA Human)

Expression

Mammalian

Mutation

This construct was the product of 3 subcloning pr…

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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COM-epegRNA-Dnmt1

Plasmid

#211376

Purpose

Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

COM-epegRNA

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-OSKM

Plasmid

#20321

Purpose

Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pKeima-LiveDrop

Plasmid

#199695

Purpose

lipophagy reporter system based on the Keima-fluorophore

Depositor

Tobias Walther

Article

Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

Insert

GPAT4 (Gpat4 Fly)

Tags

Keima

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#42875

Purpose

A crRNA expression plasmid for targeting a specific sequence.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR-BsaI

Use

CRISPR; E.coli

Expression

Bacterial

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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8,964 results