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Showing: 3761 - 3800 of 158520 results

  1. pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

    Plasmid
    #203349
    Purpose
    Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    mGreenLantern
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203349-plasmid-map-sequence-id-399147

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  12. CIRTS-control

    Plasmid
    #213788
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:
    Insert
    CIRTS-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    213788-plasmid-map-sequence-id-424349

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  18. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

    Plasmid
    #178320
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi
    Inserts
    HRE RedFirefly reporter
    P53 FLuc reporter
    AP-1 Renilla reporter
    FOXO NLuc Reporter
    SMAD GrRenilla reporter
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178320-plasmid-map-sequence-id-363290

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  20. pJYS1Ptac

    Plasmid
    #85545
    Purpose
    Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter
    Depositor
    Sheng Yang
    Article
    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.
    Inserts
    Cpf1
    recT
    Use
    CRISPR; Shuttle vector corynebacterium glutamicum…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac and unknown
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85545-plasmid-map-sequence-id-176966

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  21. pMSCV-IRES-mAmetrine 1.1 FP

    Plasmid
    #52113
    Purpose
    a bicistronic IRES-mAtetrine containing retroviral vector
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES-mAmetrine 1.1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    52113-plasmid-map-sequence-id-80499

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  23. pBAD-6xHis-AviTag-ALFAtag-eGFP

    Plasmid
    #195854
    Purpose
    Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    6xHis-AviTag-ALFAtag-eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195854-plasmid-map-sequence-id-383965

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  26. pCS2 GFP-GSK3mut-MAPK

    Plasmid
    #29690
    Purpose
    GFP reporter protein fused to three mutated GSK3 phosphorylation sites
    Depositor
    Edward De Robertis
    Article
    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.
    Insert
    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
    Use
    Tags
    Flag and Flag-EGFP
    Expression
    Mammalian
    Mutation
    recognition sites only, and E3 ligase site, GSK3 …
    Promoter
    SP6
    Available since
    Jan. 6, 2012
    Availability
    Academic Institutions and Nonprofits only
    29690-plasmid-map-sequence-id-129831

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  30. IDG_TMC3_OE_1

    Plasmid
    #161629
    Purpose
    Overexpresses TMC3
    Depositor
    Mike McManus
    Insert
    TMC3 (TMC3 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    161629-plasmid-map-sequence-id-319361

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  33. FokI-dCas9

    Plasmid
    #52970
    Purpose
    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
    Depositor
    David Liu
    Article
    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    Fok1 fused to dCas9
    Use
    CRISPR
    Tags
    Fok1 nuclease (homodimeric wildtype) and NLS
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9
    Promoter
    CMV
    Available since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    52970-plasmid-map-sequence-id-82408

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  38. pCDH-EF1-copGFP-T2A-Puro

    Plasmid
    #72263
    Purpose
    Express copGFP and puromycin resistance gene under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72263-plasmid-map-sequence-id-134557

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Showing: 3761 - 3800 of 158520 results