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165,995 results

Showing: 5321 - 5360 of 165995 results

  1. AAV.CamKII.GCaMP6s.WPRE.SV40

    Plasmid
    #107790
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from the CamKII promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CamKII
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107790-plasmid-map-sequence-id-207334

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  4. Platinum Gate TALEN Kit

    Plasmid Kit
    #1000000043
    Purpose
    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.
    Depositor
    Takashi Yamamoto
    Available Since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

    Plasmid
    #188072
    Purpose
    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.
    Depositor
    Kristen Verhey
    Article
    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e
    Insert
    rat KIF5C (Kif5c Rat)
    Use
    Tags
    Flag and Halo
    Expression
    Mammalian
    Mutation
    aa1-560, deletion of aa2-6
    Promoter
    CMV
    Available Since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    188072-plasmid-map-sequence-id-368400

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  7. pFastBac HT JS-Munc18c

    Plasmid
    #135555
    Purpose
    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.
    Depositor
    Jingshi Shen
    Article
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18c
    Use
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135555-plasmid-map-sequence-id-272498

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  9. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    92308-plasmid-map-sequence-id-216421

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  11. pYPQAT09

    Plasmid
    #223381
    Purpose
    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-SpRY-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223381-plasmid-map-sequence-id-453508

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  16. pcDNA3-BG4-NLS-mCer

    Plasmid
    #211448
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Use
    Tags
    NLS-mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211448-plasmid-map-sequence-id-419419

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  18. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV Retrograde)

    Viral Prep
    #20297-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only

  23. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid
    #200904
    Purpose
    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Insert
    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200904-plasmid-map-sequence-id-393155

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  26. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid
    #216154
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216154-plasmid-map-sequence-id-437765

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  27. pLV-TetO-hNGN2-eGFP-Puro

    Plasmid
    #79823
    Purpose
    Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.
    Depositor
    Kristen Brennand
    Article
    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15
    Insert
    hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TetO
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79823-plasmid-map-sequence-id-240610

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  30. pBHSTC104

    Plasmid
    #124356
    Purpose
    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    gp41-1 N-intein and C-intein (bicistronic)
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Promoter
    STC
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124356-plasmid-map-sequence-id-242355

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  32. pRRLsinPGK_IRFP720_WPRE

    Plasmid
    #91785
    Purpose
    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    iRFP720
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91785-plasmid-map-sequence-id-180617

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  33. pAAV_BiPVe4_dTomato_nlsdTomato (AAV PHP.eB)

    Viral Prep
    #213936-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiPVe4_dTomato_nlsdTomato (#213936). In addition to the viral particles, you will also receive purified pAAV_BiPVe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the chandelier cell-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Promoter
    Tags
    dTomato
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 5321 - 5360 of 165995 results