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Showing: 3561 - 3600 of 158338 results

  9. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    128605-plasmid-map-sequence-id-318004

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  12. pX330.puro

    Plasmid
    #110403
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110403-plasmid-map-sequence-id-213570

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  14. pJR104

    Plasmid
    #187243
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187243-plasmid-map-sequence-id-374823

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  19. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid
    #29663
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    6xHis, GFP, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29663-plasmid-map-sequence-id-197189

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  20. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

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  30. pETMCSI-T4P_Y320A

    Plasmid
    #122522
    Purpose
    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis
    Depositor
    Gottfried Otting
    Article
    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do
    Insert
    gp43 - DNA polymerase (43 Escherichia virus T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tyrosine 320 changed to alanine
    Promoter
    T7 promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122522-plasmid-map-sequence-id-238200

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  31. PB-TO-hNIL BSD-mApple

    Plasmid
    #182313
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    bsd-T2A-mycNLS-mApple
    Use
    Tags
    T2A-mycNLS-mApple
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182313-plasmid-map-sequence-id-359511

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  32. p5154

    Plasmid
    #175390
    Purpose
    High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)
    Depositor
    Michael Wiles
    Article
    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10
    Type
    Empty backbone
    Use
    Mouse Targeting; Cloning vector, to be used to de…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    175390-plasmid-map-sequence-id-348371

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  34. pcDNA3-CFP

    Plasmid
    #13030
    Purpose
    Mammalian expression vector for cloning your gene fused to CFP
    Depositor
    Doug Golenbock
    Has Service
    Cloning Grade DNA
    Insert
    cyan fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13030-plasmid-map-sequence-id-212096

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  35. pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

    Plasmid
    #233036
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    233036-plasmid-map-sequence-id-465997

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  36. pCas9-EcRT-GAL-Hyg

    Plasmid
    #232091
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232091-plasmid-map-sequence-id-462304

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Showing: 3561 - 3600 of 158338 results