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pcDNA3.3_LIN28A
Plasmid#26819DepositorInsert5'UTR-LIN28A-3'UTR (LIN28A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR SFFVp Phy-mCherry-CAAX
Plasmid#50839PurposeOptogenetic PI3K activation system; membrane componentDepositorInsertPhyB 1-908
UseLentiviral and Synthetic BiologyTagsmCherryExpressionMutationPromoterSFFVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-GFP11-Clathrin light chain
Plasmid#70217PurposeExpresses GFP11-clathrin light chain in mammalian cellsDepositorInsertGFP11-Clathrin light chain (CLTA Human)
UseTagsGFP11ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-Ftractin-mCherry
Plasmid#85131Purposelentiviral expression of an F-actin live-cell marker (mCherry)DepositorInsertFtractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
UseLentiviralTagsmCherryExpressionMutationPromoterEF1aAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC13N-dCas9-BFP-KRAB
Plasmid#127968Purposeconstitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)DepositorInsertCLYBL-CAG-dCas9-NLS-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Atg7
Plasmid#24921DepositorInsertAtg7 (ATG7 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR-PP7-2xmCherry-CAAX
Plasmid#74925PurposeExpresses PP7-2xmCherry-CAAXDepositorInsertPP7-2xmCherry-CAAX
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNS35 (multiAsCas12a piggyBac)
Plasmid#217336PurposepiggyBac expression of multiAsCas12aDepositorInsertmultiAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-P2A-TagBFP2ExpressionMammalianMutationR1226A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiSAM v2 (Puro)
Plasmid#92062PurposeModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 (sgRNA) and EF1a (dCas9-VP64)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT1
Plasmid#36939PurposeMammalian expression of human DNMT1 with myc tagDepositorInsertDNMT1 (DNMT1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6-210
Plasmid#86919PurposeFluorescent reporter for ER calcium signalingDepositorInsertER-GCaMP6-210
UseLentiviralTagsExpressionMammalianMutationK78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…PromoterCAMKIIAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330S-2
Plasmid#58778PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKII-TRPV1-P2A-mCherry
Plasmid#200829PurposeTransduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporterDepositorInsertTRPV1-P2A-mCherry (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCAC-69
Plasmid#31241PurposeMitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafishDepositorInsertmitochondrial localization enhanced green fluorescent protein
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFAM-SNAPf
Plasmid#187909PurposeMammalian cell expression of SNAPf-labeled TFAMDepositorInsertTFAM (TFAM Human)
UseTagsSNAPfExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cFos-XRI-V5
Plasmid#178058PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.DepositorInsertXRI-V5
UseAAVTagsV5-MBPExpressionMammalianMutationPromoterMouse c-fos promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRXRα
Plasmid#135910PurposeHuman Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 PromoterDepositorInsertHuman retinoid X receptor- alpha (RXRA Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-3FLAG-EEA1
Plasmid#176491PurposeFor lentiviral transduction of 3FLAG-EEA1DepositorInsertEEA1 (EEA1 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRCaMP1a
Plasmid#61562PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRCaMP1a
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G
Plasmid#177960PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)DepositorInsertSARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
UseTagsExpressionMammalianMutationD614GPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN-EphA2-Flag
Plasmid#102755PurposeExpression of EphA2DepositorInsertEphA2 (EPHA2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-M13-TEV-C-P2A-TdTomato
Plasmid#92391Purposeproduces AAV expressing Cal-light M13-TevC component and TdTomatoDepositorInsertM13-TEV-C-P2A-TdTomato
UseAAV and Synthetic BiologyTagsTdTomatoExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
mApple-Alpha-V-Integrin-25
Plasmid#54866PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592DepositorInsertAlpha-V Integrin (ITGAV Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_S5E2_Chrimson_tdTomato
Plasmid#219432PurposeAAV construct expressing Chrimson-tdTomato under the control of E2 regulatory elementDepositorInsertChrimson-tdTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBR-Cas9ΔyqhD-HA500
Plasmid#220369PurposeA single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440DepositorInsertsSpCas9
sgRNA for yqhD in P. putida KT2440
UseCRISPR; Broad host rangeTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-NLS-HA
Plasmid#141321PurposePlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)DepositorInsertRfx-Cas13d (NLS-RfxCas13d-NLS)
UseCRISPRTagsHA and NLSExpressionMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-ultraID-Blast
Plasmid#207715PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a Halotag-CNA35
Plasmid#131128PurposeHalotag-CNA35 peptide for Collagen labellingDepositorArticleInsertHalotag-CNA35
UseTagsHalotagExpressionBacterialMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-UbC-Blast-2A-STING-mNeonGreen
Plasmid#227185PurposeLentiviral expression plasmid encoding STING-mNeonGreen under UbC promoterDepositorInsertSTING1 (STING1 Human)
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterUbCAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXN21-Dest
Plasmid#105086PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneUseTagsNubGExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-FLAG-CAMK2D
Plasmid#221699PurposeLentiviral expression of FLAG-tagged human CAMK2D in mammalian cellsDepositorInsertCAMK2D (CAMK2D Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shGFP
Plasmid#110318PurposeControl (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2DepositorInsertGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EGFP_Donor
Plasmid#178088PurposeHDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV SPOP 1-374
Plasmid#197015PurposeE. coli expression vectorDepositorInsertSPOP (Spop Mouse)
UseTags6His-SUMO-TEVExpressionBacterialMutationPromoterAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc hygro
Plasmid#67502PurposeConstitutive expression of firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe_Hyg_cOVA_T2A_mStrawberry
Plasmid#161737PurposeExpression of cOVA and mStrawberryDepositorInsertcytoplasmic ovalbumin
UseRetroviralTagsmStrawberryExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon 300
Plasmid#163115PurposeRed fluorescent protein-based glucose sensor for mammalian cells with higer affinityDepositorInsertRed Glifon 300
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.2.t1
Plasmid#73546PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.2.t1
pAzFRS.2.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.NRAS
Plasmid#83173PurposeGateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions)DepositorInsertNRAS (NRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only
