167,069 results

1. peGFP C3-SPG20 8xV

   Plasmid

   #218579

   Purpose

   Expresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Tryptophan 419, Leucine 438, Isoleucine 4…

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BL21ΔF

   Bacterial Strain

   #102259

   Purpose

   ompF deletion strain for overproduction of outer membrane proteins

   Depositor

   Jack Leo

   Article

   Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

   Bacterial Resistance

   None

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pLenti puro HA-Ubiquitin

   Plasmid

   #74218

   Purpose

   Lentiviral expression of HA tagged Ubiquitin

   Depositor

   Melina Fan

   Insert

   ubiquitin (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Single ubiquitin coding sequence (full length UBB…

   Promoter

   CMV

   Available Since

   March 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pcw57.1-RquA-FLAG

   Plasmid

   #229685

   Purpose

   Doxycycline-inducible expression of RquA-FLAG

   Depositor

   Jessica Spinelli

   Article

   Valeros et al Cell. 2025 Jan 10:S0092-8674(24)01420-X.

   Insert

   RquA-FLAG
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiGuide-Hygro-mTagBFP2

   Plasmid

   #99374

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TLR4

   Plasmid

   #20863

   Depositor

   Jie-Oh Lee

   Article

   Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

   Insert

   Toll-Like Receptor 4 (TLR4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Contains ectodomain (amino acids 27–631)

   Promoter

   Available Since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer20 ALPK1 Y254C

   Plasmid

   #231587

   Purpose

   ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

   Depositor

   Thomas Henry

   Article

   Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   Lentiviral

   Tags

   3X flag

   Expression

   Mammalian

   Mutation

   changed tyrosine 254 to cysteine

   Promoter

   doxycycline

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Rosa26-LSL-PE2 Targeting Vector

   Plasmid

   #196038

   Purpose

   The targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA.

   Depositor

   Tyler Jacks

   Article

   Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

   Insert

   Cas9-MMLV-RT
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E ubb polyA

   Plasmid

   #195977

   Purpose

   Gateway p3E vector

   Depositor

   Christian Mosimann, Alexa Burger

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   ubb:polyA (ubb Zebrafish)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. opto-Rac1

    Plasmid

    #135396

    Purpose

    Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3

    Insert

    opto-Rac1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCherry-TOMM20-N-10

    Plasmid

    #55146

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET MBP mCherry LIC cloning vector (MBP-mCherry)

    Plasmid

    #29747

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CAG-GFP (AAV Retrograde trial size)

    Viral Prep

    #37825-AAVrg.T

    Purpose

    Ready-to-use AAV Retrograde trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

    Plasmid

    #40810

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(31-51), S218E, S222D

    Promoter

    CMV

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

15. Human Brunello kinome pooled library, guides 1-4 in lentiGuide-Puro backbone

    Pooled Library

    #75312

    Purpose

    Human sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

16. 3XFLAG-VP64-SadCas9-NLS-VP64

    Plasmid

    #135338

    Purpose

    3xFLAG 2xVP64 SadCas9 for single guide cloning

    Depositor

    Ronald Cohn

    Article

    Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

    Insert

    SadCas9
    

    Use

    Tags

    3xFLAG-VP64-SV40 NLS and NLS-VP64

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His-GFP-Syntaxin1A

    Plasmid

    #170871

    Purpose

    Mammalian expression of His-GFP-Syntaxin1A.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Syntaxin 1A (Stx1a Mouse)
    

    Use

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit HC

    Plasmid

    #221355

    Purpose

    Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit HC; to be used with Anti-Strep [IPI-C23.21] light chain (Plasmid 221356) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-Strep [IPI-C23.21] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa

    Plasmid

    #221356

    Purpose

    Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa; to be used with Anti-Strep [IPI-C23.21] heavy chain (Plasmid 221355) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCAD29_hIRG1_4-461_pvp008

    Plasmid

    #124843

    Purpose

    Expresses human CAD (Irg1) in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 462-end.

