We narrowed to 6,120 results for: nls
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Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNPK-NLS-Vika
Plasmid#79969Purposeexpresses human codon-optimized Vika-recombinase in mammalian cellsDepositorInserthuman codon-optimized Vika sequence, NLS tagged
UseTagsNLSExpressionMammalianMutationPromoterPGKAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
dCas9-AsLOV2x3-NLSx3
Plasmid#231566PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP
Plasmid#32628DepositorInsertSce
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-mCherry-NLS
Plasmid#194049PurposeTransient expression of mCherry-NLS in plant cell (Nucleus)DepositorInsertmCherry
UseTagsSV40 (Nuclear localization signal)ExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIBN(deltaNLS)-pmGFP
Plasmid#26867PurposeExpresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membraneDepositorInsertCIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
UseTagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
KillerRed-LacI-NLS
Plasmid#215102PurposeExpresses KillerRed-LacI-NLS in mammalian cells.DepositorInsertKillerRed-LacI-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRvTUB-mEGFP-NLS
Plasmid#205011PurposeThe vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-NLS-HA
Plasmid#141321PurposePlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)DepositorInsertRfx-Cas13d (NLS-RfxCas13d-NLS)
UseCRISPRTagsHA and NLSExpressionMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mEGFP-NLS
Plasmid#205005PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-mEGFP-NLS
Plasmid#205004PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GCamp6-3NLS
Plasmid#165401PurposeExpresses a nuclear localized genetically encoded calcium reporter.DepositorInsertNuclear Localization Signal
UseAvian, xenopus, zebrafishTagsGCamp6ExpressionMammalianMutationPromoterSP6Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Frb-NLS-mCherry
Plasmid#217613Purposepart B of SPARK-OFFDepositorInsertFrb-NLS-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_CymRV5-nls-GFP_DEx2
Plasmid#119906PurposeePiggyBac mammalian expression plasmid for CymR-GFP fusionDepositorInsertCymRV5-nls-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_CMV_MeltNLS/NES-40-mCherry
Plasmid#234066PurposeEncodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°CDepositorInsertMeltNLS/NES-40-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
mPlum-PCNA-19-SV40NLS-4
Plasmid#55994PurposeLocalization: Nucleus, Excitation: 590, Emission: 649DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTagsmPlumExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBCCP-GFP-NLS
Plasmid#173923PurposeFluorescent labeling of the nuclear envelopeDepositorInsertBiotin carboxyl carrier protein, AcGFP1
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSC-NLS-EGFP
Plasmid#221811PurposeLentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)DepositorInsertNLS-EGFP
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUGW NLS-FlpO
Plasmid#183448PurposeLentiviral FlpO vectorDepositorInsertNLS-FlpO
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-nls-sfGFP
Plasmid#132973Purposefull-length of nuclear localized sfGFP with stop codon in pmE Gateway vectorDepositorInsertnuclear localized super folder GFP
UseGateway vectorTags6x myc and SV40 NLSExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-mTagBFP2
Plasmid#216128PurposeLentiviral vector for expressing mTagBFP2 localizing to the nucleusDepositorInsertEF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-R-GECO
Plasmid#32462PurposeMammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
UseTagsNLS sequence DPKKKRKVExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
Prab-3::NLS::GFP
Plasmid#68120PurposeNuclear Localized GFP for pan-neuronal expression in C. ElegansDepositorInsertGFP
UseTagsExpressionWormMutationPromoterrab-3Available sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-nlsCRE
Plasmid#12265DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:PYL1:nls:VPR
Plasmid#135988PurposeExpresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cellsDepositorInsertPYL1 domain fused to the VPR activation domain
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19/NLS-mChe
Plasmid#183162PurposeConstruct for transient expression of cell nucleus-targeted mCherry in plants.DepositorInsertNLS-mCherry
UseTags16 aa nuclear localization signal and 16 aa nucl…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_Lifeact-GFP_NLS-mCherry
Plasmid#69058PurposeDual Fluorescent Reporter for F-actin and NucleiDepositorInsertLifeAct-GFP_IRES_NLS-mCherry
UseTagsLifeact-GFP, NLS-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAS-mCherry-NLS
Plasmid#87695PurposeGal4VP16 driven expression of mCherry-NLS in mammalian cellsDepositorInsertmCherry-NLS
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-opCas12a-6xNLS
Plasmid#209022PurposeLentiviral vector expressing human codon-optimized OpCas12a with 6xNLSDepositorInsertopCas12a-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/S542RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-UltraCas12a-6xNLS
Plasmid#209023PurposeLentiviral vector expressing human codon-optimized UltraCas12a with 6xNLSDepositorInsertUltraCas12a-6xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationM537R/F870LPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1NTD-NLS
Plasmid#174826PurposeMammalian expression of human MLH1NTD-NLS (codon optimized)DepositorInserthuman MLH1_NTD-NLS (codon optimized)
UseTagsSV40 bpNLSExpressionMammalianMutationtruncation of residues 1-335PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-NLS-mEGFP
Plasmid#105527PurposeT7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signalDepositorInsertNucleoplasmin
UseIn vitro transcriptionTagsmEGFPExpressionMutationNLS of nucleoplasmin (encoding amino acids KRPAAT…PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX
Plasmid#52421PurposeExpresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherryDepositorInsertFusion NLS-EGFP-P2A-Cherry-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-nlsABACUS2-400n
Plasmid#203726PurposeGateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acidDepositorInsertnlsABACUS2-400n
UseSynthetic BiologyTagsMycExpressionMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLew100::NLS-ISceI-HA
Plasmid#21299DepositorInsertI-SceI (SCEI )
UseTagsHA and NLSExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PCNA-19-SV40NLS-4
Plasmid#55117PurposeLocalization: Nucleus, Excitation: 587, Emission: 610DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR
Plasmid#82024PurposeLentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selectionDepositorInsertmKate2-2xNLS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-tdTomato-cNLS
Plasmid#138529PurposeInducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
UseTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-PCNA-19-SV40NLS-4
Plasmid#55320PurposeLocalization: Nucleus, Excitation: 399, Emission: 456DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTags3xSV40 NLS and mTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP
Plasmid#32627DepositorInsertDonor + Sce BFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
766 p3E-2A-nlsmCherrypA
Plasmid#195972PurposeGateway p3E 2A fluorophoreDepositorInsert2A-nlsmCherry (nuclear)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-NLS
Plasmid#199663Purposeexpresses LOV-Turbo in the mammalian nucleus, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsNLS and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
RANLS-DEVD-BNES
Plasmid#50840PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddRFP A and ddRFP B
UseTagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_CymRV5-nls-Halox2_DEx4
Plasmid#119907PurposeePiggyBac mammalian expression plasmid for CymR-Halo fusionDepositorInsertCymRV5-nls-Halox2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
GANLS-DEVD-BNES
Plasmid#50835PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddGFP A and ddRFP B
UseTagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNLS-TetR-GFP
Plasmid#103838PurposeFlourescent marker for tetO arraysDepositorInsertNLS-TetR (tetR )
UseTagsEGFPExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEX.(NLS)iGlucoSnFR.H66A.L276V
Plasmid#238944PurposeGreen glucose sensor, high sensitivity, nuclearDepositorInsertiGlucoSnFR.L276V.K312A
UseLentiviralTagsMyc and NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLEX.(NLS)iGlucoSnFR.L276V.K312A
Plasmid#238945PurposeGreen glucose sensor, moderate sensitivity, nuclearDepositorInsertiGlucoSnFR.L276V.K312A
UseLentiviralTagsMyc and NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
epB_CAG_TetRFlag-nls-GFPx2_DEx2
Plasmid#119910PurposeePiggyBac mammalian expression plasmid for TetR-GFP fusionDepositorInsertTetRFlag-nls-GFPx2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-MCP-AP_pLX304
Plasmid#120918PurposeLentiviral vector expressing NLS-MCP-AP in mammalian nucleusDepositorInsertNLS-MCP-AP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only