4,074 results

1. pCRY2FL(deltaNLS)-mCherryN1

   Plasmid

   #26871

   Purpose

   Mammalian expression of cytoplasmic CRY2-mCherry

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   NLS sequences deleted

   Promoter

   CMV

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmStrawberry-Atg4BC74A

   Plasmid

   #21076

   Depositor

   Tamotsu Yoshimori

   Article

   Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

   Insert

   Atg4B(C74A) (Atg4b Mouse)
   

   Use

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   220-222 TGC(Cys) is changed to GCA (Ala)

   Promoter

   Available since

   June 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. TtRMPVIR

   Plasmid

   #27995

   Depositor

   Scott Lowe

   Article

   Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

   Insert

   TtRMPVIR (shRen)
   

   Use

   RNAi and Retroviral; Fluorescent reporters

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-CaMPARI W391F V398L

   Plasmid

   #60787

   Purpose

   Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI W391F V398L
   

   Use

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNANOG-Luc

   Plasmid

   #25900

   Depositor

   Ren-he Xu

   Article

   Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

   Insert

   NANOG promoter (NANOG Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBabe 12S E1A

   Plasmid

   #18742

   Depositor

   Scott Lowe

   Article

   Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

   Insert

   12S E1A
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDD284

   Plasmid

   #66825

   Purpose

   TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   TagRFP-T-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized TagRFP-T

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

8. MigR1-AE9a

   Plasmid

   #12433

   Depositor

   Dong-Er Zhang

   Article

   Yan et al Nat Med. 2006 Aug . 12(8):945-9.

   Insert

   AML1-ETO9a fusion (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   AML-ETO protein with ETO exon 9a.

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-hPIASy

   Plasmid

   #15208

   Depositor

   Ke Shuai

   Article

   Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

   Insert

   PIASy (PIAS4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 HA Arf6 ActQ67L

    Plasmid

    #10835

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 Q67L (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q67L. Defective in GTP hydrolysis. Constitutive…

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSQT834

    Plasmid

    #53371

    Purpose

    Csy4 and Cas9 nuclease expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 deltaN47 Beta-catenin

    Plasmid

    #19287

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-47

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-4T1 SHP2 WT

    Plasmid

    #8322

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV6M-Pak1 K299R

    Plasmid

    #12210

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Catalytically inactive Pak1: K299R

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4-Beclin1-HA

    Plasmid

    #24399

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTRE-TIGHT-EGFP-donor fw copy

    Plasmid

    #22074

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    TETO-eGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-HA-14-3-3 epsilon

    Plasmid

    #13273

    Depositor

    Michael Yaffe

    Insert

    14-3-3 epsilon (YWHAE Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pHCMV-LCMV-WE

    Plasmid

    #15793

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    Lymphocytic choriomeningitis virus glycoprotein LCMVGP gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV H-Ras V12 IRES GFP

    Plasmid

    #18780

    Depositor

    Scott Lowe

    Insert

    H-Ras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    V12

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMK291 (mAID-mClover-Bsr)

    Plasmid

    #72829

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Bsr
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMSP1E1

    Plasmid

    #20062

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E1 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeat of hel…

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHA-PUMA

    Plasmid

    #16588

    Depositor

    Bert Vogelstein

    Article

    Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

    Insert

    PUMA (BBC3 Human)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-300(HAT-)

    Plasmid

    #23254

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H1415A E1423A Y1424A L1428S Y1430A H1434A

    Promoter

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-f:PGC1b

    Plasmid

    #1031

    Depositor

    Bruce Spiegelman

    Article

    Lin et al J Biol Chem 2002 Jan 18;277(3):1645-8. E

    Insert

    PGC-1 beta (Ppargc1b Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pIREShyg-PA-GFP-alpha Tubulin

    Plasmid

    #12296

    Depositor

    Patricia Wadsworth

    Article

    Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA cyclin D1 HA

    Plasmid

    #11181

    Depositor

    Bruce Zetter

    Article

    Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

    Insert

    cyclin D1 (CCND1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. -1748 human cyclin D1 promoter pGL3Basic

    Plasmid

    #32726

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase; Reporter gene

    Tags

    Expression

    Mutation

    Promoter

    -1748CCND1 promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO.1 puro GFP siRNA

    Plasmid

    #12273

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    GFP siRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PKC delta DN

    Plasmid

    #16389

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC delta isoform 2 (Prkcd Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative (kinase inactive): K376R

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA4-TO-Puromycin-mVenus-MAP

    Plasmid

    #44118

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Use

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MDH1-PGK-GFP_2.0

    Plasmid

    #11375

    Purpose

    Retroviral construct for miRNA expression

    Depositor

    Chang-Zheng Chen

    Article

    Chen et al Science. 2004 Jan 2. 303(5654):83-6.

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFP-PCNA-IRES-puro2b

    Plasmid

    #26461

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    pEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pWZL-Blast-Flag-HA-hTERT

    Plasmid

    #22396

    Depositor

    William Hahn

    Article

    Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

    Insert

    TERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCVL SFFV d14GFP Donor

    Plasmid

    #31475

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d14GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted 14 amino acids on the c terminus of eGFP

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PGKneotpAlox2

    Plasmid

    #13444

    Depositor

    Philippe Soriano

    Article

    Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

    Insert

    PGKneotpAlox2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1/opto-b2AR-EYFP

    Plasmid

    #20948

    Purpose

    Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Mar 18. ():.

    Insert

    opto-b2AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pY001 (pFnCpf1_full)

    Plasmid

    #69973

    Purpose

    Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-double floxed-eNpHR-EYFP-WPRE-pA

    Plasmid

    #20949

    Purpose

    Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    halorhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 809 pcDNA3 GFP PTEN

    Plasmid

    #10759

    Depositor

    William Sellers

    Article

    Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. OCT4-eGFP-2A-Puro

    Plasmid

    #31939

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Inserts

    OCT4 (POU5F1 Human)
    eGFP (eGFP )
    

    Use

    Targeting donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge