4,073 results

1. CLC-pmCherryC1

   Plasmid

   #27680

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Clathrin Light Chain (Clta Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA3.1-hMcl-1

   Plasmid

   #25375

   Depositor

   Roger Davis

   Article

   Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

   Insert

   MCL1 (MCL1 Human)
   

   Use

   Tags

   V5 and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-CibN-dCas9-CibN

   Plasmid

   #60553

   Purpose

   Expresses CibN-dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CKAR

   Plasmid

   #14860

   Depositor

   Alexandra Newton

   Article

   Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

   Insert

   C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCFJ601 - Peft-3 Mos1 transposase

   Plasmid

   #34874

   Purpose

   Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   Mos1 transpoase
   

   Use

   Worm targeting

   Tags

   Expression

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. dxCas(3.7)-VPR

   Plasmid

   #108383

   Purpose

   Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   dxCas(3.7)-VPR
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Stat3 Flag pRc/CMV

   Plasmid

   #8707

   Depositor

   Jim Darnell

   Article

   Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

   Insert

   Stat3 (Stat3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   *see comments

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3 CDK9 HA (P#635)

   Plasmid

   #14640

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

   Insert

   cdk9 (CDK9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmTurquoise2-N1

   Plasmid

   #60561

   Purpose

   Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLIK-Zeo

    Plasmid

    #25736

    Purpose

    Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHD-DsRed

    Plasmid

    #51434

    Purpose

    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

    Depositor

    Kate O'Connor-Giles

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    LoxP-3xP3-DsRed-LoxP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    3xP3

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HDM-SARS2-Spike-delta21

    Plasmid

    #155130

    Purpose

    Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized to H. sapiens using IDT codon opt…

    Promoter

    CMV

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-SADB19G

    Plasmid

    #32633

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    SADB19G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 4xM67 pTATA TK-Luc

    Plasmid

    #8688

    Depositor

    Jim Darnell

    Article

    Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

    Insert

    4 repeated M67 sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 Hygro HA Akt2

    Plasmid

    #16000

    Depositor

    Morris Birnbaum

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-Xiap-Myc

    Plasmid

    #11833

    Depositor

    Guy Salvesen

    Article

    Takahashi et al J Biol Chem. 1998 Apr 3. 273(14):7787-90

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FLAG-KD LKB1

    Plasmid

    #8591

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K78I: lysine 78 mutated to isoleucine; kinase dead

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-N1-ACTR3

    Plasmid

    #8462

    Depositor

    Matthew Welch

    Article

    Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

    Insert

    ACTR3 (ACTR3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-HIF2alpha-P405A/P531A-pcDNA3

    Plasmid

    #18956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP-NMHC II-C

    Plasmid

    #10843

    Depositor

    Robert Adelstein

    Article

    Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

    Insert

    NMHC II-C (Myh14 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV.PGK.mFoxa2

    Plasmid

    #33014

    Depositor

    Malin Parmar

    Article

    Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

    Insert

    Forkhead box A2 (Foxa2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H10L

    Promoter

    Available since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGFP(C3)-Vinculin

    Plasmid

    #30312

    Depositor

    Klaus Hahn

    Insert

    Vinculin (Vcl Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    F633S, V828E, L936P in mouse viniculin (NM_009502…

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3.1- dCas9-2xNLS-EGFP

    Plasmid

    #74710

    Purpose

    Expresses dCas9-2xNLS-EGFP in mammalian cells

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    dCas9-2xNLS-EGFP
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid

    #73796

    Purpose

    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

    Depositor

    Ronald Davis

    Article

    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

    Inserts

    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1, pRPR1(TetO), and pTef1

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HBT-pcoCas9

    Plasmid

    #52254

    Purpose

    transient expression of pcoCas9 gene in plant cells

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    pcoCas9
    

    Use

    CRISPR; Plant expression

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    hybrid constitutive promoter 35SPPDK

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPAmCherry-b-actin

    Plasmid

    #31949

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    b-actin

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SepOTSλ

    Plasmid

    #68292

    Purpose

    Phosphoserine orthogonal translation system (preferred variant)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EFSep21, 4xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid

    #70684

    Purpose

    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.

    Depositor

    Bob Goldstein

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPAmCherry1-C1

    Plasmid

    #31929

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. FLAG.PKCepsilon

    Plasmid

    #10795

    Depositor

    Alex Toker

    Article

    Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

    Insert

    PKC epsilon (PRKCE Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAKgfp1

    Plasmid

    #14076

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

33. pAAV-GFAP104-mCherry

    Plasmid

    #58909

    Purpose

    AAV-mediated expression of mCherry under the GFAP promoter

    Depositor

    Edward Boyden

    Article

    Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

    Has Service

    AAV5

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMVTAG-NEMO

    Plasmid

    #11970

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMO (IKBKG Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Emerin pEGFP-C1 (637)

    Plasmid

    #61993

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Emerin (EMD Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-HDAC6.DC-FLAG

    Plasmid

    #30483

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Catalytically inactive mutant (H216/611A)

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFOS WT-GL3

    Plasmid

    #11983

    Purpose

    Reporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.

    Depositor

    Ron Prywes

    Article

    Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

    Insert

    c-fos promoter (Fos Mouse)
    

    Use

    Tags

    Luc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHIV-H2BmRFP

    Plasmid

    #18982

    Depositor

    Zena Werb, Bryan Welm

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Insert

    Histone H2B monomeric red fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GFP Snail WT

    Plasmid

    #16225

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pC1-HyPer-Red-C199S

    Plasmid

    #48252

    Purpose

    Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED-C199S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge