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Showing: 3931 - 3960 of 160192 results

  1. LPAR1-DuET

    Plasmid
    #213329
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LPAR1 (LPAR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213329-plasmid-map-sequence-id-430863

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  2. pLKO_HA.V5.FLAG_NGFR

    Plasmid
    #158250
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.V5.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158250-plasmid-map-sequence-id-327836

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  3. pRL_CT_GI_del1

    Plasmid
    #98744
    Purpose
    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    SV40-NLS, VP64-AD, and synTALE-DBD
    Expression
    Yeast
    Mutation
    mutated nucleotide 576 C to A, deleted amino acid…
    Promoter
    FBA1 and TDH3
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98744-plasmid-map-sequence-id-189858

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  6. pT26CfpSox10G4FER3(#1048)

    Plasmid
    #184073
    Purpose
    cyclofen-inducible GAL4 activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    Gal4bdFF-ERT2
    Use
    Zebrafish transgenesis (blue heart)
    Tags
    Expression
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Sox10
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    184073-plasmid-map-sequence-id-365954

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  7. pcDNA3-HCMVgH-8his (Merlin)

    Plasmid
    #136447
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Use
    Tags
    Short GGSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular domain (a.a. 1-716). Codon-optimize…
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136447-plasmid-map-sequence-id-273651

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  8. TFORF2952

    Plasmid
    #144428
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TLE1 (TLE1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144428-plasmid-map-sequence-id-391591

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  10. pLenti-CD74-ROS1 G2032R/L2086F

    Plasmid
    #183820
    Purpose
    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R/L2086F mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899
    Inserts
    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of CD74 exon 1-6 and ROS1 exon…
    Expression
    Mammalian
    Mutation
    G2032R/L2086F
    Promoter
    CMV
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183820-plasmid-map-sequence-id-360529

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  11. pLenti-CD74-ROS1 L2086F

    Plasmid
    #183816
    Purpose
    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2086F mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899
    Inserts
    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of CD74 exon 1-6 and ROS1 exon…
    Expression
    Mammalian
    Mutation
    L2086F
    Promoter
    CMV
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183816-plasmid-map-sequence-id-360533

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  12. LSB-hsa-miR-155-5p

    Plasmid
    #103265
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-155-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-155-5p target (MIR155 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103265-plasmid-map-sequence-id-195579

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  14. pAR100

    Plasmid
    #180377
    Purpose
    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).
    Depositor
    Alec Redwood
    Article
    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:
    Inserts
    NFAT-Luciferase
    hCD8A
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    hEF1-HTLV and minimal promoter and an NFAT respon…
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    180377-plasmid-map-sequence-id-370772

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  16. qTAG-C-miRFP670nano3-Blast-H3C2

    Plasmid
    #207784
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207784-plasmid-map-sequence-id-416184

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  17. HSC1-GiP

    Plasmid
    #58254
    Purpose
    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Available since
    Oct. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58254-plasmid-map-sequence-id-321491

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  18. TFORF1511

    Plasmid
    #143512
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT5A (STAT5A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143512-plasmid-map-sequence-id-400413

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  21. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

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  25. pcDNA3-Flag-Hsp40-S171A

    Plasmid
    #84999
    Purpose
    Hsp40/DnaJB1 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp40 (DNAJB1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Serine 171 mutated to Alanine
    Promoter
    CMV
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. pLenti- V6.3 Ultra-Chili

    Plasmid
    #106173
    Purpose
    lentiviral CMV driven tdTomato-P2A-T2A, Blasticidin selectable
    Depositor
    Ewa Snaar-Jagalska
    Insert
    dTomato
    Use
    Lentiviral
    Tags
    T2A, P2A
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106173-plasmid-map-sequence-id-207442

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  29. pJT9R

    Plasmid
    #98305
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    TRPL3AcNES1>TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98305-plasmid-map-sequence-id-190176

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Showing: 3931 - 3960 of 160192 results