8,687 results

1. pCIBN(deltaNLS)-pmGFP

   Plasmid

   #26867

   Purpose

   Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   NLS sequences mutated

   Promoter

   CMV

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1+ hnRNPK myc HIS

   Plasmid

   #71662

   Purpose

   Mammalian expression of hnRNPK

   Depositor

   Gideon Dreyfuss

   Insert

   hnRNPK (HNRNPK Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCitrine-N1

   Plasmid

   #54594

   Purpose

   Localization: N1 Cloning Vector, Excitation: 516, Emission: 529

   Depositor

   Michael Davidson, Oliver Griesbeck, Robert Campbell, Roger Tsien

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Type

   Empty backbone

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pLifeAct-HyPer7

   Plasmid

   #136464

   Purpose

   Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FU-tetO-Gateway

   Plasmid

   #43914

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-FLAG-MTOR-S2215Y

   Plasmid

   #69013

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S2215Y

   Promoter

   CMV

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-Flag-hMcl-1

   Plasmid

   #25392

   Depositor

   Roger Davis

   Insert

   MCL1 (MCL1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MaLionG

   Plasmid

   #113906

   Purpose

   Green ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   MaLionG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-Myc-14-3-3ζ

   Plasmid

   #48798

   Purpose

   expresses human myc-14-3-3ζ in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Chen et al Cell. 2003 May 16;113(4):457-68.

   Insert

   YWHAZ (YWHAZ Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSET-6xTR-TUBE

    Plasmid

    #110313

    Purpose

    E. coli expression and purification of 6xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    6xTR-TUBE (UBQLN1 Human)
    

    Use

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAR-Ec633

    Plasmid

    #130873

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L477V, L640Y, I777K, W814N

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLJM1-FLAG-GFP-Tmem192

    Plasmid

    #134630

    Purpose

    lysosomal marker

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFlag-CMV1-hMD2

    Plasmid

    #13028

    Depositor

    Doug Golenbock

    Insert

    MD2 (LY96 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PresentER-SIINFEKL (GFP)

    Plasmid

    #102944

    Purpose

    PresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Insert

    SIINFEKL (OVAL Chicken, Synthetic)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    OVA257-264

    Promoter

    LTR

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBiFC-VN155(I152L)

    Plasmid

    #27097

    Depositor

    Chang-Deng Hu

    Article

    Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

    Insert

    VN155(I152L)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    I152L

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCardinal-pBAD

    Plasmid

    #54800

    Purpose

    Localization: Protein Expression Vector , Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    6xHis and mCardinal

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pCIS GEM-CEPIA1er

    Plasmid

    #58217

    Purpose

    Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    GEM-CEPIA1er
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GEM-GECO1 E298D F359W D400E E407D

    Promoter

    CAG

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-EglN3-pcDNA3

    Plasmid

    #18960

    Depositor

    William Kaelin

    Article

    Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

    Insert

    Egg laying Nine - 3 (EGLN3 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

    Plasmid

    #111568

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Has Service

    AAV5

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-CD68 promoter/enhancer

    Plasmid

    #34837

    Depositor

    Peter Murray

    Article

    Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

    Insert

    CD68 promoter/enhancer -680/+140 (CD68 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 3XMEF2-luc

    Plasmid

    #32967

    Purpose

    Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

    Depositor

    Ron Prywes

    Insert

    MEF2 binding sites (MEF2A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    MEF2

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-FLAG-Rheb

    Plasmid

    #19996

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 (RHEB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

    Plasmid

    #31482

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce Target) EF Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry has M9S and M16L mutations. This is what …

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR_PGK

    Plasmid

    #79120

    Purpose

    Lentiviral vector for constitutive expression of genes (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYPQ150

    Plasmid

    #69301

    Purpose

    Gateway entry vector with pcoCas9 (Plant codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pcoCas9 (plant codon-optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-I-PhoCl-HCVp

    Plasmid

    #87697

    Purpose

    Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
    

    Use

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUltra-hot

    Plasmid

    #24130

    Purpose

    3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest

    Depositor

    Malcolm Moore

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2-HA-GAAZFP-FokI-RR

    Plasmid

    #18754

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    zinc finger nuclease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    RNA expression vector for expressing the zinc fin…

    Promoter

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-SIRT1 H363Y

    Plasmid

    #1792

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H363Y, deacetylase domain mutation

    Promoter

    Available since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-FLAG-Rheb-N153T

    Plasmid

    #19997

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 - N153T (RHEB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Asparagine 153 to Threonine

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

32. pMK347 (BSD-P2A-mAID)

    Plasmid

    #121181

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    BSD-P2A-mAID
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pR26 CAG/GFP Dest

    Plasmid

    #74281

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP-C1-MKLP1

    Plasmid

    #70145

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-Myd88-CFP

    Plasmid

    #13026

    Depositor

    Doug Golenbock

    Insert

    myeloid differentiation primary response gene (88) (MYD88 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. eIF2a 1

    Plasmid

    #21807

    Depositor

    David Ron

    Insert

    eIF2a (EIF2S1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pXPR_047

    Plasmid

    #107145

    Purpose

    measures Cas9 activity

    Depositor

    David Root

    Insert

    GFP + sgRNA for GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MICU-APEX2

    Plasmid

    #79057

    Purpose

    A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    C-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV immearly promotor

    Available since

    Sept. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GFP-LKB1

    Plasmid

    #21147

    Depositor

    Junying Yuan

    Article

    Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

    Insert

    GFP-LKB1 (Stk11 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only