159,461 results

1. pEGFP_mNG2(11)_Clathrin light chain

   Plasmid

   #82608

   Purpose

   Expresses mNG2(11) tagged clathrin light chain in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   mNG2(11)_Clathrin light chain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. JunB

   Plasmid

   #187903

   Purpose

   Expresses JunB in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   JunB (Junb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-CD14

   Plasmid

   #13645

   Depositor

   Doug Golenbock

   Insert

   CD14 (CD14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-mito-mTagBFP2

   Plasmid

   #216130

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-CAG-GFP

   Plasmid

   #28014

   Depositor

   Karel Svoboda

   Article

   Mao et al Neuron. 2011 Oct 6;72(1):111-23.

   Insert

   eGFP
   

   Use

   AAV; Adeno associate viral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX304_CMV::mCherry-LgBiT-FLAG-NES

   Plasmid

   #199715

   Purpose

   Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   mCherry-LgBiT-FLAG-NES
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-C1 R62D actin-3XNLS P2A mCherry

   Plasmid

   #58477

   Purpose

   Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Inserts

   beta actin (ACTB Human)
   3XNLS
   P2A cleavage site
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed Arginine 62 to Aspartic Acid

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-sgR26

   Plasmid

   #127376

   Purpose

   Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-Vimentin-7

   Plasmid

   #56439

   Purpose

   Localization: Intermediate Filaments, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAAV-hSyn-GRAB-rDA3m

    Plasmid

    #208702

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. NLS-mCherry-LEXY (pDN122)

    Plasmid

    #72655

    Purpose

    Blue light-inducible nuclear export of mCherry

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Insert

    NLS-mCherry-LEXY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Use

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gal4-VP16

    Plasmid

    #71728

    Purpose

    plasmid expressing fusion construct of Gal4 DBD and VP16 AD

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    Gal4 DBD(1-147) (GAL4 Budding Yeast)
    

    Use

    Tags

    VP16 AD (413-490)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. 2xP53_RE::FLuc

    Plasmid

    #178316

    Purpose

    P53 Firefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    P53 FLuc Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p23-puro-EGFP-RPA194

    Plasmid

    #179237

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    RPA194 (POLR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCH45 (multiAsCas12a-KRAB lenti)

    Plasmid

    #217330

    Purpose

    Lentiviral expression of multiAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    UCOE-SFFV

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBiFC-VN173

    Plasmid

    #22010

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VN173
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Venus residus 1-172

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV.CBA.eGFP.2A.P301L-Tau

    Plasmid

    #140425

    Purpose

    AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau(P301L) (MAPT Synthetic, Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mutation

    changed Proline 301 to Leucine

    Promoter

    CBA

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-TetOne-Puro-hAXL

    Plasmid

    #124797

    Purpose

    Tet-inducible AXL expression in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p006-GFP-strong

    Plasmid

    #108313

    Purpose

    GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFAB4026

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hBax C3-EGFP

    Plasmid

    #19741

    Depositor

    Richard Youle

    Article

    Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

    Insert

    Bax (BAX Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCherry-LaminA-C-18

    Plasmid

    #55068

    Purpose

    Localization: Nuclear Envelope, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNLS-iRFP670

    Plasmid

    #45466

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FURIN-bio-His

    Plasmid

    #51755

    Purpose

    Expresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    FURIN (FURIN Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. HA-hULK1

    Plasmid

    #31963

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK1 (ULK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 4XCLEAR-luciferase reporter

    Plasmid

    #66800

    Purpose

    Reporter for activity of the CLEAR network

    Depositor

    Albert La Spada

    Article

    Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

    Insert

    4XCLEAR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Four CLEAR elements (GTCACGTGAC) in tandem derive…

    Available since

    Nov. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRK5-HA-Ubiquitin-K48R

    Plasmid

    #17604

    Purpose

    Mammalian expression of HA tagged ubiquitin K48R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48R (expected to disrupt G76-K48 ubiquitin chain…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJC175e

    Plasmid

    #79219

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PPSP gIII, luxAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPSP

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX6P1-mCherry-Nanobody (LaM-2)

    Plasmid

    #162276

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    mCherry-Nanobody (LaM-2)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AICSDP-8:TOMM20-mEGFP

    Plasmid

    #87423

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. Bxb1-GA_CAG-EGFP

    Plasmid

    #229799

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Olig001

    Plasmid

    #170716

    Purpose

    Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

    Depositor

    Thomas McCown

    Article

    Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

    Insert

    Olig001 capsid gene / rep 2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRK793

    Plasmid

    #8827

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219V mutant
    

    Use

    Tags

    His, MBP (with TEV), and polyarginine

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability)

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-Mito-ExRai-AMPKAR

    Plasmid

    #192448

    Purpose

    ExRai-AMPKAR targeted to the mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Mito-ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GW1-PercevalHR

    Plasmid

    #49082

    Purpose

    Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-Popt

    Plasmid

    #231705

    Purpose

    Mammalian expression vector encoding a codon-optimized P gene from vesicular stomatitis virus, strain Indiana.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV P gene (codon-optimized )
    

    Use

    Vsv expression

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-Lopt

    Plasmid

    #231706

    Purpose

    Mammalian expression vector encoding a codon-optimized L gene from vesicular stomatitis virus, strain Indiana.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV L gene (codon-optimized )
    

    Use

    Vsv expression

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pnCS_SEPT7_SEPT9_i1-TEV-Strep

    Plasmid

    #174500

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-CAG-tdTomato (codon diversified)

    Plasmid

    #59462

    Purpose

    AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter

    Depositor

    Edward Boyden

    Has Service

    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    codon diversified

    Promoter

    CAG

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CROPseq-multi-Puro (pRW1441)

    Plasmid

    #216217

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge