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Krox20 promoter pGL3-TATA

Plasmid

#21260

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

Krox20 promoter (Egr2 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-pGK-nAChRb2(E61C)-IRES-GFP

Plasmid

#164777

Purpose

cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

Depositor

Alexandre Mourot

Article

Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

Insert

nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E61C

Promoter

pGK

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Il6

Plasmid

#207126

Purpose

Luciferase vector containing 3'UTR for mouse Il6

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Interleukin 6 (Il6) (Il6 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP.mIKKβ

Plasmid

#74412

Purpose

Expresses mouse eGFP-IKKβ in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

mouse IKKβ (Ikbkb Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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mFGF8a

Plasmid

#22088

Depositor

Gail Martin

Article

Crossley et al Development. 1995 Feb . 121(2):439-51.

Insert

FGF8 (Fgf8 Mouse)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pET28b_RanWT

Plasmid

#186270

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Use

Tags

His tagged

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-GFP

Plasmid

#106948

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pB-MultiplexedReporter-MOR170-1

Plasmid

#129461

Purpose

piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

MOR170-1 (Olfr895 Mouse)

Use

Synthetic Biology

Tags

LUCY tag, Rho tag

Expression

Mammalian

Mutation

Promoter

TRE (Tet-On)

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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3ME6/mSprouty2

Plasmid

#22097

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 2 (Spry2 Mouse)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
2.1 kB VEGF-luc

Plasmid

#27988

Depositor

Patricia D'Amore

Article

Loureiro et al Biochem Biophys Res Commun. 2005 Jan 14.

Insert

2.1 kB VEGF

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRL c-Myc 3'UTR

Plasmid

#14806

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

c-Myc 3'UTR (Myc Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pLNCX2-OptoTGFBRs

Plasmid

#118965

Purpose

Retroviral expression OptoTGFBRs for transient or stable constitutive expression

Depositor

Won Do Heo

Article

Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

Insert

OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)

Use

Retroviral

Tags

tdTomato

Expression

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5 MEKK4alpha WT

Plasmid

#12187

Depositor

Gary Johnson

Article

Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

Insert

MEKK4 (Map3k4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
SBP-EGFP-Ecadherin_puromycin

Plasmid

#65292

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcdna 3.1 DSG-3 Tension Sensor

Plasmid

#182717

Purpose

expresses DSG-3 TSmod

Depositor

Daniel Conway

Article

Jin et al bioRxiv [Preprint]. 2024 May 6:2024.05.0

Insert

desmoglein-3 (Dsg3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

TSmod inserted between G726 and G727

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-HCN4 [N114/10R-2b]

Plasmid

#206653

Purpose

Mammalian Expression Plasmid of anti-HCN4 (Human). Derived from hybridoma N114/10-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-HCN4 (Homo sapiens) recombinant Mouse monoclonal antibody (HCN4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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HCN4 scFv [N114/10]

Plasmid

#190526

Purpose

Mammalian Expression of HCN4 scFV. Derived from hybridoma N114/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

HCN4 (Homo sapiens) recombinant scFV (HCN4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pKmyc-Borg3

Plasmid

#141074

Purpose

Expresses Borg3 (Cdc42ep5) in mammalian cells

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol. 2001 Oct;3(10):861-6. doi

Insert

Borg3 (Cdc42ep5 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Zeb2-3'UTR

Plasmid

#61771

Purpose

Luciferase reporter containing the 3'UTR of mouse Zeb2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zeb2 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pMK285 (Stag-3FLAG-Bsr)

Plasmid

#72801

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Bsr

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #2

Plasmid

#61514

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gccgctcccggatctcctgc

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-Nkx6.1

Plasmid

#45846

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Nkx6.1 (Nkx6-1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tet-ON

Available since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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mLama1 AAV sgRNA 1, 2, 5

Plasmid

#135339

Purpose

Expression plasmid of VP64-SadCas9 sgRNA 1, 2 and 3

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

Lama1 VP64-dCas9 sgRNA Guides

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DNFGFR1-HA

Plasmid

#130887

Purpose

Expresses dominant negative mutant mouse FGFR1

Depositor

Gian-Paolo Dotto

Article

Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

Insert

Fgfr1 (Fgfr1 Mouse)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Dominant Negative receptor lacking cytosolic cata…

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd

Plasmid

#135518

Purpose

Mammalian expression of myc-tagged H2-Dd

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

C121S

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-GFP [N86/38R-1]

Plasmid

#206719

Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/38-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant Mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV500 A-ATF2

Plasmid

#33362

Depositor

Charles Vinson

Insert

ATF

Use

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see comments)

Promoter

CMV

Available since

Dec. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mFzd4

Plasmid

#42256

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 4 (Fzd4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
Flag-Gsdmd-NT C77A

Plasmid

#133889

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT-PDGFRa-IRES-Katushka

Plasmid

#133081

Purpose

Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Insert

PDGFRa (Pdgfra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Munc18cwt-pDEST27

Plasmid

#85626

Purpose

GST tagged Munc18c

Depositor

David James

Article

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
NRCoC

Plasmid

#74300

Purpose

Mammalian expression of full-length treacle fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-FKBP-STING(pLxIS)

Plasmid

#221333

Purpose

Overexpression of STING CTT in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Tags

6xHis-4xFKBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mRuby-LC-Myosin-C-10

Plasmid

#55870

Purpose

Localization: Light Chain Myosin, Excitation: 558, Emission: 605

Depositor

Michael Davidson

Insert

LC-Myosin

Use

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1

Plasmid

#124651

Purpose

Soma-localized ChrimsonR for dense expression in excitatory neurons

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Has Service

AAV9

Insert

ChrimsonR

Use

AAV

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CamKIIa

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Ntn4-AP-His

Plasmid

#71980

Purpose

Expresses the entire Netrin 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn4 (Ntn4 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-hCD2-CD14 (4R)

Plasmid

#133062

Purpose

Retroviral expression of mouse CD14 mutant "4R" deficient in LPS binding

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

CD14 (4R) (Cd14 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

CD14 allele defective for LPS binding (mutations …

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA2628_pGL4-UBCmin-luc2/Tbx3-B'

Plasmid

#124201

Purpose

Luciferase reporter driven by the Tbx3 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Tbx3 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-Sox13

Plasmid

#200194

Purpose

Expression of full length Sox13

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-Sox13 (Sox13 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-iRFP670

Plasmid

#197202

Purpose

Expresses the protein of iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP670

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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