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Showing: 4051 - 4100 of 13557 results

  7. p7SL-neo-TET

    Plasmid
    #51285
    Purpose
    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    7SL RNA (RN7SL2 Human)
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    7SL upstream pollII enhancer
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51285-plasmid-map-sequence-id-78367

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  11. pRN3P_T3_ABE8e_IVT

    Plasmid
    #201676
    Purpose
    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.
    Depositor
    Timo Otonkoski
    Article
    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR; Vector for in-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T3 promoter
    Available since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    201676-plasmid-map-sequence-id-406944

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  12. pU6-neo-TET

    Plasmid
    #51286
    Purpose
    expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    U6 (RNU6-1 Human)
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    U6 upstream enhancer sequence
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51286-plasmid-map-sequence-id-78368

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  17. p420-3

    Plasmid
    #71257
    Purpose
    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Article
    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.
    Insert
    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    GM-CSF minimal
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71257-plasmid-map-sequence-id-131889

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  21. pSW002-Pc-TorA(sp)-mTurquoise2

    Plasmid
    #111254
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorA(sp)-mTurquoise2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mTurquoise2 is codon optimized for expression in …
    Promoter
    Pc
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111254-plasmid-map-sequence-id-215265

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  41. pCSN068

    Plasmid
    #101749
    Purpose
    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101749-plasmid-map-sequence-id-202306

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  49. pET20b_Pa-PirA

    Plasmid
    #128956
    Purpose
    expresses Pseudomonas aeruginosa PirA in E. coli
    Depositor
    James Naismith
    Article
    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24
    Insert
    ferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
    Use
    Tags
    6xHis and pelB leader
    Expression
    Bacterial
    Mutation
    Signal peptide sequence has been removed
    Promoter
    T7
    Available since
    Aug. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128956-plasmid-map-sequence-id-254679

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Showing: 4051 - 4100 of 13557 results