Skip to main content
Addgene
Showing: 5551 - 5600 of 6949 results

  10. psicheck-2-LPPRC

    Plasmid
    #48167
    Purpose
    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element
    Depositor
    Vamsi Mootha
    Article
    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106
    Insert
    LRPPRC leucine rich pentatricopeptide repeat containing (Lrpprc Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. GST-CHMP4A (205-222) E209A (pGEX2T(TEV))

    Plasmid
    #80640
    Purpose
    Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Changed Glutamate 209 to alanine
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80640-plasmid-map-sequence-id-161707

    Enlarge

  17. pcDNA3.BRWD3-V5H

    Plasmid
    #48624
    Purpose
    Expresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cells
    Depositor
    Aziz Sancar
    Article
    Ozturk et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    BRWD3 (BRWD3 Fly)
    Use
    Tags
    V5 and His tags
    Expression
    Mammalian
    Mutation
    no mutations, stop was removed to fuse V5His at C…
    Promoter
    CMV
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. pcDNA3-Lyso-InPGrp

    Plasmid
    #236002
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to lysosome surface.
    Depositor
    Jin Zhang
    Article
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Insert
    Lyso-InPGrp (Cyth3 Rat, Synthetic)
    Use
    Tags
    Cerulean, Full-length LAMP1, and cpVenus[E172]
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236002-plasmid-map-sequence-id-473795

    Enlarge

  24. pcDNA3-Endo-INPP5E

    Plasmid
    #236005
    Purpose
    Endosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.
    Depositor
    Jin Zhang
    Article
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Insert
    Endo-INPP5E (INPP5E Synthetic, Human)
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236005-plasmid-map-sequence-id-473813

    Enlarge

  29. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Article
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pgdh and Pxyl
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

    Enlarge

  33. Shim_AsnRS_419.110_SHI02794_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228373
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Asparagine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 419.110 (SHI02794); determination of S. himalayana AsnRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana AsnRS evm.model.fragScaff scaffold 419.110 (SHI02794)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228373-plasmid-map-sequence-id-456195

    Enlarge

  34. Shim_GlyRS_87.19_SHI04113_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228377
    Purpose
    Expresses N-term transit peptide from S. himalayana Glycine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 87.19 (SHI04113RA); determination of Sapria himalayana GlyRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana GlyRS evm.model.fragScaff scaffold 87.19 (SHI04113RA)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228377-plasmid-map-sequence-id-455855

    Enlarge

  35. Shim_ProRS_3278.3.7_SHI28108_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228379
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Proline aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA); determination of S. himalayana ProRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana ProRS evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228379-plasmid-map-sequence-id-455871

    Enlarge

  36. Athal_PheRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228370
    Purpose
    Expresses N-term transit peptide from Arabidopsis PheRS At3g58140 with demonstrated dual mito/plastid targeting; proof-of-concept for Plasmid 228371
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from Arabidopsis PheRS At3g58140 (Warren et al., 2023) with demonstrated dual mito/plastid targeting.
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228370-plasmid-map-sequence-id-455840

    Enlarge

Showing: 5551 - 5600 of 6949 results