166,201 results

1. pLJC2-Blast-DHFR

   Plasmid

   #233893

   Purpose

   lentiviral overexpression vector for DHFR

   Depositor

   Jason Cantor

   Article

   Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

   Insert

   DHFR (DHFR Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. BLA_CAT_K136_GPG_LOV2

   Plasmid

   #231575

   Purpose

   This plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue light

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   CAT_ carrying an AsLOV2 insertion behind K136
   

   Expression

   Bacterial

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IP-EGFP-PA-BAX(lyso)

   Plasmid

   #228939

   Purpose

   Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   TMEM106B (TMEM106B Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus and C-terminus are deleted. BAX(3-171)…

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG_GST-TEV-WIPI3

   Plasmid

   #223800

   Purpose

   Expression of recombinant protein for purification

   Depositor

   James Hurley, Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   WIPI3 (WDR45B Human)
   

   Tags

   GST-TEV

   Expression

   Mammalian

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXJ-Myc-IRF8

   Plasmid

   #232668

   Purpose

   transient expresses IRF8 in mammalian cells

   Depositor

   Chen-Yang Shen

   Insert

   IRF8 (IRF8 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0442

   Plasmid

   #143026

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZFX (ZFX Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBeta-MHC-promoter DV (JDW 26)

   Plasmid

   #229835

   Purpose

   A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP13592 - pAAV-AiE0519h_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3592)

   Plasmid

   #229914

   Purpose

   AiE0519h_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS416-HIS3p-TID-FKBP-HA3

   Plasmid

   #228006

   Purpose

   Expresses TurboID-FKBP fusion from HIS3 promoter; yeast BioID

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID-FKBP
   

   Tags

   HA tag

   Expression

   Yeast

   Promoter

   HIS3

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFa6-TID-FKBP-3HA-KanMX6

    Plasmid

    #228024

    Purpose

    To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICOZ-MAG[EF1α-HER1 CD28Z]

    Plasmid

    #218335

    Purpose

    Donor plasmid of MAG Transposon with HER1 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    HER1 CAR mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-[EF1α-HER1 CD28Z]

    Plasmid

    #218339

    Purpose

    Lentiviral transfer plasmids for mammalian expression HER1 CAR

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    HER1 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRLSIN-[EF1α-HER1 4-1BB]

    Plasmid

    #218341

    Purpose

    Lentiviral transfer plasmids for mammalian expression HER1 CAR

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    HER1 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH2.156_LV-Zeo

    Plasmid

    #205554

    Purpose

    Express zeocin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Zeo
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF1897

    Plasmid

    #142285

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR2C1 (NR2C1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDx_mScarlet-SYFP2

    Plasmid

    #189762

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB80-hsKIF5B(1-353)GCN4-L-GFP

    Plasmid

    #193714

    Purpose

    Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Human, Synthetic)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-353);

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB80-hsKIF5B(1-353)-GCN4-L-mCherry

    Plasmid

    #193715

    Purpose

    Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Human, Synthetic)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-353); mCherry: Met1 Deleted

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

    Plasmid

    #193719

    Purpose

    Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
    

    Tags

    2xHA-FRB

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

    Plasmid

    #193721

    Purpose

    Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Human, Synthetic)
    

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCAG-MBP-ATG9 (692-839)

    Plasmid

    #188092

    Purpose

    To make monomeric ATG9 C-terminal tail for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (692-839)

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pETM33_YES1_SH3

    Plasmid

    #178492

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    YES1_SH3 (YES1 Human, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid

    #177208

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pDY153: Flag(SUZ12) WT HDR plasmid

    Plasmid

    #170793

    Purpose

    WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

    Depositor

    Thomas Cech

    Article

    Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

    Insert

    SUZ12 (SUZ12 Human)
    

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

    Plasmid

    #170792

    Purpose

    This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

    Depositor

    Thomas Cech

    Article

    Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

    Insert

    Cas9
    

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3.1(+) eGFP SH3BP5 G4

    Plasmid

    #129022

    Purpose

    Expresses an eGFP mRNA with the SH3BP5 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pHR-scFv-GCN4-mStayGold-GB1-dWPRE

    Plasmid

    #231598

    Purpose

    Express scFv_GCN4-mStayGold for SunTag translation imaging

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    scFv-GCN4-mStayGold
    

    Use

    Lentiviral

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. p28-2iDMD-puro

    Plasmid

    #88895

    Purpose

    28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.

    Depositor

    Michele Calos

    Article

    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

    Inserts

    Dystrophin (DMD Human)
    luciferase
    puromycin resistance enzyme
    mCherry
    

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225697

    Purpose

    Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (WT) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

    Plasmid

    #69901

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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31. pLVX-Puro-TDP-43-WT

    Plasmid

    #133753

    Purpose

    Wild type human TDP-43 in pLVX-Puro lentiviral vector

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PGK EGFP

    Plasmid

    #216110

    Purpose

    PGK EGFP

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    PGK promoter
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCXLE-EGFP

    Plasmid

    #27082

    Purpose

    Integration-free (episomal) expression of EGFP

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    eGFP
    

    Expression

    Mammalian

    Available Since

    March 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PLKO.1-Scrambled

    Plasmid

    #136035

    Purpose

    Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    None (Scrambled)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLVX_DSB_Spectrum-V2

    Plasmid

    #192475

    Purpose

    Lentiviral DSB Spectrum V2 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CROPseq-Guide-Puro-mCherry2

    Plasmid

    #219679

    Purpose

    CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

    Depositor

    Christoph Bock

    Article

    Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GZnP2 in pcDNA3.1+

    Plasmid

    #161739

    Purpose

    Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

    Depositor

    Yan Qin

    Article

    Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

    Insert

    GZnP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV4-ApoE3

    Plasmid

    #87086

    Purpose

    Expresses ApoE3 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E3 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET-Sac-Abeta(M1-40)

    Plasmid

    #71876

    Purpose

    Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-40) (APP Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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