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mCherry-LaminB1-10

    Plasmid

    #55069

    Purpose

    Localization: Nuclear Envelope, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TUBB5-Halo

    Plasmid

    #64691

    Purpose

    Expresses beta-tubulin labeled with Halo-tag

    Depositor

    Yasushi Okada

    Article

    Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

    Insert

    TUBB
    

    Use

    Tags

    Halo-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. plenti-CAG-gate-FLAG-IRES-GFP

    Plasmid

    #107398

    Purpose

    Lentiviral gateway destination vector

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFRT_TO_eGFP_TIA1

    Plasmid

    #106094

    Purpose

    gene expression of EGFP-TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIA1 (TIA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE-NRF2-E79Q

    Plasmid

    #207828

    Purpose

    FLAG-tagged NRF2-E79Q driven by CMV promoter

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    NRF2-E79Q (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    E79Q

    Promoter

    CMV

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid

    #58475

    Purpose

    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHCMV_FLUAV_HA

    Plasmid

    #207273

    Purpose

    Mammalian expression of Influenza A virus HA protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    FLUAV_HA (HA Influenza A virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKL003

    Plasmid

    #98919

    Purpose

    Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    PROPS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. siRNA-resistant pLV-SigmaR1-mNeonGreen

    Plasmid

    #226569

    Purpose

    Encodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector)

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOPINF

    Plasmid

    #26042

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    His3C-lacZ
    

    Use

    Tags

    MAHHHHHHSSGLEVL

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. gag-PE

    Plasmid

    #211371

    Purpose

    Packaging plasmid for v3 PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-PE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFP hsp70

    Plasmid

    #15215

    Depositor

    Lois Greene

    Article

    Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

    Insert

    hsp70 (HSPA1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. hE-cadherin/Δβ-catenin-pcDNA3

    Plasmid

    #45772

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin/Δβ-catenin (CDH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ends at amino acid 844. Last 35 amino acids dele…

    Promoter

    CMV

    Available Since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LentiCRISPR v2 hBAK

    Plasmid

    #129579

    Purpose

    CRISPR deletion of human BAK

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs human BAK (BAK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCEFLmGFP-Gli2

    Plasmid

    #37672

    Depositor

    Philip Beachy

    Article

    Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP_TRPM8

    Plasmid

    #64879

    Purpose

    Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

    Depositor

    Sebastián Brauchi

    Article

    Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

    Insert

    Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

    Plasmid

    #192204

    Purpose

    Lenti-GL(GFP-Luciferase)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-GL(GFP-Luciferase)
    

    Use

    Lentiviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCS2-Tol1.zf1

    Plasmid

    #61388

    Purpose

    mRNA synthesis of tol1 transposase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    tol1.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGP-AAV-syn-FLEX-Volton2-ST-WPRE (AAV1)

    Viral Prep

    #172907-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-Volton2-ST-WPRE (#172907). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-Volton2-ST-WPRE plasmid DNA. Cre-dependent, Syn-driven expression of the voltage sensor Voltron2-ST containing a soma localization targeting sequence. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Promoter

    Syn

    Tags

    None

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

40. nAChR beta2 WT

    Plasmid

    #24272

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    nAChR beta2 WT (Chrnb2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. lenti U6-sgRNA/EF1a-mCherry

    Plasmid

    #114199

    Purpose

    Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLV-Ftractin-mCherry

    Plasmid

    #85131

    Purpose

    lentiviral expression of an F-actin live-cell marker (mCherry)

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. MSCV-opEBNA2-IRES-GFP

    Plasmid

    #220354

    Purpose

    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    3 x HA tag

    Expression

    Mammalian

    Mutation

    codon-optimised for expression in mammalian cells

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLenti_Ef1a:PE2-P2A-hMLH1dn

    Plasmid

    #184444

    Purpose

    Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Ef1A

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. nAChR alpha4 WT

    Plasmid

    #24271

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    nAChR alpha4 WT (Chrna4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

46. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

    Plasmid

    #126217

    Purpose

    Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    Gephyrin.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pET28b-ULP1-405-621

    Plasmid

    #229224

    Purpose

    For expression of ULP1 protein

    Depositor

    Andy LiWang

    Insert

    ULP1 (ULP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    405-621 (truncated)

    Promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

    Plasmid

    #224006

    Purpose

    AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAct-dCBEevoCDA1

    Plasmid

    #195515

    Purpose

    Ubiquitous expression of dCBEevoCDA1 in Drosophila

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    dCBE-evoCDA1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. M31: pHR-SFFVp- TDP-43C::mCherry::SspB

    Plasmid

    #122669

    Purpose

    C terminal domain of TDP43 (218-414) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    TDP43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry-SspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 218-414

    Promoter

    SFFV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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