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Showing: 5051 - 5100 of 6939 results

  6. pENTR1A mKate2-hu_ncOGT D554N

    Plasmid
    #154283
    Purpose
    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Gateway entry vector
    Tags
    2x FLAG and mKate2
    Expression
    Mutation
    D554N mutation (Ser/Thr Glycosylation Impaired)
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154283-plasmid-map-sequence-id-304858

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  21. pDO001

    Plasmid
    #198828
    Purpose
    Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::miniSOG-103L-VAMP2-mCherry::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198828-plasmid-map-sequence-id-390248

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  22. pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)

    Plasmid
    #199567
    Purpose
    Stable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transfer
    Depositor
    Yuxin Song, Andrea Cimarelli
    Article
    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    Trim69 isoform B (codon optimized) (TRIM69 Human)
    Use
    Retroviral; Allows for puromycin selection
    Tags
    Flag
    Expression
    Mutation
    codon optimized
    Promoter
    Available since
    May 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199567-plasmid-map-sequence-id-392223

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  31. pET24a-VHH-tev-std

    Plasmid
    #109423
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109423-plasmid-map-sequence-id-260701

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  34. pcDNA3-FLAG-LplA(AAG)

    Plasmid
    #61307
    Purpose
    Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression
    Depositor
    Alice Ting
    Article
    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11
    Insert
    E. coli lipoic acid ligase
    Use
    Tags
    6xHis, Flag, T7 tag, and Xpress epitope
    Expression
    Mammalian
    Mutation
    E20A, F147A, H149G
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61307-plasmid-map-sequence-id-99628

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  39. (p)odr-3::GFP::EGL-4

    Plasmid
    #100897
    Purpose
    GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.
    Depositor
    Noelle L'Etoile
    Article
    Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10
    Insert
    56-2741: odr-3 promoter
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    odr-3
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100897-plasmid-map-sequence-id-193490

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  47. PV225

    Plasmid
    #132334
    Purpose
    dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
    Depositor
    Joshua Young
    Article
    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.
    Insert
    dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
    Use
    Tags
    AT-CBF1
    Expression
    Plant
    Mutation
    Codon optimize for expression and stability in Ze…
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    132334-plasmid-map-sequence-id-258129

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  50. SunTagCACTA1g2-22aa-TET1cd

    Plasmid
    #106437
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    106437-plasmid-map-sequence-id-205837

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Showing: 5051 - 5100 of 6939 results