We narrowed to 25,909 results for: Nes
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Plasmid#98696PurposeI-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafishDepositorInsertPDGFRA (PDGFRA Human)
UseZebrafish expressionAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-MFSD8-Puro
Plasmid#239954PurposeLentiviral vector to generate MFSD8 stable expressing cell line under CMV promoterDepositorInsertMFSD8
UseLentiviralTagsmyc-flagExpressionMammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-Bar-U-MatryoshCaMP6s
Plasmid#100024PurposePlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
ExpressionPlantPromoterAtUBQ10Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p(A)n-PAUSE
Plasmid#118068PurposeTranscription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin geneDepositorInsertSynthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
UseSynthetic BiologyExpressionMammalianAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAEL005635-Cas9
Plasmid#100608PurposeThe promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL005635-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL005635 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CR-QUAS:NTR-mKate2
Plasmid#241914PurposeZebrafish transgenesis plasmid encoding nitroreductase-mKate2, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis markerDepositorInsertQUAS enhancer driving nitroreductase-mKate2
UseZebrafish tol2 transgenesisTagsnitroreductase fused with mKate2PromoterQUASAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OASL-V5 puro
Plasmid#158643PurposeLentiviral expression vector for constitutive expression of V5-tagged OASL.DepositorAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OAS1-V5 puro
Plasmid#158641PurposeLentiviral expression vector for constitutive expression of V5-tagged OAS1.DepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1
Plasmid#70145Purposemammalian expression of MKLP1 with an eGFP fusionDepositorAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
Tags6xHistidine and HiBiTExpressionBacterialPromoterT7Available SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant
Plasmid#101209PurposeBacterial expression plasmid for SpCas9-HF1 variantDepositorInsertSpCas9 variant N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationN497A, R661A, Q695A and Q926APromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-STAT1
Plasmid#82722PurposeExpresses human STAT1DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB3alpha2_35S:P19:Tnos (GB1203)
Plasmid#68214PurposeTranscriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoterDepositorInsertP19 silencing suppressor
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:FLuc
Plasmid#118063PurposeTranscriptional unit for constitutive expression of FLuc in mammalian cellsDepositorInsertCMV:FLuc:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:RedF
Plasmid#118064PurposeTranscriptional unit for constitutive expression of RedF in mammalian cellsDepositorInsertCMV:RedF:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoterCMVAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
p35S:Citrine-TurboID-3xHA
Plasmid#199243PurposeThis plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.DepositorInsertCitrine-TurboID
Tags3xHA and TurboID-3xHAExpressionPlantPromoterCaMV 35SAvailable SinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-T7-hOsa2
Plasmid#17987DepositorAvailable SinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
YEE-BE4max
Plasmid#138157PurposeC-to-T base editorDepositorInsertrAPOBEC1(YEE)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E + R132E; within Cas9…PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_G299C
Plasmid#177829PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_G299C
ExpressionMammalianMutationG299CPromoterCMVAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-MKK6-EE
Plasmid#82718PurposeExpresses constitutively active human MKK6. Multiple cloning site 2 is empty.DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF-BOS-RIG-I-Myc
Plasmid#215872PurposeRIG-I-Myc expression vector for overexpressionDepositorInsertRIG-I (RIGI Human)
ExpressionMammalianAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xP53_RE::NLuc
Plasmid#124533Purposep53 NLuc luciferase reporterDepositorInsertP53 NLuc reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUBQ:Citrine-TurboID-3xMyc
Plasmid#199244PurposeThis plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.DepositorInsertCitrine-TurboID-3xMyc
TagsTurboID-3xMycExpressionPlantPromoterArabidopsis UBQ10 promoter with intronAvailable SinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
5xNF-kb_RE::RedF
Plasmid#124530PurposeNFKB RedFirefly luciferase reporterDepositorInsertNF-κβ RedF reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgSlc17a7
Plasmid#124846PurposeMutagenesis of Slc17a7DepositorInsertSlc17a7 (Slc17a7 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-trGluc
Plasmid#158959PurposeDNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).DepositorInserttrGluc
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB2904(XI-3 MarkerFree)
Plasmid#73276PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:dCas9:nls:10P3
Plasmid#135982PurposeExpresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cellsDepositorInsertdCas9 fused to 10 copies of the P3 coiled-coil
UseCRISPRTagsHisExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MEK1-DD
Plasmid#47576PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1DepositorInsertMEK1-DD (Map2k1 Mouse)
Tags3xHA and GSTExpressionBacterialMutationSer218 to Asp, Ser221 to AspAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-CREB
Plasmid#68550PurposeAAV expression of CREBDepositorInsertCREB
UseAAVTagsEGFPExpressionMammalianPromoterCMVAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE 3xHA-MKK6-EE puro
Plasmid#216791PurposeMammalian expression of constitutively active human MKK6DepositorInsertMKK6 (MAP2K6 Human)
UseRetroviralTags3x-HAExpressionMammalianMutationS207E and T211E, constitutively activePromoterLTRAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-mCREB
Plasmid#68551PurposeAAV expression of mCREBDepositorInsertmCREB
UseAAVTagsEGFPExpressionMammalianMutationS133APromoterCMVAvailable SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-JNK1a1
Plasmid#47574PurposeBacterial expression plasmid for GST-3xFlag-tagged JNK1a1DepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Acd82
Plasmid#174100PurposeAcridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertAcridone 82 tRNA synthetase and tRNA
ExpressionBacterialMutationN311S, C313G, V366A, W382TAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-maxi
Plasmid#196337PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRedF
Plasmid#118057PurposeGBPart - RedF Luciferase CDSDepositorInsertRed Firefly Luciferase CDS
UseSynthetic Biology; Domestication of dna parts for…MutationRed mutant S286YAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEasyCas9
Plasmid#184909PurposeExpresses the Streptococcus pyogenes Cas9 proteinDepositorInsertCas9
UseCRISPRExpressionBacterial and YeastAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
AALN-BE4max
Plasmid#138161PurposeC-to-T base editorDepositorInsertrAPOBEC1(AALN)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 R33A + K34A + H122L + D124N; with…PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX302-MKP5-V5 puro
Plasmid#87770PurposeExpresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)DepositorAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHLuc (Antares-SEPLuc C1A4E+6)
Plasmid#141089Purposefinal optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E+6
ExpressionMammalianPromoterCMVAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shGATA6 puro
Plasmid#72615PurposeExpresses an inducible short hairpin targeting human GATA6 sequenceDepositorInsertshort hairpin GATA binding protein 6
UseLentiviralTagsNoneExpressionMammalianPromoterTRE TightAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Myc-Bnip3FL
Plasmid#100796PurposeMammalian expression of Bnip3 full length splice variantDepositorAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-MAPKAPK2
Plasmid#82720PurposeExpresses human MAPKAPK2DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
NEK7_HUMAN_D0
Plasmid#79735PurposeThis plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_A215C
Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
ExpressionMammalianMutationA215CPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:NLuc
Plasmid#118065PurposeTranscriptional unit for constitutive expression of NLuc in mammalian cellsDepositorInsertCMV:NLuc:bGH
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3
Plasmid#111924PurposeOrange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μDepositorInsertOeNL(Ca2+)_18μ
TagsCoxVIIIx2ExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
TNPO1-shRNA
Plasmid#86082PurposeA lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.DepositorAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MKK6-EE
Plasmid#47579PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6DepositorInsertMKK6-EE (MAP2K6 Human)
Tags3xHA and GSTExpressionBacterialMutationSer207 to Glu, Thr211 to GluAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only