179,463 results
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Plasmid#201915PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-pol
ExpressionMammalianPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)
Plasmid#196703PurposePlasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.DepositorInserthuman RNaseH1 D210N
ExpressionMammalianMutationD210N, catalytically inactiveAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPRi(v2)-Blast
Plasmid#170068PurposeExpresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistanceDepositorInsertKRAB-dCas9-MeCP2
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianPromoterEFS-NSAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHage2-EF1a-TERT-IRES-Puro
Plasmid#182590Purposeexpresses TERT in mammalian cellsDepositorAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 -EGFP
Plasmid#165108Purposemammalian expression and localizationDepositorAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCh-Mfn2
Plasmid#141156PurposeMammalian expression and labeling mitofusin 2DepositorAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXG237
Plasmid#141281PurposeATF4 translational reporterDepositorInsertuORF1/2 of ATF4 followed by mApple
UseLentiviralTagsmApplePromoterCMVAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
8xNFAT-ZsG-hCD8
Plasmid#153417PurposeNFAT-driven ZsGreen-1 reporter gene with human CD8ab geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
UseRetroviralExpressionMammalianAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV Retrograde)
Viral prep#26975-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CamKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 TFAM-mScarlet
Plasmid#129573PurposeExpression of mScarlet fused TFAMDepositorAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-HA-NONO
Plasmid#127655PurposeOver-express HA-tagged human NONODepositorAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)
Plasmid#126580PurposeA repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locusDepositorInsertOsARF16-PB1 domain
TagsHAExpressionMammalianAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
Plasmid#122151PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.DepositorInsertdCas9 fused to SunTag(24x)
UseLentiviralTags2xNLS-P2A-BFP and NLSExpressionMammalianPromoterSFFVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-BRAF-V600E
Plasmid#116204PurposeLentiviral expression of BRAF V600EDepositorAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pm-iATPSnFR1.0
Plasmid#102548Purposemembrane displayed ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.0
ExpressionMammalianAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENN.AAV.CMVs.Pl.Cre.rBG (AAV8)
Viral prep#105537-AAV8PurposeReady-to-use AAV8 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (AAV1)
Viral prep#100838-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (#100838). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6M calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (AAV9)
Viral prep#100838-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (#100838). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-U6insert-EF1puro
Plasmid#104537PurposeA piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAsDepositorTypeEmpty backboneUseCRISPR, Mouse Targeting, and Synthetic Biology ; …ExpressionMammalianPromoterU6Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-DIO EYFP (AAV2)
Viral prep#27056-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorTagsEYFP (Cre-dependent)Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
LbCpf1-2NLS
Plasmid#102566Purposebacterial expression of LbCpf1-2NLSDepositorInsertLbCpf1
Tags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-EN1201
Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
ExpressionMammalianAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
Tags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human CRISPR lncRNA activation library (SAM) in lenti sgRNA(MS2)_zeo backbone
Pooled library#1000000106PurposeHuman CRISPR Synergistic Activation Mediator (SAM) pooled library for the transcriptional activation of lncRNAsDepositorAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-Cj-sgRNA
Plasmid#89753PurposeU6 promoter driven expression of Cj-SgRNA cloned with BsmbIDepositorInsertCj-SgRNA
UseSgrna expression under u6 promoterPromoterU6Available sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE
Plasmid#85452PurposeLentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.DepositorInsertU6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
UseCRISPR and LentiviralTagsNLSExpressionMammalianAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-Cas9-T2A-EGFP-ires-puro
Plasmid#78311PurposeExpresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.DepositorInsertWT SpCas9-T2A-EGFP-ires-puromycin resistance
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianPromoterCAGAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TrkB mEGFP
Plasmid#83952PurposeFRET donor for dynamically measuring TrkB activity in neuronsDepositorAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3 HA C9ORF72 op
Plasmid#74156PurposeExpress HA tagged optimized C9ORF72 in mammalian cellsDepositorAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC13N-iCAG.copGFP
Plasmid#66578PurposeCLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENsDepositorInsertcopGFP
ExpressionMammalianPromoterCAGAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9-NAT
Plasmid#64329PurposeCas9 expression cassette carried by a yeast single-copy episome plasmidDepositorInsertHuman-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
UseCRISPRExpressionYeastAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCBC-MT1T2
Plasmid#50593PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, gRNA scaffold, OsU3t plus, TaU3p, T2
UseCRISPR; Pcr templateExpressionPlantAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTagRFP-Membrane-1
Plasmid#57992PurposeLocalization: Plasma Membrane, Excitation: 555, Emission: 584DepositorTypeEmpty backboneTagsN-terminal 20 Aas MLS from Neuromodulin and mTagR…ExpressionMammalianAvailable sinceDec. 5, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
mCherry-Rab4a-7
Plasmid#55125PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Calreticulin-N-16
Plasmid#55006PurposeLocalization: Endoplasmic Reticulum, Excitation: 587, Emission: 610DepositorAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTagRFP-T-Lifeact-7
Plasmid#54586PurposeLocalization: Actin, Excitation: 555, Emission: 584DepositorInsertLifeact-mTagRFP-T
ExpressionMammalianAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
lentiCRISPR - EGFP sgRNA 2
Plasmid#51761PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 2
UseCRISPR and LentiviralExpressionMammalianPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human Lentiviral sgRNA Library - Control
Pooled library#51048PurposeSub-pool of Sabatini/Lander sgRNA library enriched for control targets.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE HA Chk2 WT
Plasmid#41901DepositorAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TroponinT-GCaMP5-Zeo
Plasmid#46027DepositorInsertGCaMP5-2A-Zeo
UseLentiviralExpressionMammalianPromoterTNNT2Available sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-MAP1B
Plasmid#44396DepositorAvailable sinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4.20-HIF1A prom
Plasmid#40173DepositorInsertHIF1A promoter fragment (HIF1A Human)
ExpressionMammalianAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LYN11-FRB-mcherry
Plasmid#38004DepositorPublicationAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pGCDNsam-Hnf4a-IRES-GFP
Plasmid#33006DepositorInserthepatic nuclear factor 4 alpha (Hnf4a Mouse)
UseRetroviralAvailable sinceMarch 6, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AnkyrinG-190-GFP
Plasmid#31059DepositorAvailable sinceFeb. 28, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVS1
Plasmid#33143DepositorPublicationInsertLacO (256 copies)
ExpressionYeastAvailable sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-HDAC4-FLAG
Plasmid#30485DepositorAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PLKO-mcherry-luc-puro-Renilla cDNA
Plasmid#29783DepositorPublicationInsertRenilla
UseLentiviralExpressionMammalianAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pF145 pAcGFP1 SOD1G93A
Plasmid#26406DepositorPublicationAvailable sinceNov. 8, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by